FastQCFastQC Report
Thu 2 Feb 2017
SRR4062307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997773
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12340.12367542517185773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.025.9458831
GTATCAA14200.024.9490321
TAAACGC1700.020.7061928
CGTCTTA808.9890054E-519.2493215
GTAAACG1851.8189894E-1219.0273127
GTATAGG708.110217E-418.8612021
ATACCGT2850.018.5256616
CGCAATA2050.018.24325836
CTTAGAC1456.3064363E-918.2062533
ACCGTCG2950.017.8976738
TAAGACT1509.59335E-917.599384
CCGTCGT2750.017.5993779
CGTCGTA2900.017.4476610
TACCGTC2950.017.1519377
TAGGACG2201.8189894E-1216.9994014
CGAGCCG3000.016.86607215
GTCCTAA1702.4592737E-916.8271521
CTAGATA1702.4665496E-916.8229353
CGCGGGA2109.094947E-1216.76299344
TACGAAT2109.094947E-1216.76215441
Produced by FastQC (version 0.11.5)