FastQCFastQC Report
Thu 2 Feb 2017
SRR4062304_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062304_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243166
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14200.11422448812145763No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCGT250.00602454319.0037467
AATACCG250.006025945319.0029815
GTACCGT400.0052714114.25281056
ACCCGTC400.00527281914.2522368
GATAAAC400.005274228314.2516623
AGCGCTA400.005282691714.24821914
GGTATCA2850.013.6633061
TTATAGT1051.366061E-913.5735584
CCCTTAT852.7056194E-713.4084681
GAACCGT500.001497024613.3026236
TACCGTG655.4406897E-513.156447
CGTGCAA655.4468626E-513.1548510
GTATCAA8850.012.8781881
GTCTAGT751.4817579E-512.6635521
AGAACCG701.08896376E-412.2162025
GTTATAC550.003065169812.0923193
GACCGTG802.859495E-511.8773427
GTACTAA802.8724904E-511.8720811
GAACGCA658.010876E-411.6941429
TCTAGTA658.0271345E-411.6913172
Produced by FastQC (version 0.11.5)