FastQCFastQC Report
Thu 2 Feb 2017
SRR4062310_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062310_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295149
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24220.18700551056287731No Hit
TATCAACGCAGAGTACTTTTTTTTT15600.12044946179937599No Hit
GCGCAAGACGGACCAGAGCGAAAGC13940.10763240368482699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAC653.3616052E-614.61934311
CGCGTAA653.3629549E-614.61877710
GGTATCA6400.013.9482711
CAAGACG2250.013.0873714
AAGACGG2400.013.0619945
ACGGTAT1102.739398E-912.9560499
AGTGACG1102.746674E-912.95354419
CGGTCCA2300.012.80732110
TCGCGTA751.476366E-512.6681379
ATTATAC751.4804813E-512.6647073
TACACTA701.091021E-412.2138145
ATCGCCA1950.012.17854516
CGCATCG1800.012.14076713
GTATCAA15000.012.0924591
GTATTAG1353.7289283E-1011.95881
TATTAAG658.0348435E-411.6900472
GTTTAGA907.4866875E-611.6070711
GTATTAA1406.8394E-1011.53171
CATCGCC1900.011.50133515
GGTCCAA2900.011.46862211
Produced by FastQC (version 0.11.5)