##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062309_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4780459
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.333415473284052	32.0	32.0	32.0	32.0	32.0
2	30.989325083637365	32.0	32.0	32.0	32.0	32.0
3	31.01142630864526	32.0	32.0	32.0	32.0	32.0
4	31.015492445390706	32.0	32.0	32.0	32.0	32.0
5	30.976910585364294	32.0	32.0	32.0	32.0	32.0
6	34.59669521274003	36.0	36.0	36.0	32.0	36.0
7	34.56042463704845	36.0	36.0	36.0	32.0	36.0
8	34.53594602526661	36.0	36.0	36.0	32.0	36.0
9	34.63326973414059	36.0	36.0	36.0	32.0	36.0
10	34.41218552444441	36.0	36.0	36.0	32.0	36.0
11	34.595540721089755	36.0	36.0	36.0	32.0	36.0
12	34.49044390925641	36.0	36.0	36.0	32.0	36.0
13	34.5280480389017	36.0	36.0	36.0	32.0	36.0
14	34.47160220388879	36.0	36.0	36.0	32.0	36.0
15	34.435023498789555	36.0	36.0	36.0	32.0	36.0
16	34.435688916064336	36.0	36.0	36.0	32.0	36.0
17	34.39592808975038	36.0	36.0	36.0	32.0	36.0
18	34.387366777960025	36.0	36.0	36.0	32.0	36.0
19	34.38995502314736	36.0	36.0	36.0	32.0	36.0
20	34.36621859951105	36.0	36.0	36.0	32.0	36.0
21	34.35644171406972	36.0	36.0	36.0	32.0	36.0
22	34.32887469592355	36.0	36.0	36.0	32.0	36.0
23	34.299554917216106	36.0	36.0	36.0	32.0	36.0
24	34.27682467311193	36.0	36.0	36.0	32.0	36.0
25	33.93851301726466	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	65.0
5	375.0
6	974.0
7	217.0
8	841.0
9	639.0
10	360.0
11	85.0
12	179.0
13	204.0
14	540.0
15	875.0
16	1404.0
17	1862.0
18	2649.0
19	3548.0
20	5073.0
21	6843.0
22	10228.0
23	14942.0
24	21106.0
25	30310.0
26	43077.0
27	57019.0
28	77719.0
29	105756.0
30	142581.0
31	198657.0
32	289259.0
33	424671.0
34	981654.0
35	2356744.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57624856942217	16.297971032197857	11.563587314981913	26.56219308339806
2	16.81109518978339	18.45447604305034	37.447416038239076	27.287012728927195
3	19.836261660034072	21.25433433631103	27.931450546728833	30.977953456926066
4	13.334970725684201	14.65360155884215	34.559759730106414	37.45166798536724
5	15.362922100926518	35.469190056295815	33.63833614815909	15.529551694618574
6	36.09471406626083	33.8727979113557	15.811249215847859	14.221238806535617
7	31.422927756661956	29.843600048832453	19.275834868421466	19.45763732608413
8	28.19116421693607	33.75742651698212	18.338876804682105	19.7125324613997
9	26.918861727355146	14.791630059924406	17.94012753073448	40.34938068198596
10	15.590832418863924	27.31414522018587	31.96423002024854	25.130792340701664
11	37.27947440645228	21.115972985490416	21.63812698227165	19.966425625785654
12	24.347116117514805	23.052564795116123	28.855518604946838	23.74480048242223
13	29.761321299282095	19.657020324007863	24.620438115201495	25.961220261508544
14	23.249357268333874	18.875829017041497	25.113216271980416	32.76159744264421
15	25.09325075656274	26.894611564099215	22.013985937819424	25.998151741518623
16	25.59570246029959	26.04219002790678	23.20290272418361	25.15920478761002
17	24.24459926883698	25.77650204153869	25.03107599927533	24.947822690349003
18	24.454360446488145	25.76422624958588	25.280144043564885	24.501269260361088
19	25.317764492456234	25.20040352907872	25.15331257338986	24.328519405075184
20	25.452083957970768	24.881556180518682	25.10912660193329	24.557233259577263
21	25.482667115571683	24.511323754738733	25.128065650684057	24.877943479005523
22	25.408476339489134	24.410560287062104	25.03834111742475	25.142622256024016
23	24.950979181173324	24.643350775519192	24.893845798906405	25.511824244401076
24	24.66258960917742	24.793112956713944	25.228839839226392	25.315457594882247
25	24.888992177947507	24.657010535483977	25.249422139590976	25.204575146977536
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	258.0
1	258.0
2	677.0
3	1096.0
4	1096.0
5	1096.0
6	2324.5
7	3553.0
8	3553.0
9	3553.0
10	4704.0
11	5855.0
12	5855.0
13	5855.0
14	8229.5
15	10604.0
16	10604.0
17	10604.0
18	18209.0
19	25814.0
20	25814.0
21	25814.0
22	39916.5
23	54019.0
24	54019.0
25	54019.0
26	78581.0
27	103143.0
28	103143.0
29	103143.0
30	137768.0
31	172393.0
32	172393.0
33	172393.0
34	217937.5
35	263482.0
36	263482.0
37	263482.0
38	323237.0
39	382992.0
40	382992.0
41	382992.0
42	446826.0
43	510660.0
44	510660.0
45	510660.0
46	565856.5
47	621053.0
48	621053.0
49	621053.0
50	651659.5
51	682266.0
52	682266.0
53	682266.0
54	656114.0
55	629962.0
56	629962.0
57	629962.0
58	572123.5
59	514285.0
60	514285.0
61	514285.0
62	443051.0
63	371817.0
64	371817.0
65	371817.0
66	300814.0
67	229811.0
68	229811.0
69	229811.0
70	172325.0
71	114839.0
72	114839.0
73	114839.0
74	81415.5
75	47992.0
76	47992.0
77	47992.0
78	34435.5
79	20879.0
80	20879.0
81	20879.0
82	14646.5
83	8414.0
84	8414.0
85	8414.0
86	5435.0
87	2456.0
88	2456.0
89	2456.0
90	1570.0
91	684.0
92	684.0
93	684.0
94	493.0
95	302.0
96	302.0
97	302.0
98	1066.0
99	1830.0
100	1830.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010040876827936396
2	1.8826644052380743E-4
3	5.647993215714223E-4
4	0.010480165189158614
5	0.03338591545288852
6	0.06288099113495169
7	0.10346286831452796
8	0.10819044782101467
9	0.11735274792650664
10	0.1315354864459668
11	0.13764368651629477
12	0.1300711919085594
13	0.1256573898029457
14	0.1156165129750093
15	0.1304268062962155
16	0.11785479176790346
17	0.12287523018187166
18	0.1095501498914644
19	0.09612047713409946
20	0.09110003872013127
21	0.09189494146900958
22	0.10264704707225812
23	0.0819377386146393
24	0.08999135857037996
25	0.08825512361888262
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4780459.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.950949297110853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.40617854304105	17.458247721294455
2	15.048087340415153	9.3150517658337
3	8.14788005995257	7.565538678435975
4	5.012454270791356	6.205608719574001
5	3.3456170206157063	5.177501138631389
6	2.308258115190075	4.286566793273561
7	1.7391777107408575	3.768044080066393
8	1.3383830946076263	3.313938184104877
9	0.9923549307568774	2.764289443193466
>10	5.307167920768528	28.322426904434323
>50	0.2465301408607307	5.225809685309496
>100	0.10485955591359028	5.461067605535804
>500	0.002102004581701373	0.4276896612979518
>1k	8.8148520957491E-4	0.5483423874122142
>5k	6.780655458267935E-5	0.15987723160234393
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7618	0.15935708265670723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4642945374073913E-4
2	0.0	0.0	0.0	0.0	1.4642945374073913E-4
3	0.0	0.0	0.0	0.0	1.4642945374073913E-4
4	0.0	0.0	0.0	0.0	1.6734794713227328E-4
5	0.0	0.0	0.0	0.0	1.6734794713227328E-4
6	0.0	0.0	0.0	0.0	2.3010342730687576E-4
7	0.0	0.0	0.0	0.0	2.3010342730687576E-4
8	0.0	0.0	0.0	2.091849339153416E-5	3.137774008730124E-4
9	0.0	0.0	0.0	2.091849339153416E-5	3.3469589426454656E-4
10	0.0	0.0	0.0	2.091849339153416E-5	3.9745137443914907E-4
11	0.0	0.0	0.0	2.091849339153416E-5	3.9745137443914907E-4
12	0.0	0.0	0.0	2.091849339153416E-5	9.20413709227503E-4
13	0.0	0.0	0.0	2.091849339153416E-5	0.0010250061761851738
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	40	0.005284681	14.248594	3
GTATCAA	4165	0.0	13.291673	1
GGTATCA	1400	0.0	13.226127	1
CGTTCGG	60	0.0058729188	11.084794	10
CCGTGCG	140	8.347342E-9	10.858802	9
TACACCG	255	0.0	10.804893	5
TTACGCA	80	3.7720858E-4	10.688013	4
AGTATCG	170	1.382432E-10	10.619158	8
TATACCG	120	1.5239566E-6	10.292592	5
ACCGTGC	325	0.0	9.939876	8
GTCCTAG	630	0.0	9.646406	1
GTATAAA	565	0.0	9.579713	1
TAACCGT	130	4.237023E-6	9.501153	6
AATCGGT	170	1.6711056E-8	9.498863	19
TATCAAC	5805	0.0	9.473624	2
GTACTAG	355	0.0	9.361938	1
CTAATAC	305	0.0	9.34334	3
ATCAACG	5825	0.0	9.327834	3
GTCTTAT	520	0.0	9.313071	1
CTTATAC	360	0.0	9.235199	3
>>END_MODULE