FastQCFastQC Report
Thu 2 Feb 2017
SRR4062303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765241
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14620.19105092382661149No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9080.11865543011940029No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8660.11316696308744564No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7970.10415019582066304No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7920.10349680688828747No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7910.10336612910181237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAC300.00574546529.3315033
TAGGACG2150.024.5566084
CGTCTTA602.8701176E-422.00150115
CGTATAT703.2149124E-521.99862910
TATACTC500.002580514421.9986295
AGGACGT2450.021.5496755
CGCGGGA1601.8189894E-1220.62506144
TTAGGAC1850.020.2149563
GTAAACG1750.020.11434627
GTATCAA26900.019.5516381
TGTAGGA3800.019.106572
GTATTAA1051.7864077E-618.862131
TTCGGGC1759.094947E-1218.85596735
ATACACT2500.018.4788484
GATATAC1557.166818E-1018.4564931
GATTAAT2150.018.41986338
GGACGTG2750.018.3988536
AGTGCGT600.007412346518.332196
TAGGACC4700.018.2541794
GTAGGAC3500.018.2274343
Produced by FastQC (version 0.11.5)