##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062305_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5979349
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.308317845303897	32.0	32.0	32.0	32.0	32.0
2	31.01054496066378	32.0	32.0	32.0	32.0	32.0
3	31.019374349950137	32.0	32.0	32.0	32.0	32.0
4	31.02622442677288	32.0	32.0	32.0	32.0	32.0
5	30.97820247655723	32.0	32.0	32.0	32.0	32.0
6	34.528044273716084	36.0	36.0	36.0	32.0	36.0
7	34.50006480638611	36.0	36.0	36.0	32.0	36.0
8	34.49941339767925	36.0	36.0	36.0	32.0	36.0
9	34.60935864422699	36.0	36.0	36.0	32.0	36.0
10	34.41999304606572	36.0	36.0	36.0	32.0	36.0
11	34.53490873337549	36.0	36.0	36.0	32.0	36.0
12	34.462235270093785	36.0	36.0	36.0	32.0	36.0
13	34.50296344969996	36.0	36.0	36.0	32.0	36.0
14	34.45040304554894	36.0	36.0	36.0	32.0	36.0
15	34.41237315299709	36.0	36.0	36.0	32.0	36.0
16	34.401708948582865	36.0	36.0	36.0	32.0	36.0
17	34.363402604531025	36.0	36.0	36.0	32.0	36.0
18	34.3548289287011	36.0	36.0	36.0	32.0	36.0
19	34.35442370064032	36.0	36.0	36.0	32.0	36.0
20	34.34025995137598	36.0	36.0	36.0	32.0	36.0
21	34.322991181815944	36.0	36.0	36.0	32.0	36.0
22	34.292363600117675	36.0	36.0	36.0	32.0	36.0
23	34.26937213398984	36.0	36.0	36.0	32.0	36.0
24	34.24300053400462	36.0	36.0	36.0	32.0	36.0
25	33.865632195076756	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	78.0
5	476.0
6	1305.0
7	234.0
8	1022.0
9	863.0
10	483.0
11	100.0
12	219.0
13	259.0
14	762.0
15	1155.0
16	2112.0
17	2941.0
18	4030.0
19	5207.0
20	7025.0
21	9458.0
22	12908.0
23	17930.0
24	25038.0
25	35482.0
26	50186.0
27	67616.0
28	92936.0
29	129296.0
30	178303.0
31	255685.0
32	383091.0
33	578382.0
34	1353650.0
35	2761114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.288985574386274	16.13546413080472	11.414088115739398	26.161462179069606
2	17.31733512974179	17.746195314598374	37.0660263728192	27.870443182840642
3	20.409328508915333	20.63017357368457	26.99537822539576	31.96511969200434
4	13.509522338436033	14.024514566719928	33.18672474142088	39.27923835342316
5	15.774635068857268	35.18672000631724	32.81303640511456	16.22560851971093
6	37.60041756839708	32.821475118265305	15.094816408469985	14.483290904867637
7	32.71813799680161	28.881176787307165	18.38040397735948	20.02028123853174
8	29.060183797738777	33.32305909889215	17.433629059396907	20.18312804397216
9	26.596501720942722	14.832054877276926	17.51957534675037	41.051868055029985
10	16.20425974589697	26.755775327743613	30.503969229511963	26.535995696847458
11	38.263945295686995	21.004201969181093	20.685026486306338	20.046826248825575
12	24.97687318803689	22.66092643898675	27.71176523388726	24.6504351390891
13	29.817649004265938	18.94603318136719	24.492671715459103	26.74364609890777
14	23.44639536801462	18.47092911793195	24.25675839718973	33.8259171168637
15	25.452593125625167	26.712175640546647	21.331280667941293	26.503950565886893
16	26.291835352088572	25.816429627997366	22.415500450255703	25.476234569658356
17	24.77038380608454	25.866731012226886	24.121982839025694	25.24090234266288
18	25.30681253674057	25.18321520819754	24.658068877968315	24.851903377093574
19	25.797186462758624	24.81055840677046	24.54804797942639	24.844207151044525
20	25.98621342623259	24.34333486837403	24.420639316828215	25.249812388565168
21	26.14322235569358	24.04377704301572	24.463910505445767	25.349090095844932
22	26.089531089411388	23.813074624197007	24.525011371735115	25.572382914656487
23	25.10121572691873	24.14600171934504	24.879384731573282	25.873397822162946
24	25.071545366525207	24.416237349828386	24.681159429994015	25.831057853652396
25	25.28684875560913	24.15220907648134	24.72300231989899	25.837939848010542
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	182.0
1	182.0
2	595.5
3	1009.0
4	1009.0
5	1009.0
6	2278.5
7	3548.0
8	3548.0
9	3548.0
10	4472.5
11	5397.0
12	5397.0
13	5397.0
14	7324.0
15	9251.0
16	9251.0
17	9251.0
18	15831.0
19	22411.0
20	22411.0
21	22411.0
22	36167.0
23	49923.0
24	49923.0
25	49923.0
26	75539.0
27	101155.0
28	101155.0
29	101155.0
30	138315.5
31	175476.0
32	175476.0
33	175476.0
34	229809.5
35	284143.0
36	284143.0
37	284143.0
38	360334.0
39	436525.0
40	436525.0
41	436525.0
42	519384.5
43	602244.0
44	602244.0
45	602244.0
46	686946.0
47	771648.0
48	771648.0
49	771648.0
50	820434.5
51	869221.0
52	869221.0
53	869221.0
54	845783.0
55	822345.0
56	822345.0
57	822345.0
58	760863.0
59	699381.0
60	699381.0
61	699381.0
62	607121.0
63	514861.0
64	514861.0
65	514861.0
66	415572.5
67	316284.0
68	316284.0
69	316284.0
70	239239.5
71	162195.0
72	162195.0
73	162195.0
74	118260.0
75	74325.0
76	74325.0
77	74325.0
78	54979.5
79	35634.0
80	35634.0
81	35634.0
82	24297.0
83	12960.0
84	12960.0
85	12960.0
86	8988.5
87	5017.0
88	5017.0
89	5017.0
90	3288.5
91	1560.0
92	1560.0
93	1560.0
94	970.0
95	380.0
96	380.0
97	380.0
98	1327.0
99	2274.0
100	2274.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.03108348417194E-4
2	1.8396651541831727E-4
3	7.358660616732691E-4
4	0.010335573320774552
5	0.034401738383225335
6	0.06356879319136581
7	0.10604833402432272
8	0.10835627758138888
9	0.11775529409639746
10	0.1323388215004677
11	0.1394633429157589
12	0.1325395122445604
13	0.12750551941356827
14	0.11646752848846922
15	0.1325562364732348
16	0.12019703148285876
17	0.12421084636471294
18	0.11252061052131261
19	0.09820467077603263
20	0.09308705680166854
21	0.09253515725541359
22	0.10379056315327974
23	0.08330338302714894
24	0.09226756959662331
25	0.09000979872558032
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5979349.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.14847493833382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.94029686252614	26.875508309497565
2	15.492764458633147	12.440217311858836
3	6.369948902926412	7.672312016628243
4	3.280733243965611	5.268657456988477
5	1.9056009413958341	3.8253485819047994
6	1.2186526577488956	2.9356227412899267
7	0.8310581862906596	2.3356003135211396
8	0.5958114080197573	1.913673550628369
9	0.4588339993056813	1.657933678978166
>10	2.5992759821843285	19.306846149576785
>50	0.2000760828495178	5.511572131145566
>100	0.09886958040877011	6.949809242333534
>500	0.005399095512279334	1.4290199450964542
>1k	0.0026367451356184546	1.7423929150816655
>5k	4.185309738326127E-5	0.1354856554704524
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8063	0.13484745580162658	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.672422867439248E-4
2	0.0	0.0	0.0	0.0	1.8396651541831727E-4
3	0.0	0.0	0.0	0.0	1.8396651541831727E-4
4	0.0	0.0	0.0	0.0	1.8396651541831727E-4
5	1.672422867439248E-5	0.0	0.0	0.0	2.0069074409270976E-4
6	1.672422867439248E-5	0.0	0.0	0.0	3.5120880216224206E-4
7	1.672422867439248E-5	0.0	0.0	0.0	3.5120880216224206E-4
8	1.672422867439248E-5	0.0	0.0	0.0	3.5120880216224206E-4
9	1.672422867439248E-5	0.0	0.0	0.0	3.6793303083663454E-4
10	1.672422867439248E-5	0.0	0.0	0.0	3.6793303083663454E-4
11	1.672422867439248E-5	0.0	0.0	0.0	3.6793303083663454E-4
12	1.672422867439248E-5	0.0	0.0	0.0	8.194872050452315E-4
13	1.672422867439248E-5	0.0	0.0	0.0	9.198325770915864E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1865	0.0	14.766604	1
GTATCAA	5165	0.0	12.668069	1
GCGTAAC	325	0.0	12.278817	11
CGCGTAA	320	0.0	11.876634	10
TCTAACG	65	8.044854E-4	11.689608	2
TCGCGTA	335	0.0	11.3447485	9
CGAACGA	135	4.7402864E-9	11.259205	16
CGATATA	60	0.0058729583	11.084858	10
ACGAACG	155	3.1286618E-10	11.032944	15
ACCGTCG	485	0.0	10.969846	8
CGTCGTA	455	0.0	10.858636	10
TAATACG	90	9.532298E-5	10.554709	4
GTGTACG	360	0.0	10.287827	1
TAGGTCG	75	0.0026486425	10.133709	5
GTCTTAG	525	0.0	10.129553	1
AAGACGG	795	0.0	10.038108	5
TCTTATA	465	0.0	10.008441	2
ATACCGT	655	0.0	10.008423	6
CCGTCGT	475	0.0	10.001291	9
CCGACCA	725	0.0	9.828855	9
>>END_MODULE