##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062309_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4780459
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29689533996631	32.0	32.0	32.0	32.0	32.0
2	31.44174879441493	32.0	32.0	32.0	32.0	32.0
3	31.561678700727274	32.0	32.0	32.0	32.0	32.0
4	31.645711008085208	32.0	32.0	32.0	32.0	32.0
5	31.614019281412098	32.0	32.0	32.0	32.0	32.0
6	35.26061116725403	36.0	36.0	36.0	36.0	36.0
7	35.272952450800226	36.0	36.0	36.0	36.0	36.0
8	35.219221417859664	36.0	36.0	36.0	36.0	36.0
9	35.31551489093411	36.0	36.0	36.0	36.0	36.0
10	35.188347604278164	36.0	36.0	36.0	36.0	36.0
11	35.31257542424274	36.0	36.0	36.0	36.0	36.0
12	35.23892893966877	36.0	36.0	36.0	36.0	36.0
13	35.26899467185055	36.0	36.0	36.0	36.0	36.0
14	35.23570539983713	36.0	36.0	36.0	36.0	36.0
15	35.205592182675346	36.0	36.0	36.0	36.0	36.0
16	35.22629751661922	36.0	36.0	36.0	36.0	36.0
17	35.21083352037953	36.0	36.0	36.0	36.0	36.0
18	35.20531166567896	36.0	36.0	36.0	36.0	36.0
19	35.1972030300856	36.0	36.0	36.0	36.0	36.0
20	35.19601716069524	36.0	36.0	36.0	36.0	36.0
21	35.189964185447465	36.0	36.0	36.0	36.0	36.0
22	35.16998555996401	36.0	36.0	36.0	36.0	36.0
23	35.145923226200665	36.0	36.0	36.0	36.0	36.0
24	35.12858158599415	36.0	36.0	36.0	36.0	36.0
25	35.117150884465275	36.0	36.0	36.0	36.0	36.0
26	35.075422464662914	36.0	36.0	36.0	36.0	36.0
27	35.05887468128061	36.0	36.0	36.0	36.0	36.0
28	35.04481954557083	36.0	36.0	36.0	36.0	36.0
29	35.033541130673854	36.0	36.0	36.0	36.0	36.0
30	35.009440097697734	36.0	36.0	36.0	36.0	36.0
31	35.007575632381744	36.0	36.0	36.0	36.0	36.0
32	34.98036297351363	36.0	36.0	36.0	36.0	36.0
33	34.960786401473165	36.0	36.0	36.0	36.0	36.0
34	34.955594013043516	36.0	36.0	36.0	36.0	36.0
35	34.947749787206625	36.0	36.0	36.0	36.0	36.0
36	34.91821622149672	36.0	36.0	36.0	36.0	36.0
37	34.90884912934093	36.0	36.0	36.0	36.0	36.0
38	34.90440876074871	36.0	36.0	36.0	36.0	36.0
39	34.88206571795721	36.0	36.0	36.0	32.0	36.0
40	34.87706619803663	36.0	36.0	36.0	32.0	36.0
41	34.848199304711116	36.0	36.0	36.0	32.0	36.0
42	34.83181133861832	36.0	36.0	36.0	32.0	36.0
43	34.81667388006047	36.0	36.0	36.0	32.0	36.0
44	34.78769737382959	36.0	36.0	36.0	32.0	36.0
45	34.7699482413718	36.0	36.0	36.0	32.0	36.0
46	34.752174215906884	36.0	36.0	36.0	32.0	36.0
47	34.72757950648672	36.0	36.0	36.0	32.0	36.0
48	34.70579707931812	36.0	36.0	36.0	32.0	36.0
49	34.69025819487208	36.0	36.0	36.0	32.0	36.0
50	34.27061627345826	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	33.0
21	76.0
22	246.0
23	708.0
24	1982.0
25	4647.0
26	10289.0
27	19518.0
28	34210.0
29	55095.0
30	85541.0
31	128199.0
32	197128.0
33	336673.0
34	778263.0
35	3127841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.159911211123216	16.81464464047431	11.982020419851363	27.043423728551108
2	16.21731929579106	18.922419825493716	37.05924967192995	27.80101120678528
3	19.18994587534301	21.695318804981795	28.202849692267208	30.911885627407987
4	13.08228481084549	15.051071477057528	34.586274910536794	37.280368801560186
5	15.037707699851227	35.81219030151099	33.435241324258605	15.71486067437918
6	36.30564068236254	34.001861749416896	15.623382735934902	14.069114832285665
7	31.52021594579098	29.791386140954245	19.293440232412827	19.394957680841944
8	28.33667227352018	33.75972892979524	18.23713999011392	19.666458806570667
9	26.956156302145885	14.523500776808252	17.8077460762659	40.712596844779966
10	15.509524614388251	27.302562768070658	32.03570956410853	25.152203053432558
11	37.581244813521046	20.943888442511483	21.632755348388095	19.84211139557938
12	24.40116080952093	22.900551139775967	28.86297272415587	23.83531532654723
13	29.86531209659993	19.576592958960635	24.65848990651316	25.89960503792628
14	23.3683209080969	18.62204026851815	25.132523885258717	32.87711493812623
15	25.22529740345017	26.85984755857126	21.927957127129424	25.986897910849144
16	25.66441212702877	25.966665334847562	23.153779713075963	25.21514282504771
17	24.368517751119715	25.635613651325112	25.0337258409705	24.962142756584672
18	24.66109927588498	25.623936293943505	25.26961863052395	24.445345799647562
19	25.433875701057158	25.11511969875696	25.175992514526325	24.275012085659554
20	25.644353834619682	24.736802910522194	25.05624995816292	24.562593296695205
21	25.622518674462015	24.414873132475353	25.024249763464134	24.938358429598498
22	25.466403958280992	24.318355203966817	25.04244885271477	25.172791985037417
23	25.076933021257176	24.527403969955174	24.86239286327646	25.533270145511196
24	24.795781285888626	24.649204022915168	25.17212944318212	25.382885248014087
25	25.105539205401335	24.419285962767947	25.336061388350178	25.139113443480543
26	25.020474052498503	24.602876596531576	25.156674875811667	25.219974475158253
27	24.88751021350781	24.959825823345746	25.16945114188377	24.983212821262672
28	25.290238311498957	24.632696717542657	25.001851316484753	25.07521365447364
29	24.90736212258197	24.871675045947633	25.024568857858746	25.19639397361165
30	25.166837572938487	24.887781310259104	24.871171814564395	25.074209302238014
31	25.001521826124414	24.633083014726047	25.01143722932675	25.35395792982279
32	24.97763794813008	24.92649181452676	24.937327682504883	25.158542554838277
33	25.00208139575261	24.78990454028766	24.960976444457277	25.24703761950245
34	25.087377113513686	24.7660878900383	25.145823763060548	25.000711233387467
35	25.246802440694303	24.707270853349783	25.125244668747225	24.920682037208685
36	25.332410966073716	24.749830193916768	24.935565729858656	24.982193110150856
37	25.21092992835924	24.691473207459076	25.0091886019916	25.088408262190086
38	25.13424445942209	24.76584879524096	24.94112488803374	25.158781857303214
39	25.041058099276192	24.929498887231897	24.953869125752732	25.07557388773918
40	25.28346691902774	24.82664819142625	24.916388659482408	24.9734962300636
41	25.030264096207507	24.755329922054916	25.12494202911587	25.089463952621706
42	25.371603458410995	24.71280670595348	24.977103612169287	24.938486223466235
43	25.09981695518017	24.44577166466189	25.209769363527013	25.24464201663093
44	24.903530472990415	24.83250932061103	25.06672222842829	25.197237977970268
45	25.031132572986614	24.894281041000443	25.00705490357113	25.06753148244181
46	25.21613434971425	24.773343430201912	24.825013178923765	25.18550904116008
47	24.973511747187118	25.057290979183634	25.007839267713035	24.961358005916214
48	25.092238108516824	24.967747779371592	24.8092846230491	25.130729489062485
49	25.23244502925567	24.694190913902354	24.910684961309716	25.16267909553226
50	25.141186031698215	25.046361231655546	24.752034089233213	25.06041864741303
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	57.0
1	63.5
2	70.0
3	199.0
4	328.0
5	401.5
6	475.0
7	416.5
8	358.0
9	497.5
10	637.0
11	1047.5
12	1458.0
13	2730.0
14	4002.0
15	5327.0
16	6652.0
17	6468.5
18	6285.0
19	6305.5
20	6326.0
21	7791.5
22	9257.0
23	12018.5
24	14780.0
25	18770.0
26	22760.0
27	28452.0
28	34144.0
29	42474.5
30	50805.0
31	59908.5
32	69012.0
33	80253.5
34	91495.0
35	107679.0
36	123863.0
37	139807.0
38	155751.0
39	173741.0
40	191731.0
41	213646.0
42	235561.0
43	255297.0
44	275033.0
45	296298.5
46	317564.0
47	334534.0
48	351504.0
49	361575.5
50	371647.0
51	381037.5
52	390428.0
53	393299.0
54	396170.0
55	384535.0
56	372900.0
57	351892.5
58	330885.0
59	307436.5
60	283988.0
61	251788.0
62	219588.0
63	188628.0
64	157668.0
65	132884.0
66	108100.0
67	88289.0
68	68478.0
69	56109.5
70	43741.0
71	34951.0
72	26161.0
73	21961.0
74	17761.0
75	13912.0
76	10063.0
77	8178.5
78	6294.0
79	4911.5
80	3529.0
81	2754.0
82	1979.0
83	1323.5
84	668.0
85	472.5
86	277.0
87	187.0
88	97.0
89	62.0
90	27.0
91	25.5
92	24.0
93	26.5
94	29.0
95	23.5
96	18.0
97	21.0
98	24.0
99	15.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031001207206253625
2	0.0048112534800528566
3	2.719404140899441E-4
4	4.183698678306832E-5
5	6.275548017460248E-5
6	1.8826644052380743E-4
7	0.0
8	0.0
9	0.0
10	6.275548017460248E-5
11	0.0
12	4.183698678306832E-5
13	0.0
14	0.0
15	0.0
16	6.275548017460248E-4
17	0.0
18	6.275548017460248E-5
19	0.0
20	2.3010342730687576E-4
21	0.0
22	0.0
23	4.183698678306832E-5
24	9.413322026190372E-4
25	1.6734794713227328E-4
26	3.7653288104761486E-4
27	5.22962334788354E-4
28	0.0016107239911481303
29	3.5561438765608077E-4
30	0.0012760280968835836
31	3.7653288104761486E-4
32	8.158212422698323E-4
33	2.719404140899441E-4
34	6.484732951375589E-4
35	3.3469589426454656E-4
36	1.2551096034920495E-4
37	0.0016734794713227326
38	2.091849339153416E-5
39	7.949027488782981E-4
40	1.464294537407391E-4
41	8.785767224444347E-4
42	0.004790334986661322
43	0.004371965118830639
44	0.004037269224566094
45	0.0020081753655872793
46	0.002070930845761882
47	7.949027488782981E-4
48	0.003514306889777739
49	0.003953595250999957
50	0.0013387835770581862
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4780459.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.32833859349399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.30148084909926	17.325035167622048
2	15.74222610934716	9.863555795379387
3	8.322572523343249	7.821971099406208
4	5.10605560628164	6.398569556431976
5	3.398793089831225	5.3239270363730125
6	2.351634132201919	4.420367420498347
7	1.7903968741518665	3.9263111643114037
8	1.2883560605549242	3.228964391523577
9	1.034041669702451	2.9155326793398237
>10	5.334462418886671	28.647558927839757
>50	0.23509246205338716	5.043445151448641
>100	0.09361164648632948	4.7725680308347975
>500	0.0010078091636830703	0.19985698174080102
>1k	2.6874889622240545E-4	0.11233659725020118
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.091849339153416E-4
2	0.0	0.0	0.0	0.0	2.091849339153416E-4
3	0.0	0.0	0.0	0.0	2.091849339153416E-4
4	0.0	0.0	0.0	0.0	2.510219206984099E-4
5	0.0	0.0	0.0	0.0	2.510219206984099E-4
6	0.0	0.0	0.0	0.0	3.137774008730124E-4
7	0.0	0.0	0.0	0.0	3.3469589426454656E-4
8	0.0	0.0	0.0	2.091849339153416E-5	3.3469589426454656E-4
9	0.0	0.0	0.0	2.091849339153416E-5	3.9745137443914907E-4
10	0.0	0.0	0.0	2.091849339153416E-5	4.3928836122221737E-4
11	0.0	0.0	0.0	2.091849339153416E-5	4.3928836122221737E-4
12	0.0	0.0	0.0	2.091849339153416E-5	0.0012132726167089812
13	0.0	0.0	0.0	2.091849339153416E-5	0.0013387835770581862
14	0.0	0.0	0.0	4.183698678306832E-5	0.0013597020704497205
15	0.0	0.0	0.0	1.4642945374073913E-4	0.0016316424845396645
16	0.0	0.0	0.0	2.091849339153416E-4	0.0016525609779311987
17	0.0	0.0	0.0	2.9285890748147826E-4	0.0016734794713227329
18	0.0	0.0	0.0	4.183698678306832E-4	0.0017989904316719379
19	0.0	0.0	0.0	5.22962334788354E-4	0.0017989904316719379
20	0.0	0.0	0.0	8.785767224444347E-4	0.0019245013920211427
21	0.0	0.0	0.0	0.0011295986431428446	0.001987256872195745
22	0.0	0.0	0.0	0.001840827418455006	0.0021336863259364845
23	0.0	0.0	0.0	0.0026775671541163725	0.002342871259851826
24	0.0	0.0	0.0	0.003535225383169273	0.002342871259851826
25	0.0	0.0	0.0	0.004706661013095186	0.002342871259851826
26	0.0	0.0	0.0	0.006003607603370304	0.0024474637268094967
27	0.0	0.0	0.0	0.00684034733903167	0.002489300713592565
28	0.0	0.0	0.0	0.008158212422698322	0.002489300713592565
29	0.0	0.0	0.0	0.010522002175941682	0.002489300713592565
30	0.0	0.0	0.0	0.01393171659876175	0.002489300713592565
31	0.0	0.0	0.0	0.020123590642655863	0.0025102192069840993
32	0.0	0.0	0.0	0.027424144836301283	0.0026984856475079067
33	0.0	0.0	0.0	0.03426449217533296	0.002719404140899441
34	0.0	0.0	0.0	0.04254821555838048	0.002886752088031714
35	0.0	0.0	0.0	0.05125030880925869	0.002886752088031714
36	0.0	0.0	0.0	0.06204425139929032	0.0029495075682063167
37	0.0	0.0	0.0	0.07879996460590918	0.0029495075682063167
38	0.0	0.0	0.0	0.10045060526614703	0.002991344554989385
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4035	0.0	27.10676	1
TATACCG	160	0.0	21.999603	5
GGTATCA	1970	0.0	20.890081	1
ATCAACG	5780	0.0	18.497936	3
TCAACGC	5785	0.0	18.44392	4
CAACGCA	5940	0.0	18.073748	5
TATCAAC	5980	0.0	18.064352	2
AACGCAG	6010	0.0	17.826818	6
TAGGCCG	105	3.7786922E-5	16.761602	5
GTATAAC	375	0.0	15.845182	1
GTATAGG	635	0.0	15.249082	1
AGTATCG	275	3.6379788E-12	14.39959	8
ACGCAGA	7605	0.0	14.087698	7
GTCCTAT	655	0.0	13.775498	1
GTATACG	145	3.8286533E-5	13.65964	1
AGAGTAC	8005	0.0	13.493823	11
CCTATAC	410	0.0	13.414392	3
ATGCGCG	100	0.008766501	13.199761	21
TAATACC	485	0.0	13.154402	4
TATAGGG	620	0.0	13.129619	2
>>END_MODULE