FastQCFastQC Report
Thu 2 Feb 2017
SRR4062302_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062302_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008394
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20900.20726025739938952No Hit
TATCAACGCAGAGTACTTTTTTTTT12400.12296780821781962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTCA400.00527483814.2510419
CGTTATT801.2880628E-714.248922
GTATACT551.9595101E-413.8178214
GCGTTAT903.6419806E-813.7198211
CGCATCG1351.8189894E-1213.37134913
GCCTATT500.001499764713.2989932
AATAGGC500.001499764713.2989932
TATACTA655.4501103E-513.1535035
CGTCGTA1102.73576E-912.95613610
CCGTCGT1102.737579E-912.9554939
GGTTCTA1251.0186341E-1012.92094513
CCGACCA1650.012.6675939
GGTATCA4650.012.2559191
ACAATAC701.0909376E-412.2133613
GTTCTAG701.0919488E-412.2121491
GTATCAA11450.012.1114181
GTACCGG550.003071181512.0887941
CGGTCCA1501.4551915E-1112.0348110
CGACCAT1603.6379788E-1211.87645810
TCCCGGA1209.997166E-911.8746917
Produced by FastQC (version 0.11.5)