##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062304_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1243166
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10274251387184	32.0	32.0	32.0	32.0	32.0
2	31.07507605581234	32.0	32.0	32.0	32.0	32.0
3	31.34763659881303	32.0	32.0	32.0	32.0	32.0
4	31.487797285318294	32.0	32.0	32.0	32.0	32.0
5	31.415008132461796	32.0	32.0	32.0	32.0	32.0
6	35.009726778242005	36.0	36.0	36.0	36.0	36.0
7	35.02534898798712	36.0	36.0	36.0	36.0	36.0
8	34.957087790367495	36.0	36.0	36.0	36.0	36.0
9	35.10397082931805	36.0	36.0	36.0	36.0	36.0
10	34.91565245510254	36.0	36.0	36.0	32.0	36.0
11	35.098415658085884	36.0	36.0	36.0	36.0	36.0
12	35.00896420912412	36.0	36.0	36.0	36.0	36.0
13	35.04955412229742	36.0	36.0	36.0	36.0	36.0
14	35.00897064430655	36.0	36.0	36.0	36.0	36.0
15	34.950492532775186	36.0	36.0	36.0	36.0	36.0
16	34.98370933568003	36.0	36.0	36.0	36.0	36.0
17	34.965361021778264	36.0	36.0	36.0	36.0	36.0
18	34.96230994090894	36.0	36.0	36.0	36.0	36.0
19	34.9533642329343	36.0	36.0	36.0	32.0	36.0
20	34.95764443364764	36.0	36.0	36.0	32.0	36.0
21	34.951100657514765	36.0	36.0	36.0	32.0	36.0
22	34.92670327212939	36.0	36.0	36.0	32.0	36.0
23	34.8903002495242	36.0	36.0	36.0	32.0	36.0
24	34.880925797520206	36.0	36.0	36.0	32.0	36.0
25	34.87038496870088	36.0	36.0	36.0	32.0	36.0
26	34.81681931455655	36.0	36.0	36.0	32.0	36.0
27	34.8019146276523	36.0	36.0	36.0	32.0	36.0
28	34.77689544276468	36.0	36.0	36.0	32.0	36.0
29	34.76209934956393	36.0	36.0	36.0	32.0	36.0
30	34.74073695709181	36.0	36.0	36.0	32.0	36.0
31	34.742769670341694	36.0	36.0	36.0	32.0	36.0
32	34.69945526180735	36.0	36.0	36.0	32.0	36.0
33	34.67632158537155	36.0	36.0	36.0	32.0	36.0
34	34.67541100705779	36.0	36.0	36.0	32.0	36.0
35	34.66235241311297	36.0	36.0	36.0	32.0	36.0
36	34.63177483940198	36.0	36.0	36.0	32.0	36.0
37	34.6274198296929	36.0	36.0	36.0	32.0	36.0
38	34.6216378182801	36.0	36.0	36.0	32.0	36.0
39	34.58717661197298	36.0	36.0	36.0	32.0	36.0
40	34.581677748587076	36.0	36.0	36.0	32.0	36.0
41	34.55404185764411	36.0	36.0	36.0	32.0	36.0
42	34.54157771367621	36.0	36.0	36.0	32.0	36.0
43	34.52021210361287	36.0	36.0	36.0	32.0	36.0
44	34.4912384991224	36.0	36.0	36.0	32.0	36.0
45	34.46988817261733	36.0	36.0	36.0	32.0	36.0
46	34.46187234850374	36.0	36.0	36.0	32.0	36.0
47	34.42460942464643	36.0	36.0	36.0	32.0	36.0
48	34.407773378615566	36.0	36.0	36.0	32.0	36.0
49	34.386395702585176	36.0	36.0	36.0	32.0	36.0
50	33.91821607090284	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	22.0
22	63.0
23	200.0
24	569.0
25	1390.0
26	3061.0
27	6338.0
28	11125.0
29	18465.0
30	28657.0
31	44191.0
32	69644.0
33	121236.0
34	261434.0
35	676763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.82711947867829	17.942198559590487	12.36006858479886	26.87061337693237
2	16.09605295123666	19.75792892963271	36.93868423127231	27.207333887858315
3	18.86484808118639	22.586480947002972	28.709727759169347	29.838943212641293
4	13.246179512631459	15.624059860066957	34.77821948154953	36.35154114575205
5	15.03178175722309	36.225250690575514	33.387817877902066	15.355149674299328
6	35.54460319733067	34.570876522931044	15.943296207573912	13.941224072164369
7	30.703703286608548	30.475254310365628	19.76888042304889	19.05216197997693
8	27.846482287964765	34.11426953439846	18.54217377244873	19.497074405188044
9	26.69112572255033	15.092192032278875	18.37003264246287	39.84664960270792
10	15.717581457605686	27.741597635706366	32.05720573827063	24.483615168417312
11	36.97583428118208	21.65953702080012	21.909946057083285	19.454682640934518
12	24.124372770812585	23.50739965539598	29.0599968145847	23.308230759206737
13	29.571996016622077	19.90570848945354	24.740541488425517	25.781754005498865
14	23.088549719023845	19.093990665767883	25.41993587340709	32.39752374180117
15	24.82476193847	27.289034610019904	22.159309376221678	25.726894075288413
16	25.314118858393126	26.461678303677726	23.33062518099038	24.89357765693877
17	24.00483925718689	26.40162295300869	25.23653317416982	24.357004615634597
18	24.219472073296785	26.26288545768261	25.3456299043168	24.172012564703802
19	25.167354963054013	25.580332795459338	25.31576635783154	23.936545883655118
20	25.387438202138735	25.326303969059644	25.152956242368273	24.133301586433348
21	25.20693133499468	25.066081279571673	25.29453025581459	24.432457129619053
22	25.197519880691715	25.02296555729484	25.18706270924398	24.59245185276946
23	24.67393735028146	25.03213569225671	25.33418706753563	24.959739889926205
24	24.628486483616722	25.208058830816356	25.180467808597136	24.982986876969782
25	24.83206144327011	25.063326369912875	25.320412528365143	24.78419965845187
26	24.643569612921908	25.31492326007915	25.305029119341032	24.7364780076579
27	24.722904397894723	25.653916662577636	25.1547061809272	24.46847275860044
28	25.055444144398514	25.12180819101262	25.137252914588267	24.685494750000604
29	24.639949805334794	25.41096882139065	25.30173107242833	24.64735030084623
30	25.00372037276124	25.530766477229307	24.97733589134098	24.48817725866847
31	24.88297994711876	25.097995998915668	25.18310149192021	24.83592256204536
32	24.653986058087547	25.587978358342223	25.15770320425883	24.6003323793114
33	24.623621662145943	25.52117017545553	25.036600158949263	24.818608003449263
34	24.676107140041733	25.429189210060187	25.18987932346492	24.704824326433165
35	24.698109018600924	25.36592114958284	25.443545662519185	24.492424169297053
36	24.993182712162444	25.356851836806598	25.15848685972797	24.49147859130299
37	25.01960752768181	25.058782362475817	25.191108036472016	24.730502073370364
38	24.83908021937537	25.38808172038167	25.0267462269721	24.74609183327086
39	24.847867164002615	25.389713447295875	25.025399044529372	24.737020344172137
40	24.822770911343227	25.519219089984034	25.012850265250393	24.645159733422354
41	24.89255126690161	25.097031655848728	25.257591394770223	24.75282568247944
42	24.73051503002143	25.39626357078043	25.142465039352853	24.73075635984529
43	24.755856232704808	25.058401441534205	25.37904627067379	24.806696055087198
44	24.66181864984877	25.340111976317655	25.206496557050002	24.791572816783578
45	24.41667967134889	25.293811969992163	25.468932752654162	24.820575606004784
46	24.758111743204285	25.23014376544481	25.066123095140032	24.945621396210875
47	24.51343244202013	25.442284782089324	25.411395434329165	24.63288734156138
48	24.594788744744058	25.436463341744986	25.237286594906195	24.731461318604765
49	24.623810052432503	25.199859708057616	25.30346981229517	24.872860427214707
50	24.304985761861094	25.418858696526534	25.409849252698812	24.86630628891356
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	22.5
2	27.0
3	60.5
4	94.0
5	102.0
6	110.0
7	90.5
8	71.0
9	107.5
10	144.0
11	236.0
12	328.0
13	607.5
14	887.0
15	1164.0
16	1441.0
17	1495.0
18	1549.0
19	1735.0
20	1921.0
21	2461.0
22	3001.0
23	3911.0
24	4821.0
25	6155.0
26	7489.0
27	9183.0
28	10877.0
29	13050.5
30	15224.0
31	18211.5
32	21199.0
33	24652.5
34	28106.0
35	32365.0
36	36624.0
37	40707.0
38	44790.0
39	50420.0
40	56050.0
41	62098.5
42	68147.0
43	72322.0
44	76497.0
45	81590.0
46	86683.0
47	88227.0
48	89771.0
49	92089.5
50	94408.0
51	96349.5
52	98291.0
53	97963.0
54	97635.0
55	94722.0
56	91809.0
57	86324.5
58	80840.0
59	75168.5
60	69497.0
61	60514.0
62	51531.0
63	43622.5
64	35714.0
65	30387.5
66	25061.0
67	21009.0
68	16957.0
69	13795.5
70	10634.0
71	8340.0
72	6046.0
73	4866.5
74	3687.0
75	3014.0
76	2341.0
77	1914.5
78	1488.0
79	1113.0
80	738.0
81	558.5
82	379.0
83	268.5
84	158.0
85	97.0
86	36.0
87	25.5
88	15.0
89	10.5
90	6.0
91	8.0
92	10.0
93	8.0
94	6.0
95	6.5
96	7.0
97	4.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0261429286193477
2	0.004826386822033421
3	1.6087956073444738E-4
4	0.0
5	0.0
6	3.2175912146889475E-4
7	0.0
8	0.0
9	0.0
10	3.2175912146889475E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.826386822033421E-4
17	0.0
18	8.043978036722369E-5
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	6.435182429377895E-4
25	2.4131934110167106E-4
26	4.826386822033421E-4
27	2.4131934110167106E-4
28	0.001850114948446145
29	4.826386822033421E-4
30	8.848375840394605E-4
31	2.4131934110167106E-4
32	6.435182429377895E-4
33	1.6087956073444738E-4
34	6.435182429377895E-4
35	1.6087956073444738E-4
36	2.4131934110167106E-4
37	0.0016892353877116975
38	0.0
39	7.239580233050131E-4
40	8.043978036722369E-5
41	0.0012065967055083552
42	0.0043437481398300795
43	0.00370022989689229
44	0.00370022989689229
45	0.0019305547288133685
46	0.0024131934110167104
47	0.00152835582697725
48	0.00394154923799396
49	0.003539350336157842
50	0.002091434289547816
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1243166.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.284095869371214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.51122336755229	32.94172636333791
2	16.57202817289933	16.666189067919813
3	7.28802218998618	10.994148194981095
4	3.707630825481573	7.457394555070053
5	2.09073763022336	5.256542571792671
6	1.322229809086346	3.989227828886292
7	0.868140893589366	3.0557575944958484
8	0.5705824257422104	2.2952977117919753
9	0.4038150836419216	1.8274928741413683
>10	1.6096127289672482	12.942442169174312
>50	0.04184747646783452	1.3841269735204789
>100	0.01380827587699016	1.1056076416058296
>500	3.211204853770606E-4	0.08404645328242107
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	1.6087956073444738E-4	0.0
21	0.0	0.0	0.0	1.6087956073444738E-4	0.0
22	0.0	0.0	0.0	3.2175912146889475E-4	0.0
23	0.0	0.0	0.0	3.2175912146889475E-4	0.0
24	0.0	0.0	0.0	8.848375840394605E-4	0.0
25	0.0	0.0	0.0	0.0012065967055083552	0.0
26	0.0	0.0	0.0	0.0016087956073444736	0.0
27	0.0	0.0	0.0	0.002252313850282263	0.0
28	0.0	0.0	0.0	0.0037002298968922894	0.0
29	0.0	0.0	0.0	0.005711224406072882	0.0
30	0.0	0.0	0.0	0.008204857597456816	0.0
31	0.0	0.0	0.0	0.016248835634179185	0.0
32	0.0	0.0	0.0	0.02485589213347212	0.0
33	0.0	0.0	0.0	0.03402602709533562	0.0
34	0.0	0.0	0.0	0.042793963155363	0.0
35	0.0	0.0	0.0	0.054779490430079326	0.0
36	0.0	0.0	0.0	0.07038480782132073	0.0
37	0.0	0.0	0.0	0.09403410324928449	0.0
38	0.0	0.0	0.0	0.12862320880719066	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	800	0.0	28.335592	1
GTATAGG	130	6.730261E-11	22.008228	1
GGTATCA	400	0.0	22.008226	1
GACTAAT	60	0.007396247	18.34019	1
TATCAAC	1275	0.0	17.946754	2
CAACGCA	1250	0.0	17.423506	5
TCAACGC	1260	0.0	17.285225	4
ATCAACG	1315	0.0	16.896858	3
CCTATAC	105	3.7754005E-5	16.761429	3
AACGCAG	1295	0.0	16.648176	6
GTAGGAC	225	1.8189894E-12	16.621752	3
CCCCTAC	225	2.5465852E-11	15.650295	1
GTATTAT	155	2.6561065E-7	15.618743	1
GCCGTAC	85	0.002972586	15.53522	1
TAGGACT	335	0.0	15.104049	4
CCCTATA	90	0.0043564257	14.668022	2
TAGTACT	135	1.9565829E-5	14.666252	4
TATAATA	230	5.9299055E-10	14.349151	2
TTAATAC	185	1.265962E-7	14.269866	3
ATAGAAC	140	2.7574999E-5	14.142456	3
>>END_MODULE