FastQCFastQC Report
Thu 2 Feb 2017
SRR4062306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294841
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14900.024.6651211
GGTATCA8100.022.5499781
TATTCGC500.002580089322.00065644
GTATCGT550.004485010619.99905210
TATACCG808.9924724E-519.2490885
TACACCG1051.7937018E-618.8562495
CGCAATA1901.8189894E-1218.52686936
CTAGCGG1901.8189894E-1218.52615429
CGGATCG1002.4834222E-517.59984426
ATACGAA2055.456968E-1217.17190740
GTGTAGG2450.017.0663491
GCGTAAC1551.4400939E-817.03145211
CGAATGC2201.8189894E-1217.00050743
TCAACGC21550.016.843754
ATCAACG21650.016.765953
TATAACA2650.016.6029854
ATAACGC3450.016.5789243
CAACGCA21950.016.5368025
CGCGTAA1602.1307642E-816.49921810
TGTACTA1206.4101296E-616.4992185
Produced by FastQC (version 0.11.5)