FastQCFastQC Report
Thu 2 Feb 2017
SRR4062310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295149
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14040.10840451561943838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8200.027.911281
GTATCAA17500.025.1508241
AATTCCG2950.019.3922342
ATTCCGA3150.018.86166243
TGTACGC600.007412044318.3334772
CGCAATA3250.018.27636136
CATATAG2300.018.1740572
TCTAGCG3300.018.00014328
GTCCTAA2003.6379788E-1217.6055771
ATACGAA3400.017.47207540
TAGCGGC3400.017.4700530
GCATATA2650.017.4394861
TCAACGC25850.017.1051984
ATCAACG25950.017.0392823
GTCCTAC3400.016.828861
CAACGCA26050.016.720535
CGTCGTA2900.016.68849810
AACGCAG26150.016.5724646
CTAGCGG3600.016.50013229
TATCAAC27150.016.4494862
Produced by FastQC (version 0.11.5)