##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062303_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	765241
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16955834828505	32.0	32.0	32.0	32.0	32.0
2	31.420591682881604	32.0	32.0	32.0	32.0	32.0
3	31.54701982774054	32.0	32.0	32.0	32.0	32.0
4	31.65471400513041	32.0	32.0	32.0	32.0	32.0
5	31.63533187584042	32.0	32.0	32.0	32.0	32.0
6	35.26020560842924	36.0	36.0	36.0	36.0	36.0
7	35.24954752816433	36.0	36.0	36.0	36.0	36.0
8	35.20601614393374	36.0	36.0	36.0	36.0	36.0
9	35.24658244918921	36.0	36.0	36.0	36.0	36.0
10	35.156698347318034	36.0	36.0	36.0	36.0	36.0
11	35.27879452355533	36.0	36.0	36.0	36.0	36.0
12	35.18791727050694	36.0	36.0	36.0	36.0	36.0
13	35.232207892676946	36.0	36.0	36.0	36.0	36.0
14	35.19038969422705	36.0	36.0	36.0	36.0	36.0
15	35.1888084930107	36.0	36.0	36.0	36.0	36.0
16	35.18502145075865	36.0	36.0	36.0	36.0	36.0
17	35.167110753344375	36.0	36.0	36.0	36.0	36.0
18	35.16901864902691	36.0	36.0	36.0	36.0	36.0
19	35.142054594565636	36.0	36.0	36.0	36.0	36.0
20	35.124575133846726	36.0	36.0	36.0	36.0	36.0
21	35.10195480900788	36.0	36.0	36.0	36.0	36.0
22	35.108375008657404	36.0	36.0	36.0	36.0	36.0
23	35.0913882031935	36.0	36.0	36.0	36.0	36.0
24	35.0773599428154	36.0	36.0	36.0	36.0	36.0
25	35.06016405289314	36.0	36.0	36.0	36.0	36.0
26	35.02766710095251	36.0	36.0	36.0	36.0	36.0
27	35.01872874035761	36.0	36.0	36.0	36.0	36.0
28	34.993274014330126	36.0	36.0	36.0	36.0	36.0
29	34.97443419785401	36.0	36.0	36.0	36.0	36.0
30	34.94532310736095	36.0	36.0	36.0	36.0	36.0
31	34.94788177842013	36.0	36.0	36.0	36.0	36.0
32	34.90625698309422	36.0	36.0	36.0	32.0	36.0
33	34.8740004782807	36.0	36.0	36.0	32.0	36.0
34	34.85427597319015	36.0	36.0	36.0	32.0	36.0
35	34.83913041773768	36.0	36.0	36.0	32.0	36.0
36	34.81208534304879	36.0	36.0	36.0	32.0	36.0
37	34.78646596301034	36.0	36.0	36.0	32.0	36.0
38	34.76106481487531	36.0	36.0	36.0	32.0	36.0
39	34.732858014664664	36.0	36.0	36.0	32.0	36.0
40	34.70743857163952	36.0	36.0	36.0	32.0	36.0
41	34.67683122049132	36.0	36.0	36.0	32.0	36.0
42	34.63025504383587	36.0	36.0	36.0	32.0	36.0
43	34.61453711967864	36.0	36.0	36.0	32.0	36.0
44	34.54286166057491	36.0	36.0	36.0	32.0	36.0
45	34.502318877321	36.0	36.0	36.0	32.0	36.0
46	34.43241018189041	36.0	36.0	36.0	32.0	36.0
47	34.37713086465571	36.0	36.0	36.0	32.0	36.0
48	34.317571849913946	36.0	36.0	36.0	32.0	36.0
49	34.277342693347585	36.0	36.0	36.0	32.0	36.0
50	33.88209466037497	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	8.0
21	32.0
22	98.0
23	238.0
24	581.0
25	1241.0
26	2566.0
27	4713.0
28	7731.0
29	11617.0
30	16428.0
31	23151.0
32	33212.0
33	53478.0
34	115726.0
35	494419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58225055032392	17.935461775363265	11.962290393252847	25.51999728105997
2	16.286433809163487	20.27381470460239	37.24896724861439	26.190784237619724
3	19.23226700120224	24.158695311274894	28.08582928231666	28.52320840520621
4	12.862091811599221	15.727071602279544	34.6919467200529	36.71888986606834
5	14.626241929013212	36.60324002503786	32.24069280135278	16.529825244596147
6	34.44984580001045	34.66847002247661	16.448695833986722	14.432988343526215
7	30.59402201397991	30.44191307052288	19.56952123579369	19.394543679703517
8	28.518336053609257	32.387835988923754	18.747165925505822	20.346662031961174
9	27.728911545513114	14.27001428308206	18.43053364887663	39.5705405225282
10	17.154203708374226	26.15136930718558	29.958405260564973	26.736021723875226
11	37.098116802419106	21.30518359575611	21.995554341704114	19.601145260120667
12	25.84270837553137	23.462543172673705	27.676640430923065	23.018108020871857
13	29.83922711929967	19.546260589801122	25.123849872131785	25.490662418767425
14	24.199304532820378	19.44903631666364	25.07510705777657	31.27655209273941
15	25.373836477658674	26.720340389498208	22.645284296058364	25.26053883678475
16	25.63003521793959	25.791423549628544	23.76446451090188	24.814076721529986
17	23.83523621970072	25.703667210721854	25.070925368609366	25.39017120096806
18	24.433150950226008	24.543835324650207	26.937597273531537	24.08541645159225
19	25.729672090230398	24.02524172123553	26.286620816187316	23.95846537234675
20	26.06444008269323	23.477140445194827	25.851434455685684	24.60698501642626
21	26.90420403506869	23.271753604420045	25.444271804568757	24.379770555942507
22	26.301779439418432	23.61047042696353	25.80794808432899	24.27980204928905
23	24.49986344171313	23.76179530370171	26.637098639513564	25.10124261507159
24	24.785001247979384	24.17865314928578	26.39848831146429	24.63785729127055
25	24.744066551844	23.955030983824642	26.46509974674546	24.8358027175859
26	24.131708043772	25.12865278514658	26.74775690700148	23.991882264079933
27	24.923291638135165	24.379015101223676	26.034974831293876	24.662718429347287
28	24.366262339556364	24.341563624673622	26.34046067677092	24.95171335899909
29	24.251500527291757	24.473917026361974	26.424047117798416	24.850535328547853
30	24.190473203516323	24.650595702474153	26.600725793910595	24.558205300098923
31	24.945049488130426	24.302239576391013	25.78714484719707	24.965566088281495
32	24.69293585858388	24.518870772355015	25.625595408445296	25.162597960615813
33	24.326747164288328	24.2420678479954	26.413935497360306	25.01724949035597
34	24.918064492522568	24.358890590615182	26.471441489945587	24.251603426916663
35	25.274129326578166	24.323187074398785	26.430235703523465	23.972447895499588
36	24.209137002770373	24.95556949453766	26.039804505775965	24.795488996916003
37	25.107484024410958	24.74902970348784	25.7165819426839	24.426904329417297
38	24.394537145814194	24.66425609709882	26.064076545820207	24.877130211266778
39	25.268708305291838	24.47248230935595	25.461328872829913	24.797480512522295
40	25.288373205303948	24.8166263961288	25.825066874357226	24.06993352421002
41	24.36823246222724	25.068018410054965	26.347365642404807	24.21638348531299
42	25.43838097652646	25.260650128985553	25.534956782653467	23.766012111834524
43	24.799273411567917	24.56077990643214	25.866548182222104	24.77339849977784
44	24.35887048295113	25.22516635912662	25.50770244063083	24.908260717291416
45	24.56597732692999	25.35829331242445	25.423372210787676	24.652357149857885
46	24.397957210138678	25.037374481853774	25.341336176930273	25.223332131077274
47	24.85981430540945	24.992976013904226	25.573581971551224	24.573627709135103
48	25.285707004771258	25.67057018558377	24.454690038120404	24.58903277152457
49	24.877354570094116	25.688369528001314	24.501642677734594	24.93263322416997
50	24.838673423782502	25.973905475302463	24.479690754791445	24.707730346123586
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	487.0
1	301.0
2	115.0
3	259.0
4	403.0
5	391.5
6	380.0
7	316.0
8	252.0
9	373.0
10	494.0
11	771.5
12	1049.0
13	1886.5
14	2724.0
15	3679.0
16	4634.0
17	4883.0
18	5132.0
19	4802.0
20	4472.0
21	4429.0
22	4386.0
23	4007.5
24	3629.0
25	3686.5
26	3744.0
27	4641.0
28	5538.0
29	6944.0
30	8350.0
31	10134.0
32	11918.0
33	14125.5
34	16333.0
35	18358.0
36	20383.0
37	23417.5
38	26452.0
39	28435.5
40	30419.0
41	32675.5
42	34932.0
43	36211.0
44	37490.0
45	41901.0
46	46312.0
47	49239.0
48	52166.0
49	54884.0
50	57602.0
51	56529.0
52	55456.0
53	55983.0
54	56510.0
55	56798.5
56	57087.0
57	55475.5
58	53864.0
59	49542.0
60	45220.0
61	40331.5
62	35443.0
63	30508.5
64	25574.0
65	21731.0
66	17888.0
67	15367.5
68	12847.0
69	11356.0
70	9865.0
71	7659.0
72	5453.0
73	4753.0
74	4053.0
75	3024.0
76	1995.0
77	1799.0
78	1603.0
79	1347.0
80	1091.0
81	880.0
82	669.0
83	578.5
84	488.0
85	358.0
86	228.0
87	145.5
88	63.0
89	42.5
90	22.0
91	15.0
92	8.0
93	8.0
94	8.0
95	6.5
96	5.0
97	4.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03097063539460118
2	0.006795244896705743
3	1.3067778647511046E-4
4	0.0
5	0.0
6	1.3067778647511046E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.840667188506627E-4
17	0.0
18	2.613555729502209E-4
19	0.0
20	3.9203335942533137E-4
21	0.0
22	0.0
23	0.0
24	5.227111459004418E-4
25	3.9203335942533137E-4
26	3.9203335942533137E-4
27	6.533889323755523E-4
28	0.0024828779430270988
29	0.0013067778647511046
30	0.0013067778647511046
31	9.147445053257732E-4
32	0.0013067778647511046
33	1.3067778647511046E-4
34	6.533889323755523E-4
35	0.0
36	1.3067778647511046E-4
37	0.001437455651226215
38	0.0
39	7.840667188506627E-4
40	0.0
41	9.147445053257732E-4
42	0.0050964336725293075
43	0.0027442335159773195
44	0.003789655807778203
45	0.0020908445836017673
46	0.0016988112241764358
47	7.840667188506627E-4
48	0.004443044740153755
49	0.0035283002348279824
50	0.0030055890889275406
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	765241.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.76618632095675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.76175593853806	41.13411825029451
2	15.471446285875762	17.255671124656438
3	5.133839326600423	8.58883921287163
4	2.1840627117847733	4.871873924881749
5	1.0883032997168294	3.0345262292860373
6	0.6107789372131289	2.0436487208125973
7	0.3675220604008434	1.4346712588162749
8	0.23921354500920272	1.0672021697183816
9	0.1840162354856045	0.9235695306754147
>10	0.8126588691566756	8.089052721068196
>50	0.07768831242924615	3.066897323807234
>100	0.06470022994732963	6.9185689774740835
>500	0.003778115616106756	1.3788415635699447
>1k	2.3613222600667226E-4	0.19251899206754458
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1462	0.19105092382661149	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	908	0.11865543011940029	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	866	0.11316696308744564	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	797	0.10415019582066304	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	792	0.10349680688828747	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	791	0.10336612910181237	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.3067778647511046E-4
7	0.0	0.0	0.0	0.0	1.3067778647511046E-4
8	0.0	0.0	0.0	0.0	1.3067778647511046E-4
9	0.0	0.0	0.0	0.0	1.3067778647511046E-4
10	0.0	0.0	0.0	0.0	1.3067778647511046E-4
11	0.0	0.0	0.0	0.0	1.3067778647511046E-4
12	0.0	0.0	0.0	0.0	5.227111459004418E-4
13	0.0	0.0	0.0	0.0	7.840667188506627E-4
14	0.0	0.0	0.0	0.0	7.840667188506627E-4
15	0.0	0.0	0.0	0.0	9.147445053257732E-4
16	0.0	0.0	0.0	0.0	0.0010454222918008836
17	0.0	0.0	0.0	0.0	0.0010454222918008836
18	0.0	0.0	0.0	1.3067778647511046E-4	0.0010454222918008836
19	0.0	0.0	0.0	1.3067778647511046E-4	0.0010454222918008836
20	0.0	0.0	0.0	1.3067778647511046E-4	0.0010454222918008836
21	0.0	0.0	0.0	3.9203335942533137E-4	0.0013067778647511046
22	0.0	0.0	0.0	6.533889323755523E-4	0.001437455651226215
23	0.0	0.0	0.0	0.0024828779430270988	0.001437455651226215
24	0.0	0.0	0.0	0.004573722526628866	0.001437455651226215
25	0.0	0.0	0.0	0.005227111459004418	0.001437455651226215
26	0.0	0.0	0.0	0.006011178177855081	0.0015681334377013255
27	0.0	0.0	0.0	0.007056600469655965	0.0015681334377013255
28	0.0	0.0	0.0	0.008494056120882179	0.0015681334377013255
29	0.0	0.0	0.0	0.012153034142185272	0.0015681334377013255
30	0.0	0.0	0.0	0.018948279038891017	0.0015681334377013255
31	0.0	0.0	0.0	0.03554435792123004	0.0015681334377013255
32	0.0	0.0	0.0	0.05266314794946951	0.0015681334377013255
33	0.0	0.0	0.0	0.0687365156859081	0.0015681334377013255
34	0.0	0.0	0.0	0.08428717227644625	0.001960166797126657
35	0.0	0.0	0.0	0.10571832925836436	0.001960166797126657
36	0.0	0.0	0.0	0.132507275485762	0.001960166797126657
37	0.0	0.0	0.0	0.1709265447094445	0.001960166797126657
38	0.0	0.0	0.0	0.22215223700768777	0.001960166797126657
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAGAC	30	0.005745465	29.331503	3
TAGGACG	215	0.0	24.556608	4
CGTCTTA	60	2.8701176E-4	22.001501	15
CGTATAT	70	3.2149124E-5	21.998629	10
TATACTC	50	0.0025805144	21.998629	5
AGGACGT	245	0.0	21.549675	5
CGCGGGA	160	1.8189894E-12	20.625061	44
TTAGGAC	185	0.0	20.214956	3
GTAAACG	175	0.0	20.114346	27
GTATCAA	2690	0.0	19.551638	1
TGTAGGA	380	0.0	19.10657	2
GTATTAA	105	1.7864077E-6	18.86213	1
TTCGGGC	175	9.094947E-12	18.855967	35
ATACACT	250	0.0	18.478848	4
GATATAC	155	7.166818E-10	18.456493	1
GATTAAT	215	0.0	18.419863	38
GGACGTG	275	0.0	18.398853	6
AGTGCGT	60	0.0074123465	18.33219	6
TAGGACC	470	0.0	18.254179	4
GTAGGAC	350	0.0	18.227434	3
>>END_MODULE