FastQCFastQC Report
Thu 2 Feb 2017
SRR4062308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393328
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15650.028.4041771
TCAACGC22400.018.8565544
GGTATCA7500.018.778631
ATCAACG22550.018.6335623
CAACGCA23100.018.1899075
TATCAAC24600.017.438842
AACGCAG24550.017.2947756
ACGGACC4000.015.9495018
GTATAAT3100.015.6173091
CGACGGT2750.015.1995237
GACGGAC4400.014.999537
TACGCTC2509.094947E-1214.95953134
GTATAGA3250.014.8965091
GTCTAGA4350.014.6708051
GTATAAG3150.014.6708041
CGTCGTA2701.8189894E-1214.666512510
TATATCG1057.164151E-414.6662075
CGGACCA4050.014.6662079
GTCGTGC2002.1193046E-814.2995528
TCGTAAT1100.001018591613.99985512
Produced by FastQC (version 0.11.5)