FastQCFastQC Report
Thu 2 Feb 2017
SRR4062305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5979349
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52800.027.7567251
GGTATCA21400.024.7817061
TCAACGC74300.019.2750994
TATCAAC76850.019.065552
CAACGCA76750.018.6598035
ATCAACG78100.018.421763
AACGCAG80250.017.8185656
TATACCG3700.016.647955
ACCGTCG8600.016.6272098
GCGAAAG14350.016.55679518
GTATAGG10550.016.2693461
AACGTCG2301.8189894E-1216.26059322
TCTAGCG6650.016.2109328
ATACCGT10850.015.8150056
AGCGAAA15200.015.63092117
CGCAATA7050.015.60361736
ACGTCGT2403.6379788E-1215.58306923
ACGGACC15600.015.3711498
TACGAAT7450.015.06153441
CGTATAC2355.0931703E-1114.9780943
Produced by FastQC (version 0.11.5)