##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062308_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2393328
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24227602735605	32.0	32.0	32.0	32.0	32.0
2	31.36768173856655	32.0	32.0	32.0	32.0	32.0
3	31.521701580393493	32.0	32.0	32.0	32.0	32.0
4	31.61024899219831	32.0	32.0	32.0	32.0	32.0
5	31.57593317756697	32.0	32.0	32.0	32.0	32.0
6	35.20631355167365	36.0	36.0	36.0	36.0	36.0
7	35.21134712835015	36.0	36.0	36.0	36.0	36.0
8	35.15782082522747	36.0	36.0	36.0	36.0	36.0
9	35.26293136586377	36.0	36.0	36.0	36.0	36.0
10	35.12125124512812	36.0	36.0	36.0	36.0	36.0
11	35.253753768810626	36.0	36.0	36.0	36.0	36.0
12	35.17487699136934	36.0	36.0	36.0	36.0	36.0
13	35.210386541251346	36.0	36.0	36.0	36.0	36.0
14	35.174503452932484	36.0	36.0	36.0	36.0	36.0
15	35.148110079353934	36.0	36.0	36.0	36.0	36.0
16	35.167067363938415	36.0	36.0	36.0	36.0	36.0
17	35.145685004311986	36.0	36.0	36.0	36.0	36.0
18	35.14310449716879	36.0	36.0	36.0	36.0	36.0
19	35.13568553913212	36.0	36.0	36.0	36.0	36.0
20	35.137435403755774	36.0	36.0	36.0	36.0	36.0
21	35.12617576863681	36.0	36.0	36.0	36.0	36.0
22	35.11123464899086	36.0	36.0	36.0	36.0	36.0
23	35.07854376834266	36.0	36.0	36.0	36.0	36.0
24	35.07172606512772	36.0	36.0	36.0	36.0	36.0
25	35.05160053281456	36.0	36.0	36.0	36.0	36.0
26	35.01529585581249	36.0	36.0	36.0	36.0	36.0
27	35.000484262917574	36.0	36.0	36.0	36.0	36.0
28	34.9816101261507	36.0	36.0	36.0	36.0	36.0
29	34.96599379608645	36.0	36.0	36.0	36.0	36.0
30	34.94773177767527	36.0	36.0	36.0	36.0	36.0
31	34.95061521028459	36.0	36.0	36.0	36.0	36.0
32	34.920463889613124	36.0	36.0	36.0	32.0	36.0
33	34.900459945314644	36.0	36.0	36.0	32.0	36.0
34	34.8973195483444	36.0	36.0	36.0	32.0	36.0
35	34.88239138137355	36.0	36.0	36.0	32.0	36.0
36	34.86536905931824	36.0	36.0	36.0	32.0	36.0
37	34.855870152356886	36.0	36.0	36.0	32.0	36.0
38	34.841030564970616	36.0	36.0	36.0	32.0	36.0
39	34.83304294271408	36.0	36.0	36.0	32.0	36.0
40	34.82078219115809	36.0	36.0	36.0	32.0	36.0
41	34.79446611580193	36.0	36.0	36.0	32.0	36.0
42	34.772795454697395	36.0	36.0	36.0	32.0	36.0
43	34.7622737042311	36.0	36.0	36.0	32.0	36.0
44	34.73290664714573	36.0	36.0	36.0	32.0	36.0
45	34.72074659219296	36.0	36.0	36.0	32.0	36.0
46	34.71115158473891	36.0	36.0	36.0	32.0	36.0
47	34.682842886557964	36.0	36.0	36.0	32.0	36.0
48	34.67470651745185	36.0	36.0	36.0	32.0	36.0
49	34.65747528128196	36.0	36.0	36.0	32.0	36.0
50	34.22582278734883	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	9.0
21	28.0
22	132.0
23	380.0
24	966.0
25	2462.0
26	5235.0
27	10247.0
28	18015.0
29	29493.0
30	45926.0
31	68631.0
32	105607.0
33	182356.0
34	413595.0
35	1510241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93090304190456	17.351520116359744	11.840022068227604	26.877554773508095
2	16.39136638942339	19.23667216139027	36.63781414463031	27.73414730455602
3	19.181380182599828	22.016937970293164	28.037902145178183	30.76377970192883
4	13.081282632384697	15.090660369159597	34.362235347599665	37.46582165085604
5	15.146189740812794	35.86817185107933	33.18671740772681	15.798921000381059
6	36.59752160800493	33.90332115223752	15.589393170842586	13.909764068914972
7	31.452228862905546	29.51475936436627	19.582063135516737	19.45094863721145
8	28.21263947106289	33.82800017381654	18.38394068844722	19.57541966667335
9	26.510825093760655	14.649809804590094	18.079134995286896	40.76023010636236
10	15.818105925349943	27.369013929145495	31.561504132114	25.251376013390566
11	37.88536297573922	21.052442456696284	21.30050707633889	19.761687491225608
12	24.2639955743634	23.07172272250189	28.77023124285514	23.89405046027958
13	29.87129219229458	19.33190937472841	24.58346703836666	26.21333139461035
14	23.19711297406791	18.856211935848325	24.898509523140998	33.048165566942764
15	25.18075249192756	27.108235895790294	21.61554956111323	26.095462051168916
16	25.863178381702966	25.95585353481753	23.15153181415164	25.029436269327864
17	24.42891237640641	26.003915886163536	24.804372823114925	24.76279891431513
18	24.90230119185652	25.611231236877565	25.16507369454629	24.321393876719625
19	25.574848077655883	25.176114598584064	24.818244720322497	24.430792603437556
20	25.69656376915749	24.696154296124213	24.798773252218677	24.808508682499625
21	25.72150578608532	24.61321640828169	24.745542608451494	24.9197351971815
22	25.561602922792027	24.456697953644465	24.892451013818416	25.089248109745093
23	24.83308799842228	24.632697495996158	25.019063420919917	25.515151084661646
24	24.981061785168567	24.78848322552583	25.003582905508647	25.226872083796952
25	25.260359759213447	24.50392195286637	25.007071757552158	25.228646530368025
26	24.961434325539418	25.090376548058764	24.944762923470325	25.003426202931493
27	25.09020941620845	25.024234118295922	24.779177042769042	25.106379422726587
28	25.14203198179584	24.84290512802621	24.957349666715274	25.057713223462674
29	24.96315785735207	25.054704366613894	25.041835208762393	24.94030256727164
30	25.05169633765553	25.105889225058426	24.980790146504642	24.8616242907814
31	25.213155961723434	24.729601331707695	24.763905172651523	25.29333753391735
32	25.076442121677307	24.94194242794719	24.64707932181068	25.334536128564828
33	24.836817048743047	24.932040181738717	24.93212374745438	25.299019022063856
34	25.364452804771958	24.79323649132542	24.865855462342175	24.976455241560448
35	25.293681384684486	24.82575496432787	25.151118214199908	24.729445436787735
36	25.036549570158673	25.083095693230128	24.867412490990567	25.012942245620636
37	25.330433013175536	24.734686305388504	24.901610705557474	25.033269975878486
38	24.99065108946667	24.920288786279517	24.83116598776515	25.257894136488662
39	25.304629007141124	24.88366583504678	24.769264131305412	25.042441026506683
40	25.450941493135492	24.860257231985948	24.794658145192088	24.89414312968647
41	24.963293391306237	25.066790568010223	25.054255584961698	24.915660455721845
42	25.34562077192748	24.881896015009005	24.901618314996888	24.870864898066632
43	25.086096456444512	24.66432619128999	24.946453477828857	25.303123874436643
44	24.93871286785337	25.043048282184465	24.873529349088244	25.14470950087392
45	24.95304551294774	25.022030481244318	24.86872590905767	25.15619809675027
46	25.039945046312855	24.9544141783187	24.81590180538239	25.18973896998606
47	25.06083640022329	24.923035268398834	25.038022750139554	24.97810558123832
48	25.210113879758957	25.128633902523205	24.644851760761416	25.01640045695643
49	25.136592385590816	24.97175391475699	24.711731150752197	25.17992254889999
50	24.969780080884508	25.175522053542217	24.85683927014247	24.99785859543081
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	26.5
2	30.0
3	82.0
4	134.0
5	154.5
6	175.0
7	153.0
8	131.0
9	198.5
10	266.0
11	437.0
12	608.0
13	1059.5
14	1511.0
15	1899.5
16	2288.0
17	2502.0
18	2716.0
19	3144.0
20	3572.0
21	4522.5
22	5473.0
23	7083.5
24	8694.0
25	10973.5
26	13253.0
27	16542.5
28	19832.0
29	22985.0
30	26138.0
31	30681.0
32	35224.0
33	41503.5
34	47783.0
35	54602.5
36	61422.0
37	69356.0
38	77290.0
39	86747.5
40	96205.0
41	106428.0
42	116651.0
43	125386.0
44	134121.0
45	144948.5
46	155776.0
47	163803.0
48	171830.0
49	179712.5
50	187595.0
51	191193.0
52	194791.0
53	195442.0
54	196093.0
55	191437.0
56	186781.0
57	177324.0
58	167867.0
59	155976.5
60	144086.0
61	126842.5
62	109599.0
63	93894.5
64	78190.0
65	65667.0
66	53144.0
67	44002.0
68	34860.0
69	29449.0
70	24038.0
71	18798.5
72	13559.0
73	11407.0
74	9255.0
75	7060.5
76	4866.0
77	4010.0
78	3154.0
79	2631.5
80	2109.0
81	1618.0
82	1127.0
83	851.5
84	576.0
85	446.5
86	317.0
87	206.5
88	96.0
89	62.5
90	29.0
91	21.0
92	13.0
93	13.0
94	13.0
95	12.0
96	11.0
97	9.5
98	8.0
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03125355153994772
2	0.005139287218467339
3	2.924797604005803E-4
4	0.0
5	0.0
6	8.356564582873723E-5
7	0.0
8	0.0
9	0.0
10	4.178282291436861E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.192221041161094E-4
17	0.0
18	1.2534846874310583E-4
19	0.0
20	3.342625833149489E-4
21	0.0
22	0.0
23	4.178282291436861E-5
24	7.938736353730036E-4
25	2.0891411457184305E-4
26	3.342625833149489E-4
27	3.342625833149489E-4
28	0.0012117018645166897
29	3.760454062293175E-4
30	0.0010445705728592152
31	4.178282291436861E-4
32	5.849595208011606E-4
33	8.356564582873723E-5
34	5.849595208011606E-4
35	1.2534846874310583E-4
36	1.2534846874310583E-4
37	0.0012117018645166897
38	4.178282291436861E-5
39	2.924797604005803E-4
40	8.356564582873723E-5
41	0.001086353395773584
42	0.004094716645608124
43	0.0034261914789782263
44	0.0035515399477213322
45	0.0022144896144615364
46	0.0016713129165747444
47	0.0010027877499448467
48	0.0042618479372655985
49	0.0031754945414920145
50	0.0016295300936603758
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2393328.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.13074191271202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.56271876927353	33.36838475630884
2	17.203277331117008	17.248261118780718
3	6.853022724091737	10.306413405101805
4	3.2827035282739336	6.582574534073989
5	1.8183961301924703	4.557877354887651
6	1.1180436826712992	3.3629015581879798
7	0.7226771935889256	2.5359840714606614
8	0.488061322697536	1.9573500964581632
9	0.34298092048099527	1.5474499205055459
>10	1.5076813143709484	12.801179721126072
>50	0.06863655953455013	2.334226687058166
>100	0.030041427339074136	2.7773316114044144
>500	0.0016753298742984	0.5698838107415817
>1k	8.376649371471552E-5	0.050181353904275354
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.356564582873723E-5
2	0.0	0.0	0.0	0.0	8.356564582873723E-5
3	0.0	0.0	0.0	0.0	8.356564582873723E-5
4	0.0	0.0	0.0	0.0	8.356564582873723E-5
5	0.0	0.0	0.0	0.0	1.2534846874310583E-4
6	0.0	0.0	0.0	0.0	1.2534846874310583E-4
7	0.0	0.0	0.0	0.0	1.2534846874310583E-4
8	0.0	0.0	0.0	0.0	1.2534846874310583E-4
9	0.0	0.0	0.0	0.0	1.2534846874310583E-4
10	0.0	0.0	0.0	4.178282291436861E-5	1.2534846874310583E-4
11	0.0	0.0	0.0	4.178282291436861E-5	1.2534846874310583E-4
12	0.0	0.0	0.0	1.6713129165747445E-4	7.520908124586351E-4
13	0.0	0.0	0.0	1.6713129165747445E-4	0.0010027877499448467
14	0.0	0.0	0.0	1.6713129165747445E-4	0.0010027877499448467
15	0.0	0.0	0.0	2.5069693748621167E-4	0.0013370503332597956
16	0.0	0.0	0.0	2.5069693748621167E-4	0.0014206159790885328
17	0.0	0.0	0.0	2.924797604005803E-4	0.0014623988020029013
18	0.0	0.0	0.0	4.596110520580547E-4	0.0015459644478316387
19	0.0	0.0	0.0	5.013938749724233E-4	0.0015459644478316387
20	0.0	0.0	0.0	5.013938749724233E-4	0.0017966613853178503
21	0.0	0.0	0.0	6.267423437155291E-4	0.0020055754998896933
22	0.0	0.0	0.0	6.267423437155291E-4	0.002047358322804062
23	0.0	0.0	0.0	7.520908124586351E-4	0.002047358322804062
24	0.0	0.0	0.0	0.0010863533957735838	0.002047358322804062
25	0.0	0.0	0.0	0.0013370503332597956	0.002047358322804062
26	0.0	0.0	0.0	0.0016713129165747444	0.0021727067915471676
27	0.0	0.0	0.0	0.0022980552602902735	0.0022144896144615364
28	0.0	0.0	0.0	0.0028830147810914343	0.002256272437375905
29	0.0	0.0	0.0	0.00451254487475181	0.002256272437375905
30	0.0	0.0	0.0	0.007562690947500719	0.002256272437375905
31	0.0	0.0	0.0	0.013286937686769218	0.0023816209061190106
32	0.0	0.0	0.0	0.019972189353068198	0.0023816209061190106
33	0.0	0.0	0.0	0.025780001738165432	0.0023816209061190106
34	0.0	0.0	0.0	0.03233990493572131	0.0025487521977764853
35	0.0	0.0	0.0	0.03877445966453407	0.0025487521977764853
36	0.0	0.0	0.0	0.049554427976441176	0.0025487521977764853
37	0.0	0.0	0.0	0.06513942092350067	0.0025487521977764853
38	0.0	0.0	0.0	0.08786927658891719	0.0027158834894339596
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1565	0.0	28.404177	1
TCAACGC	2240	0.0	18.856554	4
GGTATCA	750	0.0	18.77863	1
ATCAACG	2255	0.0	18.633562	3
CAACGCA	2310	0.0	18.189907	5
TATCAAC	2460	0.0	17.43884	2
AACGCAG	2455	0.0	17.294775	6
ACGGACC	400	0.0	15.949501	8
GTATAAT	310	0.0	15.617309	1
CGACGGT	275	0.0	15.199523	7
GACGGAC	440	0.0	14.99953	7
TACGCTC	250	9.094947E-12	14.959531	34
GTATAGA	325	0.0	14.896509	1
GTCTAGA	435	0.0	14.670805	1
GTATAAG	315	0.0	14.670804	1
CGTCGTA	270	1.8189894E-12	14.6665125	10
TATATCG	105	7.164151E-4	14.666207	5
CGGACCA	405	0.0	14.666207	9
GTCGTGC	200	2.1193046E-8	14.299552	8
TCGTAAT	110	0.0010185916	13.999855	12
>>END_MODULE