FastQCFastQC Report
Thu 2 Feb 2017
SRR4062305_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062305_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5979349
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80630.13484745580162658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.014.7666041
GTATCAA51650.012.6680691
GCGTAAC3250.012.27881711
CGCGTAA3200.011.87663410
TCTAACG658.044854E-411.6896082
TCGCGTA3350.011.34474859
CGAACGA1354.7402864E-911.25920516
CGATATA600.005872958311.08485810
ACGAACG1553.1286618E-1011.03294415
ACCGTCG4850.010.9698468
CGTCGTA4550.010.85863610
TAATACG909.532298E-510.5547094
GTGTACG3600.010.2878271
TAGGTCG750.002648642510.1337095
GTCTTAG5250.010.1295531
AAGACGG7950.010.0381085
TCTTATA4650.010.0084412
ATACCGT6550.010.0084236
CCGTCGT4750.010.0012919
CCGACCA7250.09.8288559
Produced by FastQC (version 0.11.5)