##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062304_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1243166
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203712134984386	32.0	32.0	32.0	32.0	32.0
2	30.891063623039884	32.0	32.0	32.0	32.0	32.0
3	30.90116927264742	32.0	32.0	32.0	32.0	32.0
4	30.921458598449444	32.0	32.0	32.0	32.0	32.0
5	30.882761433308183	32.0	32.0	32.0	32.0	32.0
6	34.37988973314907	36.0	36.0	36.0	32.0	36.0
7	34.3714403386193	36.0	36.0	36.0	32.0	36.0
8	34.3602688619219	36.0	36.0	36.0	32.0	36.0
9	34.45179565721714	36.0	36.0	36.0	32.0	36.0
10	34.27344216299352	36.0	36.0	36.0	32.0	36.0
11	34.39135803263603	36.0	36.0	36.0	32.0	36.0
12	34.31161405636898	36.0	36.0	36.0	32.0	36.0
13	34.34618868276642	36.0	36.0	36.0	32.0	36.0
14	34.304725997976135	36.0	36.0	36.0	32.0	36.0
15	34.25932659033468	36.0	36.0	36.0	32.0	36.0
16	34.25564566598507	36.0	36.0	36.0	32.0	36.0
17	34.21306406384988	36.0	36.0	36.0	32.0	36.0
18	34.21286618199018	36.0	36.0	36.0	32.0	36.0
19	34.20197141813724	36.0	36.0	36.0	32.0	36.0
20	34.189478315848405	36.0	36.0	36.0	32.0	36.0
21	34.1719102678162	36.0	36.0	36.0	32.0	36.0
22	34.14110022313995	36.0	36.0	36.0	32.0	36.0
23	34.11424781565776	36.0	36.0	36.0	32.0	36.0
24	34.08776382236966	36.0	36.0	36.0	32.0	36.0
25	33.69133727917269	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	21.0
5	98.0
6	240.0
7	55.0
8	205.0
9	150.0
10	105.0
11	26.0
12	50.0
13	63.0
14	181.0
15	351.0
16	526.0
17	679.0
18	904.0
19	1183.0
20	1534.0
21	1975.0
22	2809.0
23	3974.0
24	5398.0
25	7847.0
26	11129.0
27	15219.0
28	21528.0
29	30363.0
30	41906.0
31	60540.0
32	89931.0
33	132702.0
34	293017.0
35	518455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.54277879356571	17.24279673185317	11.838223996923945	26.37620047765718
2	16.566478517263654	19.189388985014812	37.56528719932495	26.678845298396585
3	19.475979301085864	22.00228933272306	28.436553066105084	30.085178300085992
4	13.418067960399608	15.064222722438453	34.74631903962774	36.771390277534195
5	15.0591789062544	35.84393409170095	33.91137607754372	15.185510924500928
6	35.4005927075402	34.26097482844159	16.148141470693815	14.190290993324394
7	30.582292872798366	30.427854334988304	19.813224383574436	19.176628408638898
8	27.70763210023674	33.965986504114795	18.861143768218643	19.465237627429826
9	26.56653915844574	15.279162472317296	18.519186631769678	39.635111737467284
10	15.791483058663008	27.76410757851684	32.01218800063469	24.432221362185462
11	36.5187938735154	21.798718480541606	22.145316735065386	19.53717091087761
12	23.985076983050522	23.619008804966608	29.152960923732174	23.2429532882507
13	29.392079908056125	20.08936661332268	24.807289145612973	25.71126433300822
14	22.922792089557404	19.42297610390489	25.578316425756853	32.07591538078085
15	24.66363667848217	27.40882852538159	22.261472999519164	25.66606179661708
16	25.06525270372897	26.654269793857026	23.489362661682147	24.79111484073186
17	23.740469511699136	26.501180262436247	25.395736236513155	24.362613989351463
18	24.008217077735296	26.355608453507962	25.585116491813526	24.05105797694322
19	25.116125525876125	25.68457497131585	25.306548038406568	23.892751464401456
20	24.98613160841942	25.542397021657237	25.32726586169611	24.144205508227238
21	24.861278396465217	25.169396548947876	25.366489111602053	24.602835942984857
22	25.143401902148533	25.143482419780284	25.206849795968324	24.506265882102863
23	24.405822349274946	25.165455220221883	25.473220848203038	24.955501582300137
24	24.340526805640987	25.39161001310734	25.26319348948353	25.00466969176814
25	24.51959770836755	25.373485207119646	25.38870156929805	24.71821551521475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	84.0
1	84.0
2	224.5
3	365.0
4	365.0
5	365.0
6	657.5
7	950.0
8	950.0
9	950.0
10	1349.0
11	1748.0
12	1748.0
13	1748.0
14	2570.0
15	3392.0
16	3392.0
17	3392.0
18	5829.0
19	8266.0
20	8266.0
21	8266.0
22	12422.5
23	16579.0
24	16579.0
25	16579.0
26	23951.0
27	31323.0
28	31323.0
29	31323.0
30	41815.5
31	52308.0
32	52308.0
33	52308.0
34	64307.5
35	76307.0
36	76307.0
37	76307.0
38	91948.5
39	107590.0
40	107590.0
41	107590.0
42	122219.0
43	136848.0
44	136848.0
45	136848.0
46	149848.5
47	162849.0
48	162849.0
49	162849.0
50	167728.5
51	172608.0
52	172608.0
53	172608.0
54	164705.0
55	156802.0
56	156802.0
57	156802.0
58	142093.5
59	127385.0
60	127385.0
61	127385.0
62	108388.0
63	89391.0
64	89391.0
65	89391.0
66	71657.5
67	53924.0
68	53924.0
69	53924.0
70	39734.5
71	25545.0
72	25545.0
73	25545.0
74	18283.5
75	11022.0
76	11022.0
77	11022.0
78	7923.0
79	4824.0
80	4824.0
81	4824.0
82	3305.0
83	1786.0
84	1786.0
85	1786.0
86	1190.0
87	594.0
88	594.0
89	594.0
90	374.5
91	155.0
92	155.0
93	155.0
94	110.5
95	66.0
96	66.0
97	66.0
98	260.5
99	455.0
100	455.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012065967055083552
2	4.0219890183611847E-4
3	7.239580233050131E-4
4	0.011905087494349105
5	0.03289987017019449
6	0.058881919228807734
7	0.10038884589829515
8	0.10505435315959412
9	0.11390272899998873
10	0.1300711248538007
11	0.13441487299363078
12	0.12822100990535457
13	0.12379682198515726
14	0.11382228921962151
15	0.12685353363911175
16	0.11703988043431048
17	0.12009659208826497
18	0.10955898086015867
19	0.09580377841736341
20	0.09113827115606443
21	0.08928815620761829
22	0.09668861600140287
23	0.07939406322244977
24	0.08960991532908719
25	0.08703584235733602
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1243166.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.52059922899224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.87587880095619	31.478165149298686
2	16.574760725046573	16.084346449128514
3	7.381681185229508	10.7449078327414
4	3.8341241012593277	7.441359956456957
5	2.2083787589317825	5.3575930353974135
6	1.3838266878430103	4.028646007392867
7	0.8791190986818834	2.9858769823187576
8	0.611611721348248	2.374061377223393
9	0.4325720914283048	1.8889791377255785
>10	1.7447113626238846	13.840531811167647
>50	0.051246799903905085	1.6771521580575128
>100	0.021424348806477245	1.8326987386317426
>500	4.982384557364158E-4	0.15125384321824142
>1k	1.6607948524547192E-4	0.1144275212412908
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1420	0.11422448812145763	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	8.043978036722369E-5	0.0
12	0.0	0.0	0.0	8.043978036722369E-5	0.0
13	0.0	0.0	0.0	8.043978036722369E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCGT	25	0.006024543	19.003746	7
AATACCG	25	0.0060259453	19.002981	5
GTACCGT	40	0.00527141	14.2528105	6
ACCCGTC	40	0.005272819	14.252236	8
GATAAAC	40	0.0052742283	14.251662	3
AGCGCTA	40	0.0052826917	14.248219	14
GGTATCA	285	0.0	13.663306	1
TTATAGT	105	1.366061E-9	13.573558	4
CCCTTAT	85	2.7056194E-7	13.408468	1
GAACCGT	50	0.0014970246	13.302623	6
TACCGTG	65	5.4406897E-5	13.15644	7
CGTGCAA	65	5.4468626E-5	13.15485	10
GTATCAA	885	0.0	12.878188	1
GTCTAGT	75	1.4817579E-5	12.663552	1
AGAACCG	70	1.08896376E-4	12.216202	5
GTTATAC	55	0.0030651698	12.092319	3
GACCGTG	80	2.859495E-5	11.877342	7
GTACTAA	80	2.8724904E-5	11.872081	1
GAACGCA	65	8.010876E-4	11.694142	9
TCTAGTA	65	8.0271345E-4	11.691317	2
>>END_MODULE