FastQCFastQC Report
Thu 2 Feb 2017
SRR4062307_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062307_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997773
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18790.18831938727546244No Hit
TATCAACGCAGAGTACTTTTTTTTT13130.13159305773958607No Hit
GCGCAAGACGGACCAGAGCGAAAGC11420.1144548910423513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG250.00603031719.000155
CGTCTTA350.002169350916.28584315
TAAACCT551.1274136E-515.5455784
AACCGCG456.758911E-414.7778967
TAGCACC456.758911E-414.7778964
CGCGTAA1101.8553692E-1013.81829210
GCGTAAC1101.8553692E-1013.81829211
GTAGCAC707.2557013E-613.5715353
CGAGCCG2050.012.97571215
CCGAGCC2300.012.8044514
GTCGGCC604.0894645E-412.66676612
TCGCGTA1155.313268E-912.3914039
CGCTATT853.938956E-612.29421511
ACGCTAT853.938956E-612.29421510
ATACGCT853.938956E-612.2942158
TATACGC853.938956E-612.2942157
CGACCAT2400.012.2709310
GGTATCA6400.012.1707511
GACCATA2350.012.12775511
GTTATAG550.00306924212.0897911
Produced by FastQC (version 0.11.5)