##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062303_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	765241
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.093152353310916	32.0	32.0	32.0	32.0	32.0
2	30.779404135429232	32.0	32.0	32.0	32.0	32.0
3	30.82371697282294	32.0	32.0	32.0	32.0	32.0
4	30.784637780777558	32.0	32.0	32.0	32.0	32.0
5	30.803646171598228	32.0	32.0	32.0	32.0	32.0
6	34.39918666145698	36.0	36.0	36.0	32.0	36.0
7	34.30035243799012	36.0	36.0	36.0	32.0	36.0
8	34.23676462709133	36.0	36.0	36.0	32.0	36.0
9	34.29180349719892	36.0	36.0	36.0	32.0	36.0
10	34.1054648143526	36.0	36.0	36.0	32.0	36.0
11	34.35324557884379	36.0	36.0	36.0	32.0	36.0
12	34.19454132750336	36.0	36.0	36.0	32.0	36.0
13	34.27867037965817	36.0	36.0	36.0	32.0	36.0
14	34.194197644924934	36.0	36.0	36.0	32.0	36.0
15	34.18036670800441	36.0	36.0	36.0	32.0	36.0
16	34.18084629548077	36.0	36.0	36.0	32.0	36.0
17	34.11108787950462	36.0	36.0	36.0	32.0	36.0
18	34.137780124170035	36.0	36.0	36.0	32.0	36.0
19	34.10188685655891	36.0	36.0	36.0	32.0	36.0
20	34.03893283292453	36.0	36.0	36.0	32.0	36.0
21	34.00766555895463	36.0	36.0	36.0	32.0	36.0
22	34.01008571156015	36.0	36.0	36.0	32.0	36.0
23	34.00416992816642	36.0	36.0	36.0	32.0	36.0
24	34.00300166875533	36.0	36.0	36.0	32.0	36.0
25	33.67343490482084	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	16.0
5	68.0
6	166.0
7	33.0
8	138.0
9	111.0
10	62.0
11	17.0
12	38.0
13	39.0
14	251.0
15	336.0
16	449.0
17	631.0
18	824.0
19	1163.0
20	1604.0
21	2247.0
22	3027.0
23	4093.0
24	5572.0
25	7176.0
26	9505.0
27	11629.0
28	14641.0
29	18599.0
30	23980.0
31	32168.0
32	45162.0
33	64988.0
34	147874.0
35	368634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.17296566901064	17.239328983425832	11.5074350486849	25.08027029887863
2	16.716651181268023	19.905859230539036	37.45310276424166	25.924386823951274
3	19.64951942867224	23.937352577966244	27.732526609472906	28.68060138388861
4	13.153151975743476	15.55933111591769	34.3438911070306	36.94362580130823
5	15.045035623243349	36.55585332374665	32.2234132949866	16.1756977580234
6	34.34026415587812	34.653328102523865	16.659997384595265	14.346410357002744
7	30.639740330761672	30.48444640397092	19.44117735560739	19.434635909660013
8	28.548196971817998	32.55707768531032	18.78072042629518	20.1140049165765
9	27.60147485600789	14.718858844556562	18.36596107699031	39.313705222445236
10	17.187080834424936	26.378265483853657	29.78982342864845	26.644830253072953
11	36.91205955179111	21.502981253958865	21.997937464337504	19.587021729912525
12	25.80384284710399	23.70497256511287	27.51667488906308	22.974509698720055
13	29.72678024652587	19.722462118238205	24.85266022872995	25.698097406505983
14	24.010953856943047	19.879183092436577	24.83210061010314	31.277762440517233
15	25.299930905969305	27.10396557861016	22.366575239211908	25.229528276208622
16	25.580649846324548	26.02394694042574	23.605046887695888	24.790356325553827
17	23.762325712850142	26.206140350877195	24.790646066239585	25.24088787003308
18	24.3141795536018	25.290184996205877	26.31433655179632	24.081298898396003
19	25.60646512424932	24.78655174624021	25.49632360154931	24.110659527961165
20	26.185975876949158	24.00637281768274	25.20886282091362	24.598788484454484
21	26.819585585891975	23.888915776434562	24.763211558912285	24.52828707876118
22	26.269645459468933	24.16558306494023	25.096346801390844	24.468424674199987
23	24.451939953387757	24.56297753352707	25.752086695631476	25.232995817453695
24	24.685287792933494	24.798694062063444	25.814903179561234	24.701114965441825
25	24.625552792772528	24.70141669293563	25.78862486216968	24.88440565212216
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	941.0
1	941.0
2	872.0
3	803.0
4	803.0
5	803.0
6	1439.0
7	2075.0
8	2075.0
9	2075.0
10	2135.5
11	2196.0
12	2196.0
13	2196.0
14	2081.0
15	1966.0
16	1966.0
17	1966.0
18	3213.5
19	4461.0
20	4461.0
21	4461.0
22	7152.0
23	9843.0
24	9843.0
25	9843.0
26	15352.0
27	20861.0
28	20861.0
29	20861.0
30	26827.5
31	32794.0
32	32794.0
33	32794.0
34	39088.5
35	45383.0
36	45383.0
37	45383.0
38	51681.0
39	57979.0
40	57979.0
41	57979.0
42	67056.5
43	76134.0
44	76134.0
45	76134.0
46	86592.0
47	97050.0
48	97050.0
49	97050.0
50	101031.5
51	105013.0
52	105013.0
53	105013.0
54	98033.5
55	91054.0
56	91054.0
57	91054.0
58	84343.5
59	77633.0
60	77633.0
61	77633.0
62	68715.0
63	59797.0
64	59797.0
65	59797.0
66	49108.0
67	38419.0
68	38419.0
69	38419.0
70	29274.0
71	20129.0
72	20129.0
73	20129.0
74	15201.5
75	10274.0
76	10274.0
77	10274.0
78	8189.0
79	6104.0
80	6104.0
81	6104.0
82	4263.5
83	2423.0
84	2423.0
85	2423.0
86	1775.5
87	1128.0
88	1128.0
89	1128.0
90	753.5
91	379.0
92	379.0
93	379.0
94	235.0
95	91.0
96	91.0
97	91.0
98	201.0
99	311.0
100	311.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0018294890106515464
2	5.227111459004418E-4
3	7.840667188506627E-4
4	0.0112382896368595
5	0.038027235864257145
6	0.07069668248303475
7	0.11551916324399764
8	0.11604187438989809
9	0.12584270837553138
10	0.14034794267426864
11	0.14805793207630016
12	0.13956387595541797
13	0.13342201999108777
14	0.12335983043250426
15	0.13917184259599263
16	0.12649609730790692
17	0.12871761967798379
18	0.11774068561407453
19	0.10062189558583505
20	0.09722427313748218
21	0.09670156199158174
22	0.10650239597721502
23	0.08298039441169515
24	0.09578681748625596
25	0.09330393954322887
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	765241.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.67092375293372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9809897114316	39.706280579688205
2	15.151463704050752	16.263861064109015
3	5.1105464079864795	8.22863239796615
4	2.1751000092028367	4.669585069957235
5	1.0907742598185939	2.9271431065193236
6	0.6121506776667605	1.9712815407815645
7	0.3892683366894331	1.4624673852522927
8	0.2616630204817253	1.123495681698961
9	0.16650954627195796	0.8043049045888059
>10	0.8920271209585159	8.503819104966187
>50	0.08585466051571287	3.1925723708523064
>100	0.07704839664309512	7.852921760683489
>500	0.004891961690788136	1.674714102402268
>1k	0.0017121865917758478	1.6189209305341983
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3810	0.4978823664701708	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2267	0.2962465419390754	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1608	0.2101298806519776	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1268	0.16569943325044004	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1241	0.1621711330156121	No Hit
GTACATGGGGTGGTATCAACGCAAA	1127	0.1472738653574495	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1011	0.13211524212633668	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	897	0.11721797446817407	No Hit
GTATCAACGCAGAGTACATGGGGTG	825	0.10780917384196613	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	812	0.1061103626177897	No Hit
GAATAGGACCGCGGTTCTATTTTGT	796	0.10401951803418794	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.613555729502209E-4
13	0.0	0.0	0.0	0.0	2.613555729502209E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCTAA	35	0.0021748873	16.27893	1
CTAAGGT	45	6.7513017E-4	14.779354	4
AACCGTG	40	0.0052675433	14.2533865	7
GGTCTAC	40	0.0052904575	14.244065	1
GTCTAGA	50	0.0015030431	13.29446	1
CTTCTAT	50	0.0015030431	13.29446	1
GTATTAG	80	2.0049702E-6	13.05706	1
AGAACCA	75	1.4736448E-5	12.668848	5
TAGACAT	55	0.0030620408	12.092991	5
TATGCTG	80	2.856857E-5	11.877046	5
TGAACTG	65	8.0036634E-4	11.694322	5
ACCGTCG	130	2.5975169E-9	11.694322	8
TTAAATC	115	7.07314E-8	11.562669	3
TACCGTC	140	6.7484507E-10	11.538456	7
TTTTCGG	75	2.0716508E-4	11.399725	16
CGCCAGT	125	1.8257197E-8	11.399725	18
AACGCCA	100	1.927243E-6	11.39898	15
CGCCCCT	85	5.3051084E-5	11.178395	5
CAGAACC	85	5.3086464E-5	11.177664	4
ATACCGT	145	1.20599E-9	11.140578	6
>>END_MODULE