##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062306_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1294841
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23160372586287	32.0	32.0	32.0	32.0	32.0
2	30.880691142773514	32.0	32.0	32.0	32.0	32.0
3	30.8945940080674	32.0	32.0	32.0	32.0	32.0
4	30.89286097675313	32.0	32.0	32.0	32.0	32.0
5	30.86166641309628	32.0	32.0	32.0	32.0	32.0
6	34.45523195512036	36.0	36.0	36.0	32.0	36.0
7	34.39786274917152	36.0	36.0	36.0	32.0	36.0
8	34.39079238300301	36.0	36.0	36.0	32.0	36.0
9	34.47160076024778	36.0	36.0	36.0	32.0	36.0
10	34.26450969655734	36.0	36.0	36.0	32.0	36.0
11	34.439157394614476	36.0	36.0	36.0	32.0	36.0
12	34.3294752019746	36.0	36.0	36.0	32.0	36.0
13	34.38143293269212	36.0	36.0	36.0	32.0	36.0
14	34.313226102664345	36.0	36.0	36.0	32.0	36.0
15	34.28728855511989	36.0	36.0	36.0	32.0	36.0
16	34.2788296014723	36.0	36.0	36.0	32.0	36.0
17	34.22456888529171	36.0	36.0	36.0	32.0	36.0
18	34.222411863696	36.0	36.0	36.0	32.0	36.0
19	34.226423939309925	36.0	36.0	36.0	32.0	36.0
20	34.210080619937116	36.0	36.0	36.0	32.0	36.0
21	34.18863474357083	36.0	36.0	36.0	32.0	36.0
22	34.16803993694979	36.0	36.0	36.0	32.0	36.0
23	34.13166172526202	36.0	36.0	36.0	32.0	36.0
24	34.11334519064503	36.0	36.0	36.0	32.0	36.0
25	33.772634632360266	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	18.0
5	115.0
6	256.0
7	59.0
8	242.0
9	215.0
10	87.0
11	35.0
12	61.0
13	57.0
14	224.0
15	372.0
16	535.0
17	735.0
18	962.0
19	1272.0
20	1856.0
21	2580.0
22	3595.0
23	5120.0
24	7207.0
25	9953.0
26	13715.0
27	17828.0
28	23468.0
29	31479.0
30	41604.0
31	56464.0
32	80747.0
33	115726.0
34	262957.0
35	615293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.334037676162936	17.071200410248803	11.604048117736465	25.990713795851793
2	16.75618901249966	19.28755401267343	37.18697749134059	26.769279483486315
3	19.169914838307083	22.68921581271996	28.209781817086554	29.9310875318864
4	12.983097897490598	15.324892194353712	35.04040732499106	36.65160258316463
5	15.036113340806567	36.11596751228564	33.36155754378293	15.486361603124854
6	34.995610469339745	34.718559986645936	16.373567606500213	13.912261937514103
7	30.754492610639378	30.163080877042958	20.018154461373832	19.06427205094383
8	28.290156321760872	32.94230986316241	19.01955993622802	19.7479738788487
9	27.079974482398654	14.514025014981923	18.483713197626088	39.92228730499333
10	16.119806530148846	26.845411278110593	31.29005164661281	25.74473054512775
11	37.05224241346106	21.452144959258728	21.832525726749243	19.66308690053097
12	24.26205875688213	23.61466114678548	28.556104570452344	23.56717552588005
13	29.288312700681157	19.63481442986445	24.999303995991017	26.07756887346338
14	23.350522678259228	19.4524153620008	25.09041224271077	32.10664971702921
15	25.14730778065042	27.0594667918496	22.11917487535198	25.674050552147996
16	25.65425229193215	26.023172949633167	23.429902755267424	24.892672003167256
17	23.98755580439135	26.091747716592185	25.018230916696115	24.90246556232035
18	24.589601141253294	25.415308722159725	25.698611707124712	24.296478429462272
19	25.410702937888573	25.199418019441616	25.42299503522962	23.966884007440196
20	25.4870175048006	24.709722106265673	24.923236764719526	24.8800236242142
21	26.10278372591007	24.51912893575244	24.786910845012716	24.591176493324777
22	25.684201248486556	24.377341685441383	25.071864518952285	24.866592547119776
23	24.432894623948478	24.48615193871312	25.430174558772034	25.650778878566367
24	24.514916604308006	25.030148609453367	25.34972386965906	25.105210916579573
25	24.701367067472145	24.59306842294447	25.3698657668835	25.33569874269989
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	341.0
1	341.0
2	423.0
3	505.0
4	505.0
5	505.0
6	1010.5
7	1516.0
8	1516.0
9	1516.0
10	1840.5
11	2165.0
12	2165.0
13	2165.0
14	3013.0
15	3861.0
16	3861.0
17	3861.0
18	6281.5
19	8702.0
20	8702.0
21	8702.0
22	13199.0
23	17696.0
24	17696.0
25	17696.0
26	25480.5
27	33265.0
28	33265.0
29	33265.0
30	41970.5
31	50676.0
32	50676.0
33	50676.0
34	63426.5
35	76177.0
36	76177.0
37	76177.0
38	90288.5
39	104400.0
40	104400.0
41	104400.0
42	120504.0
43	136608.0
44	136608.0
45	136608.0
46	152132.5
47	167657.0
48	167657.0
49	167657.0
50	173927.0
51	180197.0
52	180197.0
53	180197.0
54	169597.5
55	158998.0
56	158998.0
57	158998.0
58	145408.0
59	131818.0
60	131818.0
61	131818.0
62	115281.0
63	98744.0
64	98744.0
65	98744.0
66	79799.0
67	60854.0
68	60854.0
69	60854.0
70	46020.0
71	31186.0
72	31186.0
73	31186.0
74	23232.0
75	15278.0
76	15278.0
77	15278.0
78	11833.5
79	8389.0
80	8389.0
81	8389.0
82	5818.0
83	3247.0
84	3247.0
85	3247.0
86	2349.0
87	1451.0
88	1451.0
89	1451.0
90	964.5
91	478.0
92	478.0
93	478.0
94	298.5
95	119.0
96	119.0
97	119.0
98	316.0
99	513.0
100	513.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013129025108102076
2	4.6337735675654385E-4
3	7.722955945942398E-4
4	0.011584433918913596
5	0.034289924399984247
6	0.06587681421888865
7	0.11545819139183884
8	0.11453143667832576
9	0.12480296808642916
10	0.14078948689452989
11	0.14874413151885058
12	0.1404033390972328
13	0.13476558125669483
14	0.12341283601615952
15	0.13901320702696315
16	0.12426236117021319
17	0.13190808755669614
18	0.11785230773508099
19	0.10248362540265563
20	0.09676863800265824
21	0.0966141788837394
22	0.11082441782427341
23	0.0866515657134737
24	0.09645971976482055
25	0.09236655311347107
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1294841.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.488475153897845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62584865254132	45.98705995368296
2	12.920073582135794	15.113508053905628
3	3.724518311420013	6.53524190153181
4	1.5559150810334106	3.6401240223439038
5	0.8108585105592023	2.371293892408426
6	0.4987674371665544	1.7503288113773596
7	0.3276237639180062	1.3413550063021358
8	0.24913288753826246	1.1657122162240394
9	0.1859501663361035	0.9788347515251128
>10	0.9748462572490293	10.227228339415204
>50	0.07032998318597945	2.8579669698222308
>100	0.051756030193768676	5.987653552916029
>500	0.0038485080289005474	1.5225625765265511
>1k	5.308286936414548E-4	0.5211299520187227
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2790	0.21547047089179291	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1671	0.12905059385669745	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.722955945942398E-5	0.0
8	0.0	0.0	0.0	2.3168867837827193E-4	0.0
9	0.0	0.0	0.0	3.089182378376959E-4	0.0
10	0.0	0.0	0.0	3.8614779729711986E-4	0.0
11	0.0	0.0	0.0	3.8614779729711986E-4	0.0
12	0.0	0.0	0.0	3.8614779729711986E-4	7.722955945942398E-5
13	0.0	0.0	0.0	3.8614779729711986E-4	2.3168867837827193E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCCTA	65	3.3671495E-6	14.617021	5
GGTATCA	555	0.0	14.203853	1
TTTATAC	55	1.9591754E-4	13.81866	3
ATCGCCA	165	0.0	13.817057	16
CGTGCAA	70	7.2547937E-6	13.572422	9
AGTGTCG	50	0.0014980454	13.301489	5
CCCCTAA	50	0.00150198	13.29686	1
CGCATCG	180	0.0	13.194901	13
CGCCAGT	175	0.0	13.027511	18
TTACGCG	60	4.0873376E-4	12.668085	5
GTATCAA	1520	0.0	12.622021	1
ACTCTAA	200	0.0	12.350904	10
CAAGACG	255	0.0	12.295018	4
TACGCGA	70	1.08909444E-4	12.2161255	6
GCCGCTA	140	5.2750693E-11	12.214707	8
GTATTAA	110	3.8236976E-8	12.088056	1
ACGGTAT	255	0.0	11.922442	9
AAGACGG	240	0.0	11.876329	5
GCGTTAT	80	2.8724206E-5	11.872196	1
GACTTAA	80	2.8724206E-5	11.872196	1
>>END_MODULE