FastQCFastQC Report
Thu 2 Feb 2017
SRR4062308_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062308_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393328
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26780.11189439976467914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6050.013.1858571
GTATCAA16700.013.0796411
ATACCGT2350.011.7245536
TACTAAT658.0393243E-411.6900292
TAGACCG752.0732201E-411.4008795
CCGTCGT1701.0913936E-1111.1770979
CGTCGTA2050.011.12257610
CCGTGCG600.00587477111.0839559
ACCGTCG1900.011.0003888
CGCGCGT1658.0035534E-1110.9402387
TACCGTA700.00149095210.857987
TCAATAC1158.8324305E-710.7381613
CGCGTAA803.7703157E-410.68810110
TAGCGCG1605.4387783E-1010.6874294
TACCGTC2100.010.4055637
TCTACGA1106.062537E-610.3616172
GACGGAC2300.010.3268837
TCGTGCG750.002647228810.1339039
ACGGTAT1502.444176E-810.1339039
ACGGACC2250.010.133698
Produced by FastQC (version 0.11.5)