FastQCFastQC Report
Thu 2 Feb 2017
SRR4062309_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062309_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4780459
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76180.15935708265670723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC400.00528468114.2485943
GTATCAA41650.013.2916731
GGTATCA14000.013.2261271
CGTTCGG600.005872918811.08479410
CCGTGCG1408.347342E-910.8588029
TACACCG2550.010.8048935
TTACGCA803.7720858E-410.6880134
AGTATCG1701.382432E-1010.6191588
TATACCG1201.5239566E-610.2925925
ACCGTGC3250.09.9398768
GTCCTAG6300.09.6464061
GTATAAA5650.09.5797131
TAACCGT1304.237023E-69.5011536
AATCGGT1701.6711056E-89.49886319
TATCAAC58050.09.4736242
GTACTAG3550.09.3619381
CTAATAC3050.09.343343
ATCAACG58250.09.3278343
GTCTTAT5200.09.3130711
CTTATAC3600.09.2351993
Produced by FastQC (version 0.11.5)