##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062310_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1295149
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25824827876947	32.0	32.0	32.0	32.0	32.0
2	31.440401837935248	32.0	32.0	32.0	32.0	32.0
3	31.559791190048404	32.0	32.0	32.0	32.0	32.0
4	31.638312657462578	32.0	32.0	32.0	32.0	32.0
5	31.61612061623798	32.0	32.0	32.0	32.0	32.0
6	35.2526473787958	36.0	36.0	36.0	36.0	36.0
7	35.25045844146118	36.0	36.0	36.0	36.0	36.0
8	35.20372096183528	36.0	36.0	36.0	36.0	36.0
9	35.28585050831989	36.0	36.0	36.0	36.0	36.0
10	35.16873888641384	36.0	36.0	36.0	36.0	36.0
11	35.29097424311797	36.0	36.0	36.0	36.0	36.0
12	35.20948941009876	36.0	36.0	36.0	36.0	36.0
13	35.248338994200665	36.0	36.0	36.0	36.0	36.0
14	35.209874693954134	36.0	36.0	36.0	36.0	36.0
15	35.18482043378793	36.0	36.0	36.0	36.0	36.0
16	35.202484810627965	36.0	36.0	36.0	36.0	36.0
17	35.18332099241091	36.0	36.0	36.0	36.0	36.0
18	35.175356657805395	36.0	36.0	36.0	36.0	36.0
19	35.17036263781233	36.0	36.0	36.0	36.0	36.0
20	35.1674641296098	36.0	36.0	36.0	36.0	36.0
21	35.15957700619774	36.0	36.0	36.0	36.0	36.0
22	35.13968817487409	36.0	36.0	36.0	36.0	36.0
23	35.11005065826403	36.0	36.0	36.0	36.0	36.0
24	35.09480762445093	36.0	36.0	36.0	36.0	36.0
25	35.080151395708135	36.0	36.0	36.0	36.0	36.0
26	35.04271168799883	36.0	36.0	36.0	36.0	36.0
27	35.025287437970455	36.0	36.0	36.0	36.0	36.0
28	35.004633443719605	36.0	36.0	36.0	36.0	36.0
29	34.98388139125305	36.0	36.0	36.0	36.0	36.0
30	34.96376478690869	36.0	36.0	36.0	36.0	36.0
31	34.96672043139438	36.0	36.0	36.0	36.0	36.0
32	34.9364243033041	36.0	36.0	36.0	36.0	36.0
33	34.92497465542575	36.0	36.0	36.0	32.0	36.0
34	34.90202053972168	36.0	36.0	36.0	32.0	36.0
35	34.891943706863074	36.0	36.0	36.0	32.0	36.0
36	34.87251891481212	36.0	36.0	36.0	32.0	36.0
37	34.860091773224546	36.0	36.0	36.0	32.0	36.0
38	34.84223591262472	36.0	36.0	36.0	32.0	36.0
39	34.82888300882755	36.0	36.0	36.0	32.0	36.0
40	34.81796457396022	36.0	36.0	36.0	32.0	36.0
41	34.785993735083764	36.0	36.0	36.0	32.0	36.0
42	34.757725945045706	36.0	36.0	36.0	32.0	36.0
43	34.75359900675521	36.0	36.0	36.0	32.0	36.0
44	34.704382275707275	36.0	36.0	36.0	32.0	36.0
45	34.69258981012995	36.0	36.0	36.0	32.0	36.0
46	34.66822118536169	36.0	36.0	36.0	32.0	36.0
47	34.65036223631412	36.0	36.0	36.0	32.0	36.0
48	34.62921177408931	36.0	36.0	36.0	32.0	36.0
49	34.60990511516435	36.0	36.0	36.0	32.0	36.0
50	34.187344467702175	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	8.0
21	25.0
22	97.0
23	241.0
24	639.0
25	1469.0
26	3035.0
27	6030.0
28	10098.0
29	16400.0
30	24616.0
31	36506.0
32	55441.0
33	93524.0
34	210829.0
35	836187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.796007738915854	17.826615846241182	11.982266915362366	26.395109499480597
2	16.23993989611541	19.636689622582505	37.024116452048546	27.099254029253544
3	18.75138015754257	22.832168210126767	28.445606736558616	29.970844895772046
4	12.71730125259719	15.583071909100807	35.08221833935709	36.61740849894491
5	14.68865744404698	36.44623128304156	33.17162735716122	15.693483915750233
6	35.45510348262164	34.673646580112646	16.12630246034228	13.744947476923434
7	31.188149008338033	29.968598207619358	19.818723559991938	19.024529224050667
8	28.32708823463555	33.34550696483571	18.71885010913802	19.60855469139072
9	27.097886034734227	14.239828776457381	18.62920791352964	40.03307727527875
10	16.541597208656626	26.611264976099235	30.782451721696457	26.064686093547685
11	37.35809547781761	21.17725450894067	21.786605247735974	19.678044765505746
12	24.312878286590962	23.57188246294442	28.47610583801555	23.63913341244907
13	29.63311557203071	19.207519752553566	25.084372531654658	26.074992143761065
14	23.474133092022615	19.459614299204183	24.93921548794772	32.12703712082548
15	25.263965767645267	27.11355990700684	21.841270772706462	25.781203552641436
16	25.921173122329442	25.782037799716168	23.46607553457459	24.830713543379797
17	24.06294565335726	26.14324683878071	24.893815306192568	24.89999220166946
18	24.924255760731594	24.99922788746923	25.914258447683196	24.16225790411598
19	25.688472909294607	24.95620195050917	25.247596994631504	24.107728145564717
20	25.793731681630206	24.39342839582812	24.927266999601592	24.88557292294008
21	26.600337104070654	24.09908049189707	24.51887775074528	24.781704653286994
22	25.915551029263817	24.30237756428025	25.05341084307674	24.728660563379194
23	24.584661687574172	24.290178195713388	25.521310675451243	25.6038494412612
24	24.81287650544268	24.721302969422467	25.44794592683074	25.01787459830411
25	25.041887169477416	24.383660220546563	25.413428293026424	25.161024316949593
26	24.55533902021706	25.192024979461742	25.727872730754264	24.524763269566936
27	25.266495051523147	24.753579905277086	25.129136020186145	24.850789023013622
28	24.721147684016277	24.618146440897824	25.53952112915306	25.12118474593284
29	24.613093215889506	24.81268492152224	25.529670831969263	25.04455103061899
30	24.68553471259753	24.89933481837801	25.60304524238786	24.812085226636604
31	25.232155472939322	24.58991078219041	24.96052565542854	25.217408089441722
32	25.135255543604906	24.651833275295896	24.845016874610565	25.367894306488637
33	24.706249334051915	24.520401560905103	25.271127733861665	25.502221371181317
34	25.262250045941048	24.52688439378661	25.47720783422307	24.73365772604927
35	25.607537683010257	24.513066480535787	25.48222362575339	24.397172210700568
36	24.591937267207353	24.94409888012453	25.21804525211096	25.24591860055716
37	25.574769443638	24.710839391936553	25.04053653384067	24.673854630584778
38	24.847334167728963	24.612534928413645	25.405571096452995	25.1345598074044
39	25.423622810851043	24.486888655880687	24.86545835270064	25.22403018056763
40	25.63620093132142	24.618402979116688	25.202274024069816	24.543122065492078
41	24.64606033652064	25.02308637857337	25.559477923553974	24.771375361352014
42	25.639091300080846	24.981680682521016	25.14159346017739	24.237634557220744
43	25.165277578307055	24.340240940039873	25.255772940033694	25.23870854161937
44	24.711989572941537	24.92564234818839	25.20994647484503	25.152421604025044
45	24.875049898966655	25.112402972086688	25.047775606373783	24.964771522572864
46	24.804399307241916	24.767028050834174	25.0914785041923	25.337094137731608
47	25.02312502026817	24.790176151656894	25.38826098303342	24.79843784504152
48	25.58769819974596	25.180701029654195	24.58159440041078	24.65000637018906
49	25.00291483058078	25.207068792318125	24.712358341164126	25.077658035936967
50	25.07057217510547	25.41131069670334	24.724892057001316	24.793225071189877
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	276.0
1	166.5
2	57.0
3	168.5
4	280.0
5	283.5
6	287.0
7	235.0
8	183.0
9	244.0
10	305.0
11	490.5
12	676.0
13	1281.0
14	1886.0
15	2333.5
16	2781.0
17	2791.5
18	2802.0
19	2669.0
20	2536.0
21	3024.5
22	3513.0
23	4291.5
24	5070.0
25	6384.0
26	7698.0
27	9759.0
28	11820.0
29	13635.5
30	15451.0
31	17531.0
32	19611.0
33	22885.0
34	26159.0
35	30099.0
36	34039.0
37	40028.0
38	46017.0
39	50937.5
40	55858.0
41	60441.5
42	65025.0
43	67846.0
44	70667.0
45	76947.5
46	83228.0
47	88425.5
48	93623.0
49	97686.0
50	101749.0
51	99250.5
52	96752.0
53	96351.5
54	95951.0
55	96207.0
56	96463.0
57	93279.5
58	90096.0
59	82658.0
60	75220.0
61	65989.5
62	56759.0
63	49230.5
64	41702.0
65	35094.5
66	28487.0
67	24529.0
68	20571.0
69	18411.0
70	16251.0
71	12730.5
72	9210.0
73	8014.5
74	6819.0
75	4967.5
76	3116.0
77	2772.5
78	2429.0
79	2018.0
80	1607.0
81	1283.0
82	959.0
83	774.5
84	590.0
85	457.0
86	324.0
87	223.0
88	122.0
89	88.0
90	54.0
91	38.0
92	22.0
93	17.5
94	13.0
95	15.5
96	18.0
97	15.0
98	12.0
99	8.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03042121022368855
2	0.00447824922074603
3	5.404783542279692E-4
4	0.0
5	0.0
6	3.0884477384455377E-4
7	0.0
8	0.0
9	0.0
10	1.5442238692227689E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.404783542279692E-4
17	0.0
18	7.721119346113844E-5
19	0.0
20	7.721119346113844E-5
21	0.0
22	0.0
23	0.0
24	0.0011581679019170767
25	2.3163358038341534E-4
26	3.860559673056923E-4
27	2.3163358038341534E-4
28	0.0014670126757616304
29	3.860559673056923E-4
30	0.0010809567084559383
31	3.0884477384455377E-4
32	6.176895476891075E-4
33	2.3163358038341534E-4
34	8.49323128072523E-4
35	2.3163358038341534E-4
36	3.860559673056923E-4
37	0.0016214350626839074
38	0.0
39	8.49323128072523E-4
40	0.0
41	0.0010037455149947997
42	0.005250361155357415
43	0.004246615640362614
44	0.004401038027284892
45	0.00247075819075643
46	0.002779602964600984
47	0.0011581679019170767
48	0.004169404446901477
49	0.0037061372861346455
50	0.0016214350626839074
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1295149.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.90991357619435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.29746599597235	46.71406868633941
2	12.90171816673014	15.200782043729783
3	3.4764789233902875	6.143972187791491
4	1.4176907297857333	3.340641534777978
5	0.7327057005040084	2.1581814746738037
6	0.4410097309944877	1.5587907083487593
7	0.29918739439193803	1.233757248270109
8	0.22620209479221268	1.0660436683970695
9	0.16395732327830218	0.8692840570058046
>10	0.9136513663753868	9.770550960497495
>50	0.06838515687719773	2.815896300671536
>100	0.05548494879046334	6.440013123070146
>500	0.004876333051107922	1.8950130986326665
>1k	0.0011861350664857107	0.7930049077939223
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1404	0.10840451561943838	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	3.0884477384455377E-4
6	0.0	0.0	0.0	0.0	3.8605596730569225E-4
7	0.0	0.0	0.0	0.0	3.8605596730569225E-4
8	0.0	0.0	0.0	0.0	3.8605596730569225E-4
9	0.0	0.0	0.0	0.0	4.632671607668307E-4
10	0.0	0.0	0.0	0.0	4.632671607668307E-4
11	0.0	0.0	0.0	0.0	4.632671607668307E-4
12	0.0	0.0	0.0	0.0	6.94900741150246E-4
13	0.0	0.0	0.0	0.0	8.493231280725229E-4
14	0.0	0.0	0.0	0.0	8.493231280725229E-4
15	0.0	0.0	0.0	0.0	8.493231280725229E-4
16	0.0	0.0	0.0	0.0	8.493231280725229E-4
17	0.0	0.0	0.0	0.0	0.0010037455149947997
18	0.0	0.0	0.0	0.0	0.001389801482300492
19	0.0	0.0	0.0	7.721119346113844E-5	0.001389801482300492
20	0.0	0.0	0.0	2.3163358038341534E-4	0.001544223869222769
21	0.0	0.0	0.0	3.8605596730569225E-4	0.001544223869222769
22	0.0	0.0	0.0	5.404783542279692E-4	0.0016214350626839074
23	0.0	0.0	0.0	8.493231280725229E-4	0.0016214350626839074
24	0.0	7.721119346113844E-5	0.0	0.0014670126757616304	0.0016214350626839074
25	0.0	7.721119346113844E-5	0.0	0.001930279836528461	0.0016214350626839074
26	0.0	7.721119346113844E-5	0.0	0.0020847022234507383	0.0017758574496061844
27	0.0	7.721119346113844E-5	0.0	0.00247075819075643	0.0017758574496061844
28	0.0	7.721119346113844E-5	0.0	0.0037061372861346455	0.0017758574496061844
29	0.0	7.721119346113844E-5	0.0	0.006022473089968799	0.0017758574496061844
30	0.0	7.721119346113844E-5	0.0	0.010346299923792553	0.0017758574496061844
31	0.0	7.721119346113844E-5	0.0	0.019225587171823474	0.001930279836528461
32	0.0	7.721119346113844E-5	0.0	0.028954197547926917	0.0020074910299895995
33	0.0	7.721119346113844E-5	0.0	0.03914607508479719	0.0020074910299895995
34	0.0	7.721119346113844E-5	0.0	0.04872026307397836	0.0021619134169118767
35	0.0	7.721119346113844E-5	0.0	0.060456364480071405	0.0021619134169118767
36	0.0	7.721119346113844E-5	0.0	0.07844657255651666	0.0021619134169118767
37	0.0	7.721119346113844E-5	0.0	0.10608817981560423	0.0021619134169118767
38	0.0	7.721119346113844E-5	0.0	0.13867130345620465	0.0021619134169118767
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	820	0.0	27.91128	1
GTATCAA	1750	0.0	25.150824	1
AATTCCG	295	0.0	19.39223	42
ATTCCGA	315	0.0	18.861662	43
TGTACGC	60	0.0074120443	18.333477	2
CGCAATA	325	0.0	18.276361	36
CATATAG	230	0.0	18.174057	2
TCTAGCG	330	0.0	18.000143	28
GTCCTAA	200	3.6379788E-12	17.605577	1
ATACGAA	340	0.0	17.472075	40
TAGCGGC	340	0.0	17.47005	30
GCATATA	265	0.0	17.439486	1
TCAACGC	2585	0.0	17.105198	4
ATCAACG	2595	0.0	17.039282	3
GTCCTAC	340	0.0	16.82886	1
CAACGCA	2605	0.0	16.72053	5
CGTCGTA	290	0.0	16.688498	10
AACGCAG	2615	0.0	16.572464	6
CTAGCGG	360	0.0	16.500132	29
TATCAAC	2715	0.0	16.449486	2
>>END_MODULE