##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062311_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1059031
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.249609312664123	32.0	32.0	32.0	32.0	32.0
2	31.447017131698694	32.0	32.0	32.0	32.0	32.0
3	31.565797412918034	32.0	32.0	32.0	32.0	32.0
4	31.64556656037453	32.0	32.0	32.0	32.0	32.0
5	31.628170469041983	32.0	32.0	32.0	32.0	32.0
6	35.26306878646612	36.0	36.0	36.0	36.0	36.0
7	35.26515937682655	36.0	36.0	36.0	36.0	36.0
8	35.216109821147825	36.0	36.0	36.0	36.0	36.0
9	35.2899745144382	36.0	36.0	36.0	36.0	36.0
10	35.17632439465889	36.0	36.0	36.0	36.0	36.0
11	35.28845614528753	36.0	36.0	36.0	36.0	36.0
12	35.21544789529296	36.0	36.0	36.0	36.0	36.0
13	35.25467998576057	36.0	36.0	36.0	36.0	36.0
14	35.21317695138291	36.0	36.0	36.0	36.0	36.0
15	35.199475747168876	36.0	36.0	36.0	36.0	36.0
16	35.20541986023072	36.0	36.0	36.0	36.0	36.0
17	35.1910860022039	36.0	36.0	36.0	36.0	36.0
18	35.18366129036827	36.0	36.0	36.0	36.0	36.0
19	35.17061256941487	36.0	36.0	36.0	36.0	36.0
20	35.17275698256236	36.0	36.0	36.0	36.0	36.0
21	35.16266001656231	36.0	36.0	36.0	36.0	36.0
22	35.14935728982438	36.0	36.0	36.0	36.0	36.0
23	35.12056965282414	36.0	36.0	36.0	36.0	36.0
24	35.104471918196914	36.0	36.0	36.0	36.0	36.0
25	35.097313487518306	36.0	36.0	36.0	36.0	36.0
26	35.045657775834705	36.0	36.0	36.0	36.0	36.0
27	35.04526024261801	36.0	36.0	36.0	36.0	36.0
28	35.00938499439582	36.0	36.0	36.0	36.0	36.0
29	35.005163210519804	36.0	36.0	36.0	36.0	36.0
30	34.975040390696776	36.0	36.0	36.0	36.0	36.0
31	34.98487013128039	36.0	36.0	36.0	36.0	36.0
32	34.95635821803139	36.0	36.0	36.0	36.0	36.0
33	34.93112005219866	36.0	36.0	36.0	36.0	36.0
34	34.91864921801156	36.0	36.0	36.0	36.0	36.0
35	34.900414624312226	36.0	36.0	36.0	36.0	36.0
36	34.885231877064975	36.0	36.0	36.0	32.0	36.0
37	34.87544557241478	36.0	36.0	36.0	32.0	36.0
38	34.85410719799515	36.0	36.0	36.0	32.0	36.0
39	34.844133929979385	36.0	36.0	36.0	32.0	36.0
40	34.83089352436331	36.0	36.0	36.0	32.0	36.0
41	34.799204178159094	36.0	36.0	36.0	32.0	36.0
42	34.76927776429585	36.0	36.0	36.0	32.0	36.0
43	34.75028965157772	36.0	36.0	36.0	32.0	36.0
44	34.709503310101404	36.0	36.0	36.0	32.0	36.0
45	34.69130082122242	36.0	36.0	36.0	32.0	36.0
46	34.66629494320752	36.0	36.0	36.0	32.0	36.0
47	34.6323582595788	36.0	36.0	36.0	32.0	36.0
48	34.60981973143374	36.0	36.0	36.0	32.0	36.0
49	34.58630578330568	36.0	36.0	36.0	32.0	36.0
50	34.19286970825217	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	10.0
21	53.0
22	113.0
23	262.0
24	637.0
25	1438.0
26	2900.0
27	5425.0
28	9102.0
29	14064.0
30	20409.0
31	29427.0
32	42897.0
33	71379.0
34	162983.0
35	697932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.313777719843614	17.748351001678518	11.806384029818462	26.131487248659408
2	16.37752105323792	19.749816333044375	37.269283141734235	26.60337947198347
3	19.068069086057296	23.2571029822658	28.158111171858696	29.516716759818213
4	12.661952294125477	15.750247159903724	35.38763265664556	36.20016788932524
5	14.412986966387198	36.74594983527395	33.22745037680672	15.613612821532136
6	34.82712026371277	34.98518929096504	16.37355280440327	13.814137640918917
7	30.77435882424594	30.060687553055576	19.933977381209804	19.23097624148868
8	28.067639190920758	33.13831228736458	18.89736938767609	19.89667913403857
9	27.1805074638986	14.333858026818854	18.45573925598023	40.029895253302314
10	16.222124228892692	26.66924135221982	31.112368027693293	25.9962663911942
11	37.339511307978704	21.390497539732074	21.817680502270473	19.452310650018745
12	24.705131388977282	23.62149927622515	28.423436141151676	23.249933193645887
13	29.573827395043207	19.46845748613591	25.033167112199738	25.924548006621144
14	23.618949775785598	19.285271158256933	25.168007357669413	31.92777170828805
15	25.279618821356504	26.932450513724337	22.159219135228337	25.628711529690822
16	25.67505807255765	26.044172914581402	23.517875016524712	24.762893996336235
17	24.047926831225904	25.989796332685255	25.053090986005135	24.90918585008371
18	24.763604430469393	25.13450988168418	25.934392793405287	24.16749289444114
19	25.416725289439118	24.950166709000964	25.54221736663044	24.09089063492948
20	25.804700159013738	24.228160161959835	25.137484561314714	24.829655117711713
21	26.54957838776994	24.19572627782027	24.858785870088663	24.395909464321125
22	25.740511845262322	24.37936188836776	25.25544578015186	24.62468048621806
23	24.41026222108911	24.439723142876026	25.719290293948237	25.430724342086624
24	24.693157975248674	24.69032515150886	25.572277609172307	25.04423926407016
25	24.98784265994102	24.32110108202687	25.547033089682923	25.14402316834918
26	24.549350063171254	25.21788889035774	25.625716460219106	24.607044586251895
27	25.103325977524655	24.823446427711474	25.250725430040973	24.822502164722902
28	24.73135438935767	24.66506643903397	25.697439887593767	24.906139284014593
29	24.470904841717616	24.98297962748755	25.671631925591935	24.874483605202897
30	24.734659344145886	24.963362215490605	25.759856319451263	24.542122120912243
31	25.04105191095583	24.838223837964165	25.062770000708202	25.057954250371807
32	24.92691383764674	24.827293810149722	25.02861124960341	25.21718110260013
33	24.57354803315112	24.58478474149433	25.532067582662986	25.30959964269156
34	25.10948781045153	24.72299914449369	25.640827102741966	24.526685942312813
35	25.570380442480385	24.47343323607452	25.640916687912522	24.315269633532573
36	24.621564430125275	24.974811879916643	25.321543939695818	25.08207975026227
37	25.362978463026053	24.859775489699874	25.242394826895907	24.53485122037816
38	24.67189345732089	24.715234964793286	25.48754474609336	25.12532683179246
39	25.230778528154225	24.765161129492817	25.080451434528396	24.923608907824562
40	25.500221901173713	24.79013814528389	25.193526151289387	24.516113802253006
41	24.520099223716997	25.30790229844356	25.54935171257227	24.622646765267167
42	25.52911814115294	25.06773033270726	25.10597471715681	24.297176808982993
43	25.12582791462777	24.43875556428508	25.414972435896228	25.02044408519093
44	24.677849775916023	25.074930735104978	25.23140235207908	25.015817136899916
45	24.911332114582706	25.22351105940654	25.085079621683686	24.78007720432707
46	24.70517247728773	24.924811781580704	25.15531029481336	25.21470544631821
47	24.717450239231287	24.98940054786656	25.416966866443126	24.87618234645903
48	25.282296216975737	25.395800165062315	24.706655561787887	24.615248056174067
49	24.890580219925496	25.464048461040896	24.74006015136993	24.905311167663683
50	25.048866209511868	25.38804963862612	24.859067018944035	24.70401713291798
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	187.0
1	116.5
2	46.0
3	112.0
4	178.0
5	208.0
6	238.0
7	194.0
8	150.0
9	220.0
10	290.0
11	503.0
12	716.0
13	1175.0
14	1634.0
15	2216.0
16	2798.0
17	2757.5
18	2717.0
19	2624.0
20	2531.0
21	2830.5
22	3130.0
23	3704.5
24	4279.0
25	5128.5
26	5978.0
27	7553.0
28	9128.0
29	10896.0
30	12664.0
31	14714.5
32	16765.0
33	19187.5
34	21610.0
35	24554.5
36	27499.0
37	32230.0
38	36961.0
39	41199.5
40	45438.0
41	49925.5
42	54413.0
43	57443.0
44	60473.0
45	65338.0
46	70203.0
47	74458.5
48	78714.0
49	81853.5
50	84993.0
51	82569.0
52	80145.0
53	79198.0
54	78251.0
55	78303.0
56	78355.0
57	74855.0
58	71355.0
59	65031.0
60	58707.0
61	51608.5
62	44510.0
63	38409.0
64	32308.0
65	27210.5
66	22113.0
67	19255.0
68	16397.0
69	14510.0
70	12623.0
71	10003.0
72	7383.0
73	6414.5
74	5446.0
75	4050.0
76	2654.0
77	2286.5
78	1919.0
79	1655.0
80	1391.0
81	1111.0
82	831.0
83	658.5
84	486.0
85	368.5
86	251.0
87	172.5
88	94.0
89	65.0
90	36.0
91	26.0
92	16.0
93	15.5
94	15.0
95	9.0
96	3.0
97	4.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0338044873096255
2	0.004626871168077233
3	3.777037688226313E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.8885188441131564E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.001038685364262236
17	0.0
18	9.442594220565782E-5
19	0.0
20	2.8327782661697346E-4
21	9.442594220565782E-5
22	0.0
23	9.442594220565782E-5
24	0.0016052410174961828
25	0.0
26	4.7212971102828904E-4
27	3.777037688226313E-4
28	0.0014163891330848672
29	5.665556532339469E-4
30	0.0014163891330848672
31	5.665556532339469E-4
32	6.609815954396047E-4
33	1.8885188441131564E-4
34	8.498334798509204E-4
35	9.442594220565782E-5
36	0.0
37	0.0014163891330848672
38	0.0
39	6.609815954396047E-4
40	9.442594220565782E-5
41	9.442594220565781E-4
42	0.004910148994694206
43	0.0042491673992546015
44	0.0050045749368998645
45	0.00217179667073013
46	0.002077370728524472
47	0.0013219631908792094
48	0.003493759861609339
49	0.0033993339194036817
50	0.0016052410174961828
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1059031.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.98090002297885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.54700290573945	43.223023224903926
2	15.452821969356773	17.919370513563326
3	4.939254958859137	8.591473438728425
4	1.9839589167385652	4.601268944044647
5	0.9671253586032482	2.8037399363431255
6	0.5287149639173522	1.8393221678126923
7	0.3271463314925653	1.327776711740831
8	0.20541556239333159	0.9528143349033389
9	0.16502566552384984	0.8611502952567497
>10	0.7674627409593013	7.996611533087031
>50	0.062966565125221	2.5769516906071983
>100	0.04900652698065397	5.596120436115142
>500	0.003933632938197397	1.6078377942319932
>1k	1.6390137242489156E-4	0.10253897866164198
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1079	0.10188559163990477	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.442594220565782E-5	0.0
2	0.0	0.0	0.0	9.442594220565782E-5	0.0
3	0.0	0.0	0.0	9.442594220565782E-5	0.0
4	0.0	0.0	0.0	9.442594220565782E-5	0.0
5	0.0	0.0	0.0	9.442594220565782E-5	0.0
6	0.0	0.0	0.0	9.442594220565782E-5	1.8885188441131564E-4
7	0.0	0.0	0.0	9.442594220565782E-5	1.8885188441131564E-4
8	0.0	0.0	0.0	9.442594220565782E-5	1.8885188441131564E-4
9	0.0	0.0	0.0	9.442594220565782E-5	1.8885188441131564E-4
10	0.0	0.0	0.0	9.442594220565782E-5	1.8885188441131564E-4
11	0.0	0.0	0.0	1.8885188441131564E-4	1.8885188441131564E-4
12	0.0	0.0	0.0	1.8885188441131564E-4	3.777037688226313E-4
13	0.0	0.0	0.0	1.8885188441131564E-4	3.777037688226313E-4
14	0.0	0.0	0.0	1.8885188441131564E-4	3.777037688226313E-4
15	0.0	0.0	0.0	1.8885188441131564E-4	4.721297110282891E-4
16	0.0	0.0	0.0	1.8885188441131564E-4	6.609815954396047E-4
17	0.0	0.0	0.0	1.8885188441131564E-4	6.609815954396047E-4
18	0.0	0.0	0.0	1.8885188441131564E-4	7.554075376452626E-4
19	0.0	0.0	0.0	1.8885188441131564E-4	7.554075376452626E-4
20	0.0	0.0	0.0	2.8327782661697346E-4	7.554075376452626E-4
21	0.0	0.0	0.0	5.665556532339469E-4	7.554075376452626E-4
22	0.0	0.0	0.0	7.554075376452626E-4	7.554075376452626E-4
23	0.0	0.0	0.0	0.001038685364262236	7.554075376452626E-4
24	0.0	0.0	0.0	0.0016996669597018406	7.554075376452626E-4
25	0.0	0.0	0.0	0.0021717966707301296	7.554075376452626E-4
26	0.0	0.0	0.0	0.0032104820349923655	7.554075376452626E-4
27	0.0	0.0	0.0	0.003493759861609339	7.554075376452626E-4
28	0.0	0.0	0.0	0.0042491673992546015	7.554075376452626E-4
29	0.0	0.0	0.0	0.00651539001219039	7.554075376452626E-4
30	0.0	0.0	0.0	0.010858983353650648	7.554075376452626E-4
31	0.0	0.0	0.0	0.02067928134303906	7.554075376452626E-4
32	0.0	0.0	0.0	0.032954653829774576	7.554075376452626E-4
33	0.0	0.0	0.0	0.042586099934751674	7.554075376452626E-4
34	0.0	0.0	0.0	0.055050324305898506	7.554075376452626E-4
35	0.0	0.0	0.0	0.06836438215689626	7.554075376452626E-4
36	0.0	0.0	0.0	0.08356695885200717	7.554075376452626E-4
37	0.0	0.0	0.0	0.11019507455400267	8.498334798509203E-4
38	0.0	0.0	0.0	0.1435274321525999	8.498334798509203E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	870	0.0	24.287956	1
GTATCAA	1745	0.0	24.092224	1
CGCAATA	185	0.0	20.217691	36
TACGAAT	210	0.0	19.906214	41
GTAGGGG	100	1.1239063E-6	19.798641	3
TAGACCG	60	0.007414448	18.332075	5
TTAACGG	60	0.007414448	18.332075	3
TATTAGG	145	6.2991603E-9	18.208225	2
ATACGAA	230	0.0	18.175238	40
CGACGGT	235	0.0	17.786013	7
TACGACG	260	0.0	17.76801	5
TAGGACG	100	2.4840076E-5	17.598791	4
TAACGCC	240	0.0	17.41547	4
TTAGGAC	165	1.6607373E-9	17.332144	3
CAATACG	220	1.8189894E-12	17.00124	38
GGCGTTA	285	0.0	16.983696	42
CTAGCGG	235	0.0	16.850702	29
ATAACGC	250	0.0	16.718851	3
TATCAAC	2575	0.0	16.575966	2
ATCGTTT	240	0.0	16.499645	29
>>END_MODULE