FastQCFastQC Report
Thu 2 Feb 2017
SRR4062306_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062306_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294841
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27900.21547047089179291No Hit
TATCAACGCAGAGTACTTTTTTTTT16710.12905059385669745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCTA653.3671495E-614.6170215
GGTATCA5550.014.2038531
TTTATAC551.9591754E-413.818663
ATCGCCA1650.013.81705716
CGTGCAA707.2547937E-613.5724229
AGTGTCG500.001498045413.3014895
CCCCTAA500.0015019813.296861
CGCATCG1800.013.19490113
CGCCAGT1750.013.02751118
TTACGCG604.0873376E-412.6680855
GTATCAA15200.012.6220211
ACTCTAA2000.012.35090410
CAAGACG2550.012.2950184
TACGCGA701.08909444E-412.21612556
GCCGCTA1405.2750693E-1112.2147078
GTATTAA1103.8236976E-812.0880561
ACGGTAT2550.011.9224429
AAGACGG2400.011.8763295
GCGTTAT802.8724206E-511.8721961
GACTTAA802.8724206E-511.8721961
Produced by FastQC (version 0.11.5)