FastQCFastQC Report
Thu 2 Feb 2017
SRR4062309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4780459
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40350.027.106761
TATACCG1600.021.9996035
GGTATCA19700.020.8900811
ATCAACG57800.018.4979363
TCAACGC57850.018.443924
CAACGCA59400.018.0737485
TATCAAC59800.018.0643522
AACGCAG60100.017.8268186
TAGGCCG1053.7786922E-516.7616025
GTATAAC3750.015.8451821
GTATAGG6350.015.2490821
AGTATCG2753.6379788E-1214.399598
ACGCAGA76050.014.0876987
GTCCTAT6550.013.7754981
GTATACG1453.8286533E-513.659641
AGAGTAC80050.013.49382311
CCTATAC4100.013.4143923
ATGCGCG1000.00876650113.19976121
TAATACC4850.013.1544024
TATAGGG6200.013.1296192
Produced by FastQC (version 0.11.5)