FastQCFastQC Report
Thu 2 Feb 2017
SRR4062304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243166
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8000.028.3355921
GTATAGG1306.730261E-1122.0082281
GGTATCA4000.022.0082261
GACTAAT600.00739624718.340191
TATCAAC12750.017.9467542
CAACGCA12500.017.4235065
TCAACGC12600.017.2852254
ATCAACG13150.016.8968583
CCTATAC1053.7754005E-516.7614293
AACGCAG12950.016.6481766
GTAGGAC2251.8189894E-1216.6217523
CCCCTAC2252.5465852E-1115.6502951
GTATTAT1552.6561065E-715.6187431
GCCGTAC850.00297258615.535221
TAGGACT3350.015.1040494
CCCTATA900.004356425714.6680222
TAGTACT1351.9565829E-514.6662524
TATAATA2305.9299055E-1014.3491512
TTAATAC1851.265962E-714.2698663
ATAGAAC1402.7574999E-514.1424563
Produced by FastQC (version 0.11.5)