##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062307_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	997773
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.222194827881694	32.0	32.0	32.0	32.0	32.0
2	30.766695430724223	32.0	32.0	32.0	32.0	32.0
3	30.79298397531302	32.0	32.0	32.0	32.0	32.0
4	30.7941505733268	32.0	32.0	32.0	32.0	32.0
5	30.722329628081738	32.0	32.0	32.0	32.0	32.0
6	34.383385800176995	36.0	36.0	36.0	32.0	36.0
7	34.3218637906618	36.0	36.0	36.0	32.0	36.0
8	34.26797077090681	36.0	36.0	36.0	32.0	36.0
9	34.399514719279836	36.0	36.0	36.0	32.0	36.0
10	34.084653523396604	36.0	36.0	36.0	32.0	36.0
11	34.37931272944848	36.0	36.0	36.0	32.0	36.0
12	34.2021201215106	36.0	36.0	36.0	32.0	36.0
13	34.28683778775333	36.0	36.0	36.0	32.0	36.0
14	34.18694833393968	36.0	36.0	36.0	32.0	36.0
15	34.144986885794665	36.0	36.0	36.0	32.0	36.0
16	34.13831202087048	36.0	36.0	36.0	32.0	36.0
17	34.08064259105027	36.0	36.0	36.0	32.0	36.0
18	34.08783560990325	36.0	36.0	36.0	32.0	36.0
19	34.090382281340545	36.0	36.0	36.0	32.0	36.0
20	34.0678721512809	36.0	36.0	36.0	32.0	36.0
21	34.05541841681425	36.0	36.0	36.0	32.0	36.0
22	34.0220140252342	36.0	36.0	36.0	32.0	36.0
23	33.990009751717075	36.0	36.0	36.0	32.0	36.0
24	33.967757195273876	36.0	36.0	36.0	32.0	36.0
25	33.613800934681535	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	12.0
5	74.0
6	203.0
7	38.0
8	170.0
9	128.0
10	81.0
11	23.0
12	53.0
13	37.0
14	215.0
15	279.0
16	432.0
17	548.0
18	753.0
19	1034.0
20	1535.0
21	2171.0
22	3346.0
23	4920.0
24	6860.0
25	9159.0
26	12240.0
27	15445.0
28	19903.0
29	25997.0
30	33568.0
31	45374.0
32	63432.0
33	89910.0
34	199284.0
35	460545.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.98568603692044	17.029526933642824	11.298629840433689	25.686157189003044
2	17.18450425999553	18.900729726560503	37.200519958988586	26.71424605445538
3	20.10693900868441	21.949757708478447	27.49735659198308	30.445946690854058
4	13.341084727414875	15.00997323764371	33.888460112061104	37.76048192288031
5	15.46997107638941	35.78336867327348	32.738018256462695	16.00864199387441
6	35.99073349780372	33.60230258539423	15.992839521030147	14.414124395771907
7	31.728410544533347	29.45947979633299	19.029321026361334	19.78278863277233
8	28.69531191210776	33.05300122908672	18.250984523540772	20.000702335264755
9	26.725382746624092	14.80110822670682	18.212387373601043	40.261121653068045
10	17.324398537139555	26.2631422595654	29.750783818611726	26.66167538468332
11	37.54746201783959	21.206275474921284	21.16402010191474	20.082242405324376
12	24.979626901321943	23.31686089689807	27.57955544493643	24.123956756843555
13	29.82635372938559	19.151468561926276	24.583245939840495	26.438931768847638
14	23.70830824804335	19.388922335942205	24.212422235601043	32.690347180413404
15	25.456565439624573	26.78012097466196	21.53407192041003	26.229241665303437
16	26.129830825162585	25.639034141025522	23.072060111806515	25.15907492200537
17	24.44451580402963	26.008361113396848	24.40889176111219	25.13823132146133
18	25.07605402042823	24.981739008287683	25.36863122830253	24.573575742981557
19	25.996689406099517	24.762740770465488	24.586677367576243	24.65389245585875
20	26.105907502726083	24.292196125599514	24.343256916028576	25.258639455645827
21	26.622079013841766	24.063488751432025	24.15337296531633	25.161059269409876
22	26.38216969490233	24.05395643755079	24.41624110842455	25.14763275912233
23	24.842367328760666	24.241126328818844	25.06585163771468	25.85065470470581
24	25.04037637806334	24.519345551576436	24.975874487144765	25.464403583215468
25	25.167868714327273	24.39538172954429	24.79652125066455	25.640228305463886
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	401.0
1	401.0
2	402.0
3	403.0
4	403.0
5	403.0
6	738.0
7	1073.0
8	1073.0
9	1073.0
10	1301.5
11	1530.0
12	1530.0
13	1530.0
14	1895.5
15	2261.0
16	2261.0
17	2261.0
18	3676.5
19	5092.0
20	5092.0
21	5092.0
22	7909.0
23	10726.0
24	10726.0
25	10726.0
26	16372.0
27	22018.0
28	22018.0
29	22018.0
30	28482.5
31	34947.0
32	34947.0
33	34947.0
34	44090.5
35	53234.0
36	53234.0
37	53234.0
38	63854.5
39	74475.0
40	74475.0
41	74475.0
42	87537.0
43	100599.0
44	100599.0
45	100599.0
46	113667.0
47	126735.0
48	126735.0
49	126735.0
50	133205.0
51	139675.0
52	139675.0
53	139675.0
54	132768.0
55	125861.0
56	125861.0
57	125861.0
58	117027.0
59	108193.0
60	108193.0
61	108193.0
62	95760.5
63	83328.0
64	83328.0
65	83328.0
66	67980.0
67	52632.0
68	52632.0
69	52632.0
70	40173.0
71	27714.0
72	27714.0
73	27714.0
74	20698.5
75	13683.0
76	13683.0
77	13683.0
78	10765.0
79	7847.0
80	7847.0
81	7847.0
82	5422.5
83	2998.0
84	2998.0
85	2998.0
86	2207.5
87	1417.0
88	1417.0
89	1417.0
90	937.0
91	457.0
92	457.0
93	457.0
94	276.0
95	95.0
96	95.0
97	95.0
98	237.0
99	379.0
100	379.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.013391823591137E-4
2	2.0044639411970458E-4
3	8.017855764788183E-4
4	0.010322989297164787
5	0.031870976665033024
6	0.0634412837388865
7	0.10503391051872521
8	0.1100450703717178
9	0.1228736395953789
10	0.1372055567749378
11	0.14532363573678583
12	0.13640377119845895
13	0.13249506651312473
14	0.11756181015120674
15	0.13690488718375823
16	0.12327453238361832
17	0.12578011231011463
18	0.11154841832761561
19	0.09751717073923628
20	0.09240578768918381
21	0.09370868925096189
22	0.10453279453342594
23	0.08368636954497667
24	0.09050154694504661
25	0.08649261906265251
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	997773.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.079471921348976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.67633683674909	42.99968478668366
2	13.790775741213304	15.467588419059844
3	4.219144733970192	7.098222259223663
4	1.7807792117871064	3.99460631222148
5	0.9382433761879442	2.6308096535161742
6	0.5517892970200567	1.85664314332423
7	0.3740486989206688	1.4683517455837136
8	0.2763023850087874	1.2395913473521638
9	0.20635623298063355	1.041511371590951
>10	1.0443471827279787	10.414099391789403
>50	0.07776964144887584	3.0352543135547205
>100	0.05979697065151831	6.768797434954578
>500	0.0037709799170594625	1.5483966052730929
>1k	5.387114167227803E-4	0.4364432158723715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1879	0.18831938727546244	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1313	0.13159305773958607	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1142	0.1144548910423513	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.0022319705985229E-4
7	0.0	0.0	0.0	0.0	1.0022319705985229E-4
8	0.0	0.0	0.0	0.0	1.0022319705985229E-4
9	0.0	0.0	0.0	0.0	2.0044639411970458E-4
10	0.0	0.0	0.0	0.0	2.0044639411970458E-4
11	0.0	0.0	0.0	0.0	2.0044639411970458E-4
12	0.0	0.0	0.0	0.0	5.011159852992615E-4
13	0.0	0.0	0.0	0.0	6.013391823591137E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	25	0.006030317	19.00015	5
CGTCTTA	35	0.0021693509	16.285843	15
TAAACCT	55	1.1274136E-5	15.545578	4
AACCGCG	45	6.758911E-4	14.777896	7
TAGCACC	45	6.758911E-4	14.777896	4
CGCGTAA	110	1.8553692E-10	13.818292	10
GCGTAAC	110	1.8553692E-10	13.818292	11
GTAGCAC	70	7.2557013E-6	13.571535	3
CGAGCCG	205	0.0	12.975712	15
CCGAGCC	230	0.0	12.80445	14
GTCGGCC	60	4.0894645E-4	12.666766	12
TCGCGTA	115	5.313268E-9	12.391403	9
CGCTATT	85	3.938956E-6	12.294215	11
ACGCTAT	85	3.938956E-6	12.294215	10
ATACGCT	85	3.938956E-6	12.294215	8
TATACGC	85	3.938956E-6	12.294215	7
CGACCAT	240	0.0	12.27093	10
GGTATCA	640	0.0	12.170751	1
GACCATA	235	0.0	12.127755	11
GTTATAG	55	0.003069242	12.089791	1
>>END_MODULE