##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062306_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1294841
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248549435799454	32.0	32.0	32.0	32.0	32.0
2	31.388829979897146	32.0	32.0	32.0	32.0	32.0
3	31.529773153615	32.0	32.0	32.0	32.0	32.0
4	31.619812007806363	32.0	32.0	32.0	32.0	32.0
5	31.587222678305675	32.0	32.0	32.0	32.0	32.0
6	35.21223918612401	36.0	36.0	36.0	36.0	36.0
7	35.21827699308255	36.0	36.0	36.0	36.0	36.0
8	35.16749160707763	36.0	36.0	36.0	36.0	36.0
9	35.25508305652972	36.0	36.0	36.0	36.0	36.0
10	35.12398279016497	36.0	36.0	36.0	36.0	36.0
11	35.25696668548493	36.0	36.0	36.0	36.0	36.0
12	35.172271344512566	36.0	36.0	36.0	36.0	36.0
13	35.211218211347955	36.0	36.0	36.0	36.0	36.0
14	35.17599149239173	36.0	36.0	36.0	36.0	36.0
15	35.15237546540463	36.0	36.0	36.0	36.0	36.0
16	35.163915878474654	36.0	36.0	36.0	36.0	36.0
17	35.14486257386042	36.0	36.0	36.0	36.0	36.0
18	35.13896764158688	36.0	36.0	36.0	36.0	36.0
19	35.13693959335548	36.0	36.0	36.0	36.0	36.0
20	35.12631743974743	36.0	36.0	36.0	36.0	36.0
21	35.12303209428802	36.0	36.0	36.0	36.0	36.0
22	35.107583865509355	36.0	36.0	36.0	36.0	36.0
23	35.07619622795386	36.0	36.0	36.0	36.0	36.0
24	35.0592752314763	36.0	36.0	36.0	36.0	36.0
25	35.04569055196738	36.0	36.0	36.0	36.0	36.0
26	34.996940164854216	36.0	36.0	36.0	36.0	36.0
27	34.99008063538303	36.0	36.0	36.0	36.0	36.0
28	34.97148066828282	36.0	36.0	36.0	36.0	36.0
29	34.95246134467475	36.0	36.0	36.0	36.0	36.0
30	34.9281672421556	36.0	36.0	36.0	32.0	36.0
31	34.92685820112276	36.0	36.0	36.0	32.0	36.0
32	34.90512734768207	36.0	36.0	36.0	32.0	36.0
33	34.88371699691314	36.0	36.0	36.0	32.0	36.0
34	34.874824013141385	36.0	36.0	36.0	32.0	36.0
35	34.86512938654244	36.0	36.0	36.0	32.0	36.0
36	34.842207653294885	36.0	36.0	36.0	32.0	36.0
37	34.82882068145819	36.0	36.0	36.0	32.0	36.0
38	34.81289285711527	36.0	36.0	36.0	32.0	36.0
39	34.79096584059356	36.0	36.0	36.0	32.0	36.0
40	34.78103334695148	36.0	36.0	36.0	32.0	36.0
41	34.74902323914674	36.0	36.0	36.0	32.0	36.0
42	34.72027916941153	36.0	36.0	36.0	32.0	36.0
43	34.72023283167586	36.0	36.0	36.0	32.0	36.0
44	34.67705224039091	36.0	36.0	36.0	32.0	36.0
45	34.65598401657037	36.0	36.0	36.0	32.0	36.0
46	34.63586803321798	36.0	36.0	36.0	32.0	36.0
47	34.607849149046096	36.0	36.0	36.0	32.0	36.0
48	34.58939360122208	36.0	36.0	36.0	32.0	36.0
49	34.573943055556626	36.0	36.0	36.0	32.0	36.0
50	34.14591830193823	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	8.0
21	19.0
22	88.0
23	244.0
24	639.0
25	1505.0
26	3219.0
27	5996.0
28	10478.0
29	16885.0
30	25503.0
31	38115.0
32	58055.0
33	97435.0
34	219680.0
35	816969.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93390674513354	17.66895279472589	12.05045447030995	26.346685989830622
2	16.246159597029934	19.763034200246217	36.746999118000964	27.24380708472288
3	18.637775536399147	23.08790752202206	28.499704210101957	29.774612731476836
4	12.673370707291474	15.650415765333351	35.21505729274869	36.46115623462649
5	14.7694581805797	36.49336096092107	33.164535259541516	15.57264559895771
6	35.26525650639731	34.781645005062394	16.207318118595257	13.745780369945034
7	30.83297485946151	30.172816585202355	19.963146054225962	19.031062501110174
8	28.378773918959933	33.06792108065778	18.910816077031853	19.642488923350435
9	27.248905464068564	14.176644082169162	18.32889134650509	40.245559107257186
10	16.106314293657903	26.7756634024281	31.3389299063977	25.779092397516294
11	37.387447570782825	21.207391486676745	21.79109249707107	19.614068445469364
12	24.427033455870994	23.513098143399958	28.491782768527386	23.56808563220166
13	29.408089487435134	19.416129084574862	25.072730937620914	26.10305049036909
14	23.53794790248378	19.200349695445233	25.031258664191203	32.23044373787979
15	25.185254405753295	26.88832065095251	22.08062611548445	25.845798827809745
16	25.786204808669567	25.907919816377156	23.431142978063495	24.87473239688978
17	24.197256651588884	25.866187431507033	25.017511802607423	24.91904411429666
18	24.808779463099686	25.23462358283649	25.678153285348	24.278443668715827
19	25.561825737677445	25.07867761369929	25.357321864228886	24.002174784394377
20	25.722968563562155	24.471303293469823	24.9828742658331	24.822853877134925
21	26.312208458187886	24.385715609650614	24.722591208180162	24.57948472398134
22	25.848888010188126	24.20428454149969	25.072113101145238	24.87471434716695
23	24.612211074564367	24.27827045946182	25.5004282379072	25.609090228066613
24	24.692854958341957	24.729848288730242	25.451565767808543	25.12573098511926
25	24.929354151365537	24.384421538121728	25.418140788159764	25.268083522352974
26	24.688010441484824	25.071070831418673	25.472048562172013	24.76887016492449
27	25.12835602346552	24.835809322570583	25.054910428811063	24.980924225152837
28	24.8453076393158	24.63485497687724	25.470873351518502	25.048964032288456
29	24.83766283915492	24.97806672041865	25.32961703258173	24.8546534078447
30	24.81794780900217	25.01975176491577	25.405133219186283	24.75716720689578
31	25.082076221412336	24.72882603393642	24.903752066868854	25.285345677782384
32	25.113567031965584	24.755682213109058	24.82619339990578	25.30455735501958
33	24.748945621810897	24.521504217898904	25.251633600779712	25.47791655951049
34	25.22116820058865	24.613520395357224	25.4350960628778	24.730215341176326
35	25.613319967439942	24.48669254377768	25.384951631014847	24.515035857767536
36	24.80959007999456	24.889754548445442	25.138125387114062	25.162529984445932
37	25.480994983070953	24.760197587919826	25.035294590119367	24.72351283888985
38	24.903366513726397	24.546797637702237	25.332685634761333	25.217150213810037
39	25.280905384563702	24.62128262298323	24.967505411903172	25.130306580549895
40	25.604844470771226	24.683338520601435	25.122312692640076	24.589504315987263
41	24.699939219397894	25.050026219840426	25.35261630758228	24.897418253179396
42	25.587579483781177	24.975034002414347	25.054662597962096	24.382723915842384
43	25.20223481636291	24.35027672612069	25.21806759747973	25.229420860036672
44	24.88245107276912	24.85688688425833	25.14697477563756	25.11368726733499
45	25.035468277805624	25.054235379616763	24.978858048885595	24.931438293692015
46	24.973220029795854	24.792575904900406	25.028517675552646	25.205686389751094
47	25.016102549686213	24.82294918390579	25.33892584795177	24.822022418456225
48	25.452180716275567	25.26952434427135	24.581608781716753	24.696686157736327
49	25.0094803091469	25.22634962144411	24.722097577288654	25.042072492120337
50	25.149828701000608	25.324294183248224	24.731544038825714	24.794333076925454
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	196.0
1	122.5
2	49.0
3	149.0
4	249.0
5	279.0
6	309.0
7	238.5
8	168.0
9	240.0
10	312.0
11	529.0
12	746.0
13	1279.0
14	1812.0
15	2335.5
16	2859.0
17	2819.0
18	2779.0
19	2702.5
20	2626.0
21	3097.0
22	3568.0
23	4297.0
24	5026.0
25	6333.0
26	7640.0
27	9498.0
28	11356.0
29	13203.0
30	15050.0
31	17314.5
32	19579.0
33	22260.0
34	24941.0
35	29146.5
36	33352.0
37	38366.0
38	43380.0
39	48740.5
40	54101.0
41	59371.0
42	64641.0
43	68460.5
44	72280.0
45	78621.0
46	84962.0
47	90030.0
48	95098.0
49	98822.5
50	102547.0
51	101365.0
52	100183.0
53	99291.5
54	98400.0
55	98432.5
56	98465.0
57	94581.0
58	90697.0
59	82798.0
60	74899.0
61	65628.0
62	56357.0
63	48693.0
64	41029.0
65	34659.5
66	28290.0
67	23938.5
68	19587.0
69	17042.0
70	14497.0
71	11413.0
72	8329.0
73	7197.5
74	6066.0
75	4496.5
76	2927.0
77	2551.0
78	2175.0
79	1844.5
80	1514.0
81	1147.0
82	780.0
83	661.5
84	543.0
85	416.5
86	290.0
87	197.0
88	104.0
89	69.5
90	35.0
91	26.0
92	17.0
93	15.0
94	13.0
95	10.0
96	7.0
97	7.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0283432483216086
2	0.004247625770268318
3	2.3168867837827193E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	7.722955945942398E-5
11	0.0
12	7.722955945942398E-5
13	0.0
14	0.0
15	0.0
16	0.0010039842729725116
17	0.0
18	7.722955945942398E-5
19	0.0
20	4.6337735675654385E-4
21	7.722955945942398E-5
22	0.0
23	0.0
24	0.0010039842729725116
25	1.5445911891884796E-4
26	4.6337735675654385E-4
27	3.861477972971199E-4
28	0.0010039842729725116
29	3.861477972971199E-4
30	0.0015445911891884797
31	6.178364756753918E-4
32	8.495251540536638E-4
33	1.5445911891884796E-4
34	9.267547135130877E-4
35	2.3168867837827193E-4
36	2.3168867837827193E-4
37	0.0019307389864855996
38	0.0
39	7.722955945942398E-4
40	3.089182378376959E-4
41	0.0015445911891884797
42	0.006178364756753919
43	0.004556544008106014
44	0.004711003127024863
45	0.0016990503081073275
46	0.0023168867837827195
47	0.0011584433918913597
48	0.004633773567565439
49	0.004015937091890047
50	0.002239657224323295
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1294841.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.975146486642416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.53902805221868	47.10389712350334
2	13.180607220281177	15.810176976385208
3	3.739611657536749	6.728512708917665
4	1.4963059186611072	3.589646666421199
5	0.8104483139446018	2.4303378174339927
6	0.4763010151935268	1.7139733894780957
7	0.3167357860610548	1.3297392616601518
8	0.22923564092438134	1.0998752915519308
9	0.18009811873786274	0.9721269947944833
>10	0.9202107030580877	9.810657272768715
>50	0.06303524675314534	2.6178188940602283
>100	0.044750426327405696	5.22459540716832
>500	0.003242768126947783	1.315499877600484
>1k	3.89132175233734E-4	0.2531423182561783
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.722955945942398E-5
2	0.0	0.0	0.0	0.0	7.722955945942398E-5
3	0.0	0.0	0.0	0.0	7.722955945942398E-5
4	0.0	0.0	0.0	0.0	7.722955945942398E-5
5	0.0	0.0	0.0	0.0	7.722955945942398E-5
6	0.0	0.0	0.0	0.0	7.722955945942398E-5
7	0.0	0.0	0.0	7.722955945942398E-5	7.722955945942398E-5
8	0.0	0.0	0.0	1.5445911891884796E-4	7.722955945942398E-5
9	0.0	0.0	0.0	1.5445911891884796E-4	7.722955945942398E-5
10	0.0	0.0	0.0	1.5445911891884796E-4	7.722955945942398E-5
11	0.0	0.0	0.0	1.5445911891884796E-4	7.722955945942398E-5
12	0.0	0.0	0.0	1.5445911891884796E-4	2.3168867837827193E-4
13	0.0	0.0	0.0	1.5445911891884796E-4	2.3168867837827193E-4
14	0.0	0.0	0.0	1.5445911891884796E-4	3.089182378376959E-4
15	0.0	0.0	0.0	2.3168867837827193E-4	3.089182378376959E-4
16	0.0	0.0	0.0	2.3168867837827193E-4	3.089182378376959E-4
17	0.0	0.0	0.0	2.3168867837827193E-4	3.089182378376959E-4
18	0.0	0.0	0.0	2.3168867837827193E-4	3.089182378376959E-4
19	0.0	0.0	0.0	2.3168867837827193E-4	3.089182378376959E-4
20	0.0	0.0	0.0	2.3168867837827193E-4	3.8614779729711986E-4
21	0.0	0.0	0.0	3.089182378376959E-4	3.8614779729711986E-4
22	0.0	0.0	0.0	5.406069162159678E-4	3.8614779729711986E-4
23	0.0	0.0	0.0	7.722955945942397E-4	4.6337735675654385E-4
24	0.0	0.0	0.0	0.0010812138324319356	4.6337735675654385E-4
25	0.0	0.0	0.0	0.0012356729513507837	4.6337735675654385E-4
26	0.0	0.0	0.0	0.0012356729513507837	6.178364756753918E-4
27	0.0	0.0	0.0	0.0013129025108102076	6.178364756753918E-4
28	0.0	0.0	0.0	0.0020851981054044472	6.178364756753918E-4
29	0.0	0.0	0.0	0.0038614779729711987	6.178364756753918E-4
30	0.0	0.0	0.0	0.007491267267564126	6.178364756753918E-4
31	0.0	0.0	0.0	0.01552314145134422	6.178364756753918E-4
32	0.0	0.0	0.0	0.023632245194583738	6.178364756753918E-4
33	0.0	0.0	0.0	0.03135520114052613	6.178364756753918E-4
34	0.0	0.0	0.0	0.04000491179998162	6.950660351348158E-4
35	0.0	0.0	0.0	0.05035367276754443	6.950660351348158E-4
36	0.0	0.0	0.0	0.0641005343513219	6.950660351348158E-4
37	0.0	0.0	0.0	0.08811892734320276	6.950660351348158E-4
38	0.0	0.0	0.0	0.11298684548913727	6.950660351348158E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1490	0.0	24.665121	1
GGTATCA	810	0.0	22.549978	1
TATTCGC	50	0.0025800893	22.000656	44
GTATCGT	55	0.0044850106	19.999052	10
TATACCG	80	8.9924724E-5	19.249088	5
TACACCG	105	1.7937018E-6	18.856249	5
CGCAATA	190	1.8189894E-12	18.526869	36
CTAGCGG	190	1.8189894E-12	18.526154	29
CGGATCG	100	2.4834222E-5	17.599844	26
ATACGAA	205	5.456968E-12	17.171907	40
GTGTAGG	245	0.0	17.066349	1
GCGTAAC	155	1.4400939E-8	17.031452	11
CGAATGC	220	1.8189894E-12	17.000507	43
TCAACGC	2155	0.0	16.84375	4
ATCAACG	2165	0.0	16.76595	3
TATAACA	265	0.0	16.602985	4
ATAACGC	345	0.0	16.578924	3
CAACGCA	2195	0.0	16.536802	5
CGCGTAA	160	2.1307642E-8	16.499218	10
TGTACTA	120	6.4101296E-6	16.499218	5
>>END_MODULE