##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062308_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2393328
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.233343277645186	32.0	32.0	32.0	32.0	32.0
2	30.789793124887186	32.0	32.0	32.0	32.0	32.0
3	30.81233328653657	32.0	32.0	32.0	32.0	32.0
4	30.8263952955884	32.0	32.0	32.0	32.0	32.0
5	30.750890809784533	32.0	32.0	32.0	32.0	32.0
6	34.369906256058506	36.0	36.0	36.0	32.0	36.0
7	34.32392175247187	36.0	36.0	36.0	32.0	36.0
8	34.283039767219535	36.0	36.0	36.0	32.0	36.0
9	34.41341136693341	36.0	36.0	36.0	32.0	36.0
10	34.127998753200565	36.0	36.0	36.0	32.0	36.0
11	34.37965544212912	36.0	36.0	36.0	32.0	36.0
12	34.23054173936878	36.0	36.0	36.0	32.0	36.0
13	34.2954333881524	36.0	36.0	36.0	32.0	36.0
14	34.21511593897702	36.0	36.0	36.0	32.0	36.0
15	34.17102419726841	36.0	36.0	36.0	32.0	36.0
16	34.17136430944693	36.0	36.0	36.0	32.0	36.0
17	34.123523395038205	36.0	36.0	36.0	32.0	36.0
18	34.113837301030195	36.0	36.0	36.0	32.0	36.0
19	34.12549053034101	36.0	36.0	36.0	32.0	36.0
20	34.109877960730834	36.0	36.0	36.0	32.0	36.0
21	34.09759673559161	36.0	36.0	36.0	32.0	36.0
22	34.063678275606186	36.0	36.0	36.0	32.0	36.0
23	34.034796734923084	36.0	36.0	36.0	32.0	36.0
24	34.00472563727161	36.0	36.0	36.0	32.0	36.0
25	33.65033961078465	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	35.0
5	192.0
6	449.0
7	110.0
8	432.0
9	317.0
10	160.0
11	39.0
12	102.0
13	111.0
14	237.0
15	398.0
16	678.0
17	925.0
18	1307.0
19	1771.0
20	2710.0
21	3942.0
22	6050.0
23	9046.0
24	13587.0
25	19243.0
26	27437.0
27	35972.0
28	48172.0
29	65365.0
30	85313.0
31	116773.0
32	165800.0
33	233040.0
34	499153.0
35	1054460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.29006762199871	16.85189177806079	11.440502334847558	26.417538265092944
2	16.930943021600047	18.78242347058155	36.992171570298765	27.29446193751964
3	19.804713891937897	21.56457858136998	27.74100628042597	30.889701246266156
4	13.315144831759145	14.653249129680617	34.30422713424125	37.72737890431898
5	15.400430417088284	35.50401807601305	33.46219096425047	15.633360542648198
6	36.43420154298522	33.75937608310196	15.706959069877504	14.099463304035318
7	31.378965639835204	29.56070016939582	19.52326578413535	19.537068406633622
8	28.198326505089288	33.74114576471105	18.474321410570916	19.586206319628744
9	26.444674509803924	14.873139869281045	18.179346405228756	40.50283921568628
10	15.906841273387368	27.393165504044287	31.462218328685353	25.237774893882992
11	37.49116674302282	21.237429998138165	21.383113364838096	19.888289894000916
12	24.23820136158812	23.257988442440418	28.79784394993593	23.705966246035533
13	29.671061081006684	19.507208677437884	24.605345670547354	26.216384571008078
14	23.116873555833305	19.057939005760772	24.90801672514444	32.917170713261484
15	25.042871600237127	27.19316631503378	21.683511994634934	26.08045009009416
16	25.73032264919456	26.06053913048571	23.186434268556273	25.022703951763464
17	24.283810890349784	26.06748059477968	24.886036817104625	24.762671697765914
18	24.752725798875424	25.739940362235213	25.176982951496317	24.330350887393042
19	25.45426638389028	25.22683520607866	24.90404855099406	24.414849859037002
20	25.516798080936248	24.899944211317827	24.884470588825703	24.698787118920226
21	25.51763737134603	24.707542360512562	24.873316797709602	24.901503470431805
22	25.373274784362422	24.542260280819246	24.986898419989185	25.09756651482915
23	24.66770761113281	24.754559846919275	25.09469277223806	25.483039769709855
24	24.669988010209096	24.974688451535442	25.08668238261861	25.268641155636857
25	24.98731840648093	24.713571626016567	25.032315585793434	25.26679438170906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	123.0
1	123.0
2	321.5
3	520.0
4	520.0
5	520.0
6	1125.0
7	1730.0
8	1730.0
9	1730.0
10	2482.5
11	3235.0
12	3235.0
13	3235.0
14	4697.5
15	6160.0
16	6160.0
17	6160.0
18	10006.0
19	13852.0
20	13852.0
21	13852.0
22	21375.0
23	28898.0
24	28898.0
25	28898.0
26	41509.5
27	54121.0
28	54121.0
29	54121.0
30	71040.0
31	87959.0
32	87959.0
33	87959.0
34	110934.0
35	133909.0
36	133909.0
37	133909.0
38	161543.5
39	189178.0
40	189178.0
41	189178.0
42	218999.0
43	248820.0
44	248820.0
45	248820.0
46	277249.0
47	305678.0
48	305678.0
49	305678.0
50	321007.5
51	336337.0
52	336337.0
53	336337.0
54	325394.0
55	314451.0
56	314451.0
57	314451.0
58	287531.5
59	260612.0
60	260612.0
61	260612.0
62	224565.0
63	188518.0
64	188518.0
65	188518.0
66	152000.5
67	115483.0
68	115483.0
69	115483.0
70	86272.5
71	57062.0
72	57062.0
73	57062.0
74	41548.5
75	26035.0
76	26035.0
77	26035.0
78	19262.5
79	12490.0
80	12490.0
81	12490.0
82	8553.0
83	4616.0
84	4616.0
85	4616.0
86	3246.0
87	1876.0
88	1876.0
89	1876.0
90	1225.5
91	575.0
92	575.0
93	575.0
94	385.5
95	196.0
96	196.0
97	196.0
98	545.0
99	894.0
100	894.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010027877499448467
2	0.0
3	7.938736353730036E-4
4	0.010069660322362835
5	0.03196385952949199
6	0.061462532507036226
7	0.10353783518180543
8	0.10541806221295201
9	0.11293897033753834
10	0.12710334730550932
11	0.13424821002386633
12	0.12664373625345127
13	0.12305041348281556
14	0.11181083411885041
15	0.12747939271173864
16	0.1159473335873729
17	0.11962422200383734
18	0.107005809483698
19	0.0950559221301886
20	0.09033446314086493
21	0.08887206433886204
22	0.10006986087991282
23	0.07980519176644404
24	0.08887206433886204
25	0.08636509496399992
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2393328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.77691905868984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.82601802126666	33.7617020911783
2	16.19226389780636	16.12002018836106
3	6.651856150333687	9.933267155556246
4	3.182977835753088	6.337553203835408
5	1.807921434513949	4.499637945513562
6	1.102256354669157	3.2920155208975883
7	0.7305684803820286	2.545581368036439
8	0.4807251787200504	1.9143214648497724
9	0.35318444294449997	1.5822390086313154
>10	1.5598611790981076	13.213560359564752
>50	0.07339950398137843	2.483222915783003
>100	0.03652518780096433	3.346568139564468
>500	0.002189677620083581	0.7399111191883256
>1k	2.526551100091152E-4	0.2303995190398061
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2678	0.11189439976467914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.356564582873723E-5
2	0.0	0.0	0.0	4.178282291436861E-5	8.356564582873723E-5
3	0.0	0.0	0.0	4.178282291436861E-5	8.356564582873723E-5
4	0.0	0.0	0.0	8.356564582873723E-5	8.356564582873723E-5
5	0.0	0.0	0.0	8.356564582873723E-5	8.356564582873723E-5
6	0.0	0.0	0.0	8.356564582873723E-5	1.2534846874310583E-4
7	0.0	0.0	0.0	8.356564582873723E-5	1.2534846874310583E-4
8	0.0	0.0	0.0	8.356564582873723E-5	1.2534846874310583E-4
9	0.0	0.0	0.0	8.356564582873723E-5	1.2534846874310583E-4
10	0.0	0.0	0.0	8.356564582873723E-5	1.6713129165747445E-4
11	0.0	0.0	0.0	8.356564582873723E-5	2.0891411457184305E-4
12	0.0	0.0	0.0	1.2534846874310583E-4	7.103079895442664E-4
13	0.0	0.0	0.0	1.2534846874310583E-4	9.610049270304781E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	605	0.0	13.185857	1
GTATCAA	1670	0.0	13.079641	1
ATACCGT	235	0.0	11.724553	6
TACTAAT	65	8.0393243E-4	11.690029	2
TAGACCG	75	2.0732201E-4	11.400879	5
CCGTCGT	170	1.0913936E-11	11.177097	9
CGTCGTA	205	0.0	11.122576	10
CCGTGCG	60	0.005874771	11.083955	9
ACCGTCG	190	0.0	11.000388	8
CGCGCGT	165	8.0035534E-11	10.940238	7
TACCGTA	70	0.001490952	10.85798	7
TCAATAC	115	8.8324305E-7	10.738161	3
CGCGTAA	80	3.7703157E-4	10.688101	10
TAGCGCG	160	5.4387783E-10	10.687429	4
TACCGTC	210	0.0	10.405563	7
TCTACGA	110	6.062537E-6	10.361617	2
GACGGAC	230	0.0	10.326883	7
TCGTGCG	75	0.0026472288	10.133903	9
ACGGTAT	150	2.444176E-8	10.133903	9
ACGGACC	225	0.0	10.13369	8
>>END_MODULE