##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062307_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	997773
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24809651092984	32.0	32.0	32.0	32.0	32.0
2	31.465757241376547	32.0	32.0	32.0	32.0	32.0
3	31.574862218159844	32.0	32.0	32.0	32.0	32.0
4	31.65511794766946	32.0	32.0	32.0	32.0	32.0
5	31.63319713000853	32.0	32.0	32.0	32.0	32.0
6	35.28100880661233	36.0	36.0	36.0	36.0	36.0
7	35.286886897119885	36.0	36.0	36.0	36.0	36.0
8	35.23720826280126	36.0	36.0	36.0	36.0	36.0
9	35.30376247904083	36.0	36.0	36.0	36.0	36.0
10	35.20680756043709	36.0	36.0	36.0	36.0	36.0
11	35.31449437898199	36.0	36.0	36.0	36.0	36.0
12	35.24383502059086	36.0	36.0	36.0	36.0	36.0
13	35.27332168739783	36.0	36.0	36.0	36.0	36.0
14	35.233112140737425	36.0	36.0	36.0	36.0	36.0
15	35.219841587214724	36.0	36.0	36.0	36.0	36.0
16	35.225197514865606	36.0	36.0	36.0	36.0	36.0
17	35.20624129937371	36.0	36.0	36.0	36.0	36.0
18	35.20545254281284	36.0	36.0	36.0	36.0	36.0
19	35.191507487173936	36.0	36.0	36.0	36.0	36.0
20	35.19216795804256	36.0	36.0	36.0	36.0	36.0
21	35.18150922103525	36.0	36.0	36.0	36.0	36.0
22	35.170454602399545	36.0	36.0	36.0	36.0	36.0
23	35.13620833596419	36.0	36.0	36.0	36.0	36.0
24	35.125040465115816	36.0	36.0	36.0	36.0	36.0
25	35.10240806275576	36.0	36.0	36.0	36.0	36.0
26	35.07247339825792	36.0	36.0	36.0	36.0	36.0
27	35.05352319615784	36.0	36.0	36.0	36.0	36.0
28	35.03455996504215	36.0	36.0	36.0	36.0	36.0
29	35.015883372270046	36.0	36.0	36.0	36.0	36.0
30	34.98874493497018	36.0	36.0	36.0	36.0	36.0
31	34.99529151420213	36.0	36.0	36.0	36.0	36.0
32	34.96877045179615	36.0	36.0	36.0	36.0	36.0
33	34.94662814086972	36.0	36.0	36.0	36.0	36.0
34	34.93966964429785	36.0	36.0	36.0	36.0	36.0
35	34.92710867101034	36.0	36.0	36.0	36.0	36.0
36	34.902775480996176	36.0	36.0	36.0	36.0	36.0
37	34.8887612713513	36.0	36.0	36.0	32.0	36.0
38	34.87631054358055	36.0	36.0	36.0	32.0	36.0
39	34.85530977486863	36.0	36.0	36.0	32.0	36.0
40	34.850836813583854	36.0	36.0	36.0	32.0	36.0
41	34.8242636351154	36.0	36.0	36.0	32.0	36.0
42	34.79022182400205	36.0	36.0	36.0	32.0	36.0
43	34.78308392790745	36.0	36.0	36.0	32.0	36.0
44	34.73742825271881	36.0	36.0	36.0	32.0	36.0
45	34.722802681571864	36.0	36.0	36.0	32.0	36.0
46	34.69551491170837	36.0	36.0	36.0	32.0	36.0
47	34.671672815359805	36.0	36.0	36.0	32.0	36.0
48	34.6349229734619	36.0	36.0	36.0	32.0	36.0
49	34.625615245150954	36.0	36.0	36.0	32.0	36.0
50	34.23025076846136	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	9.0
21	43.0
22	137.0
23	245.0
24	551.0
25	1310.0
26	2657.0
27	4851.0
28	8187.0
29	12660.0
30	18445.0
31	26751.0
32	39939.0
33	67258.0
34	152504.0
35	662219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.55828893732293	17.512647098335577	11.808570151043348	26.12049381329815
2	16.6502292221681	19.266589290403537	36.829297138882374	27.253884348545988
3	19.571483551286473	22.258581153133793	27.87982777559513	30.290107519984605
4	13.12823658287005	15.331543347033843	33.950006664842604	37.5902134052535
5	15.136809675146552	36.114426828547174	32.58596895285801	16.162794543448257
6	36.12408446017727	33.7862758225326	15.860136383863246	14.229503333426873
7	31.844217071418047	29.491677966832135	19.077385337145824	19.586719624603994
8	28.664034805511875	33.06202913889231	18.35437519355605	19.919560862039763
9	27.097746681860503	14.436349750895245	18.279007349367042	40.18689621787721
10	17.440540082764315	26.249457541945915	29.85578884175058	26.45421353353919
11	37.84748635210614	21.037951518030653	21.161727166399572	19.952834963463633
12	24.98935128531239	23.22752770419725	27.71862938764629	24.064491622844074
13	30.03288323095534	18.982874862318383	24.665029019626708	26.319212887099567
14	23.77063720906459	19.098432208528394	24.347922824129338	32.783007758277684
15	25.60752796477756	26.702466392656444	21.575147854271464	26.114857788294533
16	26.23926604180531	25.49560619302283	23.14546066821145	25.119667096960413
17	24.61722255462916	25.836337523665204	24.48803485361901	25.058405068086632
18	25.25707275810506	24.71095600999026	25.449100596128172	24.58287063577651
19	26.165871395597996	24.531531721142986	24.749617397945222	24.552979485313795
20	26.298519399742027	24.00159956543135	24.572973157167326	25.126907877659306
21	26.748669286501038	23.84279791094768	24.36646411558541	25.042068686965873
22	26.450805944839157	23.801205284167843	24.714138386186036	25.033850384806968
23	24.940642811541302	23.99172958177862	25.224274459220688	25.843353147459393
24	25.09583939272152	24.333333667414006	25.15176449718019	25.419062442684286
25	25.301071992687678	23.989845334787248	25.10593645015675	25.603146222368327
26	24.863570626066757	24.982235367104007	25.40257314067685	24.751620866152386
27	25.46816854769281	24.3726265846572	24.874846031804893	25.2843588358451
28	25.159558048260294	24.29231194801134	25.21107364926896	25.33705635445941
29	24.920171983523257	24.51937821341592	25.105785902562715	25.454663900498108
30	24.905488672672092	24.612934521590276	25.30007095866357	25.18150584707406
31	25.525747943662306	24.273353852444803	24.624637566410662	25.576260637482225
32	25.470603209983945	24.44911712412379	24.39780228150601	25.682477384386253
33	24.92598494643054	24.2806458402237	25.04605269751546	25.747316515830303
34	25.577889850145525	24.41319024061706	25.0505127444456	24.958407164791815
35	25.82746942935804	24.282625973294472	25.249380869959136	24.64052372738835
36	24.76267123150379	24.649118235428535	24.98376382580389	25.604446707263783
37	25.74577052597819	24.28159076330981	24.923528704297627	25.049110006414367
38	25.072135646083826	24.34371344985282	24.9304200454412	25.653730858622154
39	25.808979834009676	24.145969658307685	24.58865729442386	25.45639321325878
40	25.95800848489586	24.312744481961328	24.82638836689307	24.902858666249738
41	24.95026403907117	24.64758877054709	25.393252345983893	25.00889484439785
42	25.96501074934727	24.710268758738543	24.795862628854355	24.52885786305983
43	25.53155237146425	23.990767115214613	24.909144484824676	25.568536028496457
44	24.993309846803008	24.56904889574887	24.8274341383233	25.610207119124816
45	25.184613534316867	24.64239897370109	24.80265795381655	25.370329538165493
46	25.07479320992913	24.497394640546588	24.811601291304143	25.61621085822014
47	25.32755643351604	24.40269120947075	25.180628343090124	25.08912401392309
48	25.72666508309254	24.93888706811273	24.16383778187369	25.17061006692104
49	25.321475892565417	24.984414808637904	24.332041750272115	25.36206754852457
50	25.29215442591405	25.15204057087877	24.450469050673508	25.105335952533675
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	187.0
1	125.0
2	63.0
3	148.5
4	234.0
5	238.5
6	243.0
7	190.5
8	138.0
9	192.5
10	247.0
11	421.0
12	595.0
13	1106.0
14	1617.0
15	2013.5
16	2410.0
17	2325.0
18	2240.0
19	2067.5
20	1895.0
21	2061.0
22	2227.0
23	2575.0
24	2923.0
25	3583.5
26	4244.0
27	5516.5
28	6789.0
29	8225.0
30	9661.0
31	11267.0
32	12873.0
33	15247.0
34	17621.0
35	20600.5
36	23580.0
37	27358.5
38	31137.0
39	34697.0
40	38257.0
41	41983.5
42	45710.0
43	48807.5
44	51905.0
45	57223.5
46	62542.0
47	66968.5
48	71395.0
49	75374.0
50	79353.0
51	78354.5
52	77356.0
53	78285.5
54	79215.0
55	78696.0
56	78177.0
57	76222.0
58	74267.0
59	68647.0
60	63027.0
61	55532.0
62	48037.0
63	41537.0
64	35037.0
65	29611.5
66	24186.0
67	20577.5
68	16969.0
69	14662.5
70	12356.0
71	9619.5
72	6883.0
73	5919.0
74	4955.0
75	3726.0
76	2497.0
77	2130.0
78	1763.0
79	1529.5
80	1296.0
81	1032.0
82	768.0
83	625.5
84	483.0
85	371.5
86	260.0
87	170.5
88	81.0
89	55.0
90	29.0
91	22.5
92	16.0
93	14.5
94	13.0
95	10.0
96	7.0
97	6.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03116941428561406
2	0.005111383050052467
3	5.011159852992615E-4
4	0.0
5	0.0
6	1.0022319705985229E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.011159852992615E-4
17	0.0
18	1.0022319705985229E-4
19	0.0
20	2.0044639411970458E-4
21	0.0
22	0.0
23	0.0
24	0.001002231970598523
25	5.011159852992615E-4
26	4.0089278823940915E-4
27	2.0044639411970458E-4
28	0.001703794350017489
29	3.0066959117955685E-4
30	9.020087735386707E-4
31	4.0089278823940915E-4
32	0.0011024551676583753
33	3.0066959117955685E-4
34	5.011159852992615E-4
35	2.0044639411970458E-4
36	1.0022319705985229E-4
37	0.0013029015617780798
38	0.0
39	4.0089278823940915E-4
40	0.0
41	4.0089278823940915E-4
42	0.0038084814882743873
43	0.00350781189709483
44	0.0038084814882743873
45	0.0013029015617780798
46	0.0022049103353167505
47	6.013391823591137E-4
48	0.0030066959117955685
49	0.003106919108855421
50	0.0013029015617780798
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	997773.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.571222944077796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25058052638911	43.89839171100105
2	14.297909430270861	16.46296262888712
3	4.331474545234487	7.481048601608779
4	1.7309316431277793	3.986074061098731
5	0.9032742941171839	2.600130288313744
6	0.5502780025510137	1.900810653965173
7	0.37851333911811885	1.525403308357358
8	0.2642998758934959	1.2172853663325236
9	0.20554043968059166	1.0649893029187603
>10	0.9636038621015471	9.804758724848712
>50	0.07038263945139325	2.7883074045022944
>100	0.04901050228362382	5.517527132202194
>500	0.004025862290034451	1.6279591254178833
>1k	1.750374908710631E-4	0.12435169054569285
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1234	0.12367542517185773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.0022319705985229E-4	0.0	0.0	0.0
6	0.0	1.0022319705985229E-4	0.0	0.0	0.0
7	0.0	1.0022319705985229E-4	0.0	1.0022319705985229E-4	0.0
8	1.0022319705985229E-4	1.0022319705985229E-4	0.0	1.0022319705985229E-4	0.0
9	1.0022319705985229E-4	1.0022319705985229E-4	0.0	1.0022319705985229E-4	0.0
10	1.0022319705985229E-4	1.0022319705985229E-4	0.0	1.0022319705985229E-4	0.0
11	1.0022319705985229E-4	1.0022319705985229E-4	0.0	1.0022319705985229E-4	0.0
12	1.0022319705985229E-4	1.0022319705985229E-4	0.0	1.0022319705985229E-4	1.0022319705985229E-4
13	1.0022319705985229E-4	1.0022319705985229E-4	0.0	2.0044639411970458E-4	3.0066959117955685E-4
14	1.0022319705985229E-4	1.0022319705985229E-4	0.0	2.0044639411970458E-4	3.0066959117955685E-4
15	1.0022319705985229E-4	1.0022319705985229E-4	0.0	2.0044639411970458E-4	4.0089278823940915E-4
16	1.0022319705985229E-4	1.0022319705985229E-4	0.0	3.0066959117955685E-4	4.0089278823940915E-4
17	1.0022319705985229E-4	1.0022319705985229E-4	0.0	3.0066959117955685E-4	4.0089278823940915E-4
18	1.0022319705985229E-4	1.0022319705985229E-4	0.0	5.011159852992615E-4	4.0089278823940915E-4
19	1.0022319705985229E-4	1.0022319705985229E-4	0.0	5.011159852992615E-4	4.0089278823940915E-4
20	1.0022319705985229E-4	1.0022319705985229E-4	0.0	8.017855764788183E-4	6.013391823591137E-4
21	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.001002231970598523	7.015623794189661E-4
22	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.0011024551676583753	7.015623794189661E-4
23	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.0016035711529576366	7.015623794189661E-4
24	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.0016035711529576366	7.015623794189661E-4
25	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.0019042407441371935	7.015623794189661E-4
26	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.0028062495176758642	8.017855764788183E-4
27	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.0033073655029751256	8.017855764788183E-4
28	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.004510043867693353	8.017855764788183E-4
29	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.006915400597129808	8.017855764788183E-4
30	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.012127006844242127	8.017855764788183E-4
31	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.02275066573258647	9.020087735386707E-4
32	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.033975663803289924	0.0011024551676583753
33	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.043997983509275154	0.0011024551676583753
34	1.0022319705985229E-4	1.0022319705985229E-4	0.0	0.05472186559467935	0.0012026783647182274
35	1.0022319705985229E-4	2.0044639411970458E-4	0.0	0.06724976522716089	0.0012026783647182274
36	1.0022319705985229E-4	2.0044639411970458E-4	0.0	0.085490387092054	0.0012026783647182274
37	1.0022319705985229E-4	2.0044639411970458E-4	0.0	0.11124774873643604	0.0012026783647182274
38	1.0022319705985229E-4	2.0044639411970458E-4	0.0	0.1485307780427011	0.0012026783647182274
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	670	0.0	25.945883	1
GTATCAA	1420	0.0	24.949032	1
TAAACGC	170	0.0	20.70619	28
CGTCTTA	80	8.9890054E-5	19.24932	15
GTAAACG	185	1.8189894E-12	19.02731	27
GTATAGG	70	8.110217E-4	18.861202	1
ATACCGT	285	0.0	18.525661	6
CGCAATA	205	0.0	18.243258	36
CTTAGAC	145	6.3064363E-9	18.206253	3
ACCGTCG	295	0.0	17.897673	8
TAAGACT	150	9.59335E-9	17.59938	4
CCGTCGT	275	0.0	17.599377	9
CGTCGTA	290	0.0	17.44766	10
TACCGTC	295	0.0	17.151937	7
TAGGACG	220	1.8189894E-12	16.999401	4
CGAGCCG	300	0.0	16.866072	15
GTCCTAA	170	2.4592737E-9	16.827152	1
CTAGATA	170	2.4665496E-9	16.822935	3
CGCGGGA	210	9.094947E-12	16.762993	44
TACGAAT	210	9.094947E-12	16.762154	41
>>END_MODULE