##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062305_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5979349
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226476494347462	32.0	32.0	32.0	32.0	32.0
2	31.273309853631222	32.0	32.0	32.0	32.0	32.0
3	31.47014750267964	32.0	32.0	32.0	32.0	32.0
4	31.57563657849709	32.0	32.0	32.0	32.0	32.0
5	31.516535328511516	32.0	32.0	32.0	32.0	32.0
6	35.15796886918626	36.0	36.0	36.0	36.0	36.0
7	35.16731102332378	36.0	36.0	36.0	36.0	36.0
8	35.09684365304651	36.0	36.0	36.0	36.0	36.0
9	35.22462779810979	36.0	36.0	36.0	36.0	36.0
10	35.069797063192	36.0	36.0	36.0	36.0	36.0
11	35.22598680893188	36.0	36.0	36.0	36.0	36.0
12	35.1389291710519	36.0	36.0	36.0	36.0	36.0
13	35.18306491225048	36.0	36.0	36.0	36.0	36.0
14	35.1384737703051	36.0	36.0	36.0	36.0	36.0
15	35.10179419197642	36.0	36.0	36.0	36.0	36.0
16	35.12206395712978	36.0	36.0	36.0	36.0	36.0
17	35.104103640714065	36.0	36.0	36.0	36.0	36.0
18	35.09953759180138	36.0	36.0	36.0	36.0	36.0
19	35.09240454102947	36.0	36.0	36.0	36.0	36.0
20	35.09717362207826	36.0	36.0	36.0	36.0	36.0
21	35.090559524122106	36.0	36.0	36.0	36.0	36.0
22	35.071061080395204	36.0	36.0	36.0	36.0	36.0
23	35.03978710725867	36.0	36.0	36.0	36.0	36.0
24	35.021131062930095	36.0	36.0	36.0	36.0	36.0
25	35.010177863844376	36.0	36.0	36.0	36.0	36.0
26	34.96444763468398	36.0	36.0	36.0	36.0	36.0
27	34.9474544804125	36.0	36.0	36.0	32.0	36.0
28	34.93380215806102	36.0	36.0	36.0	32.0	36.0
29	34.91288282386594	36.0	36.0	36.0	32.0	36.0
30	34.89295741058098	36.0	36.0	36.0	32.0	36.0
31	34.894923678146235	36.0	36.0	36.0	32.0	36.0
32	34.86552265137894	36.0	36.0	36.0	32.0	36.0
33	34.84617790331355	36.0	36.0	36.0	32.0	36.0
34	34.83898681946814	36.0	36.0	36.0	32.0	36.0
35	34.82755848504578	36.0	36.0	36.0	32.0	36.0
36	34.80266681205596	36.0	36.0	36.0	32.0	36.0
37	34.79534076368515	36.0	36.0	36.0	32.0	36.0
38	34.78245140064579	36.0	36.0	36.0	32.0	36.0
39	34.76714973486244	36.0	36.0	36.0	32.0	36.0
40	34.75479788853268	36.0	36.0	36.0	32.0	36.0
41	34.72968562296665	36.0	36.0	36.0	32.0	36.0
42	34.70501036149587	36.0	36.0	36.0	32.0	36.0
43	34.6927672226525	36.0	36.0	36.0	32.0	36.0
44	34.658613504580515	36.0	36.0	36.0	32.0	36.0
45	34.64538664660651	36.0	36.0	36.0	32.0	36.0
46	34.631028060078116	36.0	36.0	36.0	32.0	36.0
47	34.60548514562371	36.0	36.0	36.0	32.0	36.0
48	34.58569570031788	36.0	36.0	36.0	32.0	36.0
49	34.57093104951726	36.0	36.0	36.0	32.0	36.0
50	34.11554000276619	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	6.0
20	27.0
21	91.0
22	287.0
23	866.0
24	2367.0
25	5743.0
26	12714.0
27	25168.0
28	44700.0
29	74363.0
30	115651.0
31	180169.0
32	284993.0
33	502083.0
34	1144434.0
35	3585683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.821591353367545	16.625180822682772	11.922327257981166	26.630900565968513
2	16.714430337320504	18.273329507779696	36.60437681915194	28.40786333574786
3	19.832914473905365	21.03691164215139	27.30339654941446	31.82677733452878
4	13.291616410351095	14.42963346505338	33.32099085050745	38.957759274088076
5	15.550326816623954	35.54834666728658	32.446887594916305	16.454438921173164
6	37.82645089052396	32.95247221894706	14.950617500746318	14.27045938978266
7	32.78470616115567	28.909702377298935	18.340441409257092	19.9651500522883
8	29.14670142184375	33.40015777637331	17.297903166381488	20.155237635401445
9	26.683138916962363	14.605636834377789	17.41276516891722	41.29845907974263
10	16.143256493813286	26.72515133346545	30.56199299421592	26.56959917850534
11	38.6186857465587	20.81569414998188	20.613046671134267	19.952573432325156
12	25.047843837180267	22.545949400177175	27.728286139511177	24.67792062313138
13	30.000590365272206	18.79405266359264	24.48463871234143	26.720718258793724
14	23.547346040513776	18.24215311733769	24.231049232951616	33.97945160919692
15	25.568352006213384	26.63781625725476	21.236676434173688	26.557155302358165
16	26.38740135146074	25.716504317890966	22.416256195532995	25.4798381351153
17	24.92898474399136	25.792724258109033	24.06430867306792	25.213982324831687
18	25.516812892392803	25.04883482123445	24.573932908983267	24.86041937738948
19	25.945817847394427	24.699461429663998	24.511430926677804	24.84328979626377
20	26.178213701976826	24.21170476209112	24.358142647157447	25.251938888774607
21	26.35929976969057	23.93239596230157	24.382328036824088	25.325976231183773
22	26.218439499015695	23.73495843778311	24.53166724337382	25.514934819827378
23	25.306643016369513	23.910938769735225	24.849201760660485	25.933216453234774
24	25.283261150825986	24.292223421919132	24.664859762567048	25.759655664687838
25	25.532274999188875	23.951028659713607	24.753375543162498	25.76332079793502
26	25.321972177108403	24.46766112740895	24.819390027730574	25.390976667752074
27	25.584317432379933	24.419788380866276	24.458923219954354	25.536970966799437
28	25.6252509725736	24.226761967534667	24.62830136075845	25.519685699133284
29	25.316838238774253	24.548940655891403	24.75215798202904	25.382063123305304
30	25.4723402689626	24.56324935987169	24.55358262797967	25.410827743186044
31	25.612273517041174	24.267921749538786	24.434596416009665	25.68520831741038
32	25.63402499189286	24.402475942413986	24.215932335880844	25.74756672981231
33	25.188223429715368	24.400676998927974	24.58451030466624	25.826589266690412
34	25.659528771478037	24.27836536909712	24.679264670638254	25.382841188786593
35	25.758148690448017	24.336134369457476	24.699920643349135	25.205796296745376
36	25.339812092784914	24.474280914897882	24.628629208537273	25.55727778377993
37	25.86816072249864	24.162846510850024	24.47531044863486	25.493682318016475
38	25.406984172856305	24.212021109993934	24.717377212364962	25.663617504784803
39	25.746323322817073	24.37228564088979	24.35669856331297	25.52469247298017
40	25.832340749199496	24.35099046015431	24.385793620769643	25.43087516987655
41	25.27566441516965	24.5477675325846	24.72237046561284	25.454197586632908
42	25.808516894200633	24.468668350104718	24.3689699707344	25.353844784960245
43	25.53712596112018	24.07626844950177	24.630248979318093	25.756356610059957
44	25.31192027150611	24.44292544754287	24.517251114383253	25.727903166567767
45	25.43676323903551	24.557399871421186	24.41689640708508	25.588940482458224
46	25.488529848817947	24.31695465040143	24.587475111699444	25.607040389081178
47	25.50664109837937	24.51250835802693	24.631569476067526	25.34928106752617
48	25.710155146680545	24.741767846618128	24.129386350758207	25.41869065594312
49	25.50538850829546	24.615072503229158	24.22585089126096	25.65368809721442
50	25.45996207212184	24.75689648338427	24.371346004837065	25.411795439656824
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	33.0
2	46.0
3	141.0
4	236.0
5	306.5
6	377.0
7	337.5
8	298.0
9	380.5
10	463.0
11	893.5
12	1324.0
13	2411.0
14	3498.0
15	4815.5
16	6133.0
17	5958.0
18	5783.0
19	5612.0
20	5441.0
21	6599.0
22	7757.0
23	9969.5
24	12182.0
25	16292.0
26	20402.0
27	25576.0
28	30750.0
29	37690.5
30	44631.0
31	54501.5
32	64372.0
33	77004.0
34	89636.0
35	107936.0
36	126236.0
37	146568.5
38	166901.0
39	192995.5
40	219090.0
41	245630.5
42	272171.0
43	296207.0
44	320243.0
45	352711.0
46	385179.0
47	413333.0
48	441487.0
49	462937.0
50	484387.0
51	494202.5
52	504018.0
53	507884.5
54	511751.0
55	501942.5
56	492134.0
57	471203.0
58	450272.0
59	418373.5
60	386475.0
61	340788.5
62	295102.0
63	255412.0
64	215722.0
65	181307.5
66	146893.0
67	123215.0
68	99537.0
69	83516.0
70	67495.0
71	52848.5
72	38202.0
73	32502.5
74	26803.0
75	20600.0
76	14397.0
77	11865.0
78	9333.0
79	7641.0
80	5949.0
81	4532.0
82	3115.0
83	2398.5
84	1682.0
85	1259.5
86	837.0
87	572.5
88	308.0
89	208.5
90	109.0
91	85.0
92	61.0
93	48.0
94	35.0
95	37.5
96	40.0
97	31.5
98	23.0
99	18.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030237405443301606
2	0.00431485099799326
3	2.6758765879027966E-4
4	1.672422867439248E-5
5	3.344845734878496E-5
6	6.689691469756992E-5
7	0.0
8	0.0
9	0.0
10	6.689691469756992E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.856933756500917E-4
17	0.0
18	1.5051805806953232E-4
19	0.0
20	3.6793303083663454E-4
21	3.344845734878496E-5
22	0.0
23	3.344845734878496E-5
24	0.0010201779491379411
25	2.508634301158872E-4
26	4.682784028829894E-4
27	3.6793303083663454E-4
28	0.0018396651541831728
29	6.020722322781293E-4
30	0.0013212140652770058
31	6.020722322781293E-4
32	8.362114337196239E-4
33	3.1776034481345713E-4
34	7.024176043244842E-4
35	2.341392014414947E-4
36	2.508634301158872E-4
37	0.0016556986387648554
38	1.672422867439248E-5
39	6.187964609525218E-4
40	1.1706960072074736E-4
41	0.0011372475498586886
42	0.005268132032433631
43	0.00431485099799326
44	0.0042981267693188675
45	0.0022912193283917697
46	0.0022410466423685924
47	0.0011205233211842962
48	0.0038967452811334478
49	0.003946917967156625
50	0.001772768239485603
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5979349.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.434084330633766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.48070998299767	27.545673437959316
2	16.113560798937478	13.353012740199397
3	6.540654385650228	8.130180761776835
4	3.254148159313345	5.393305970294631
5	1.8546713611926076	3.842330479263291
6	1.1620767447796962	2.8889751505122185
7	0.813412474028969	2.3592100731154027
8	0.5687672957092841	1.8853081675939993
9	0.4467005989919599	1.6657767260259735
>10	2.487879699589163	19.07965805939198
>50	0.1841156614764861	5.234840905401756
>100	0.08728223460583595	6.273625525511567
>500	0.004352745262732132	1.207918000993668
>1k	0.0016678574643383	1.140184001959846
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.36211433719624E-5
2	0.0	0.0	0.0	0.0	8.36211433719624E-5
3	0.0	0.0	0.0	0.0	8.36211433719624E-5
4	0.0	0.0	0.0	0.0	8.36211433719624E-5
5	0.0	1.672422867439248E-5	0.0	0.0	8.36211433719624E-5
6	0.0	1.672422867439248E-5	0.0	0.0	1.5051805806953232E-4
7	0.0	3.344845734878496E-5	0.0	0.0	1.5051805806953232E-4
8	0.0	3.344845734878496E-5	0.0	0.0	1.5051805806953232E-4
9	0.0	3.344845734878496E-5	0.0	0.0	1.672422867439248E-4
10	0.0	3.344845734878496E-5	0.0	0.0	1.8396651541831727E-4
11	0.0	3.344845734878496E-5	0.0	0.0	1.8396651541831727E-4
12	0.0	3.344845734878496E-5	0.0	0.0	5.184510889061669E-4
13	0.0	3.344845734878496E-5	0.0	0.0	6.522449183013067E-4
14	0.0	3.344845734878496E-5	0.0	0.0	6.689691469756992E-4
15	0.0	3.344845734878496E-5	0.0	1.672422867439248E-5	8.529356623940165E-4
16	0.0	3.344845734878496E-5	0.0	5.017268602317744E-5	8.863841197428014E-4
17	0.0	3.344845734878496E-5	0.0	1.0034537204635488E-4	8.863841197428014E-4
18	0.0	3.344845734878496E-5	0.0	1.672422867439248E-4	9.532810344403714E-4
19	0.0	3.344845734878496E-5	0.0	2.508634301158872E-4	9.700052631147638E-4
20	0.0	3.344845734878496E-5	0.0	3.5120880216224206E-4	0.0011037990925099037
21	0.0	3.344845734878496E-5	0.0	4.5155417420859694E-4	0.0012041444645562586
22	0.0	3.344845734878496E-5	0.0	6.020722322781293E-4	0.001220868693230651
23	0.0	3.344845734878496E-5	0.0	7.693145190220541E-4	0.001254317150579436
24	0.0	3.344845734878496E-5	0.0	9.031083484171939E-4	0.001254317150579436
25	0.0	3.344845734878496E-5	0.0	0.0011874202358818661	0.001254317150579436
26	0.0	3.344845734878496E-5	0.0	0.001672422867439248	0.0014048352086489682
27	0.0	3.344845734878496E-5	0.0	0.0020236316696014903	0.0015051805806953233
28	0.0	3.344845734878496E-5	0.0	0.0023915647004381247	0.0015219048093697157
29	0.0	3.344845734878496E-5	0.0	0.0031274307621113936	0.0015219048093697157
30	0.0	3.344845734878496E-5	0.0	0.004231229854621298	0.0015219048093697157
31	0.0	3.344845734878496E-5	0.0	0.00620468883819961	0.0015888017240672857
32	0.0	3.344845734878496E-5	0.0	0.008746771596707267	0.0016891470961136405
33	0.0	3.344845734878496E-5	0.0	0.011456096641958848	0.001739319782136818
34	1.672422867439248E-5	3.344845734878496E-5	0.0	0.014198870144559216	0.0018062166968343877
35	1.672422867439248E-5	3.344845734878496E-5	0.0	0.01705871324788033	0.0018062166968343877
36	1.672422867439248E-5	3.344845734878496E-5	0.0	0.02117287350178088	0.0018062166968343877
37	1.672422867439248E-5	5.017268602317744E-5	0.0	0.02710997468119021	0.0018062166968343877
38	1.672422867439248E-5	5.017268602317744E-5	0.0	0.03585674627789748	0.0019232862975551352
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5280	0.0	27.756725	1
GGTATCA	2140	0.0	24.781706	1
TCAACGC	7430	0.0	19.275099	4
TATCAAC	7685	0.0	19.06555	2
CAACGCA	7675	0.0	18.659803	5
ATCAACG	7810	0.0	18.42176	3
AACGCAG	8025	0.0	17.818565	6
TATACCG	370	0.0	16.64795	5
ACCGTCG	860	0.0	16.627209	8
GCGAAAG	1435	0.0	16.556795	18
GTATAGG	1055	0.0	16.269346	1
AACGTCG	230	1.8189894E-12	16.260593	22
TCTAGCG	665	0.0	16.21093	28
ATACCGT	1085	0.0	15.815005	6
AGCGAAA	1520	0.0	15.630921	17
CGCAATA	705	0.0	15.603617	36
ACGTCGT	240	3.6379788E-12	15.583069	23
ACGGACC	1560	0.0	15.371149	8
TACGAAT	745	0.0	15.061534	41
CGTATAC	235	5.0931703E-11	14.978094	3
>>END_MODULE