##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062302_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1008394
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20427630469836	32.0	32.0	32.0	32.0	32.0
2	30.761024956515012	32.0	32.0	32.0	32.0	32.0
3	30.77795683036591	32.0	32.0	32.0	32.0	32.0
4	30.780616505056557	32.0	32.0	32.0	32.0	32.0
5	30.712642082360663	32.0	32.0	32.0	32.0	32.0
6	34.33721938051992	36.0	36.0	36.0	32.0	36.0
7	34.274458197886936	36.0	36.0	36.0	32.0	36.0
8	34.22548329323657	36.0	36.0	36.0	32.0	36.0
9	34.34938129342301	36.0	36.0	36.0	32.0	36.0
10	34.05942617667301	36.0	36.0	36.0	32.0	36.0
11	34.35049296207633	36.0	36.0	36.0	32.0	36.0
12	34.166739389564	36.0	36.0	36.0	32.0	36.0
13	34.26415865227282	36.0	36.0	36.0	32.0	36.0
14	34.1678480831897	36.0	36.0	36.0	32.0	36.0
15	34.1204925852395	36.0	36.0	36.0	32.0	36.0
16	34.12842400886955	36.0	36.0	36.0	32.0	36.0
17	34.06115268436742	36.0	36.0	36.0	32.0	36.0
18	34.062971417918	36.0	36.0	36.0	32.0	36.0
19	34.06167430587647	36.0	36.0	36.0	32.0	36.0
20	34.04608912786074	36.0	36.0	36.0	32.0	36.0
21	34.03620509443729	36.0	36.0	36.0	32.0	36.0
22	34.00817636757061	36.0	36.0	36.0	32.0	36.0
23	33.97034393302618	36.0	36.0	36.0	32.0	36.0
24	33.94258990037624	36.0	36.0	36.0	32.0	36.0
25	33.59442935995256	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	18.0
5	87.0
6	211.0
7	49.0
8	180.0
9	116.0
10	80.0
11	24.0
12	52.0
13	37.0
14	164.0
15	269.0
16	379.0
17	548.0
18	712.0
19	1013.0
20	1492.0
21	2129.0
22	3217.0
23	4621.0
24	6455.0
25	8929.0
26	12243.0
27	15609.0
28	20431.0
29	27264.0
30	35532.0
31	47924.0
32	67473.0
33	95354.0
34	208330.0
35	447451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24650803016705	17.314418600038675	11.47428809432905	25.964785275465225
2	16.71469683476895	19.384784122079264	37.14183146666878	26.758687576483002
3	19.22845021648456	22.737820636145287	28.212906565541367	29.820822581828782
4	12.712751133366856	15.461990183272686	35.25551955361561	36.569739129744846
5	14.940598302468524	36.245749227222404	33.45700420216418	15.356648268144898
6	34.84890884120444	35.066326895139206	16.357260234823407	13.727504028832948
7	30.70263304977311	30.227109601701958	19.94865538051307	19.121601968011863
8	28.118773349165018	33.141294776041605	18.972899828545277	19.7670320462481
9	27.1274250878691	14.404972298893941	18.473261978990845	39.994340634246115
10	16.140762358511267	26.873301365659806	31.305613673081716	25.680322602747207
11	37.17930257153781	21.362185136819317	21.835081952561357	19.623430339081516
12	24.240483389685767	23.778443084042085	28.699425552725522	23.28164797354663
13	29.350500539164386	19.63402355641963	24.966686988761946	26.048788915654036
14	23.348976757254235	19.653031703498375	25.01471845311788	31.98327308612951
15	25.1762813351803	27.084220822100086	22.076549940172086	25.66294790254753
16	25.726038495678022	26.079203477438945	23.36925548413488	24.825502542748154
17	24.021181463340042	26.080247679740086	24.96854852562836	24.93002233129151
18	24.713354043125214	25.437402519525882	25.668029780005618	24.18121365734329
19	25.477462954433555	25.286378508012643	25.213716786048952	24.022441751504854
20	25.57724719937585	24.59844320435474	24.9487321505498	24.875577445719607
21	26.28449650952845	24.342775919964822	24.722146950192414	24.650580620314315
22	25.588905379044707	24.497907845949957	25.038045933080856	24.875140841924484
23	24.441325811561352	24.614515802470116	25.401562580640043	25.542595805328496
24	24.499350863331916	25.194542487682288	25.21707368395977	25.089032965026025
25	24.62436799132905	24.774640645874648	25.279751305704607	25.3212400570917
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	551.0
1	551.0
2	473.5
3	396.0
4	396.0
5	396.0
6	867.0
7	1338.0
8	1338.0
9	1338.0
10	1523.5
11	1709.0
12	1709.0
13	1709.0
14	2294.5
15	2880.0
16	2880.0
17	2880.0
18	4754.0
19	6628.0
20	6628.0
21	6628.0
22	10521.0
23	14414.0
24	14414.0
25	14414.0
26	20445.5
27	26477.0
28	26477.0
29	26477.0
30	33365.5
31	40254.0
32	40254.0
33	40254.0
34	50468.0
35	60682.0
36	60682.0
37	60682.0
38	70535.5
39	80389.0
40	80389.0
41	80389.0
42	93247.5
43	106106.0
44	106106.0
45	106106.0
46	117664.0
47	129222.0
48	129222.0
49	129222.0
50	134326.0
51	139430.0
52	139430.0
53	139430.0
54	131149.0
55	122868.0
56	122868.0
57	122868.0
58	112760.0
59	102652.0
60	102652.0
61	102652.0
62	89723.0
63	76794.0
64	76794.0
65	76794.0
66	62392.5
67	47991.0
68	47991.0
69	47991.0
70	36187.5
71	24384.0
72	24384.0
73	24384.0
74	18133.5
75	11883.0
76	11883.0
77	11883.0
78	9350.5
79	6818.0
80	6818.0
81	6818.0
82	4644.0
83	2470.0
84	2470.0
85	2470.0
86	1802.0
87	1134.0
88	1134.0
89	1134.0
90	781.5
91	429.0
92	429.0
93	429.0
94	272.5
95	116.0
96	116.0
97	116.0
98	247.5
99	379.0
100	379.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.925082854519166E-4
2	0.0
3	7.933406981794814E-4
4	0.011404272536330046
5	0.033915314847172834
6	0.06525227242526235
7	0.10739849701604731
8	0.1121585412051242
9	0.12038945094873632
10	0.13238872900870097
11	0.13972713046686117
12	0.13278539935779068
13	0.12693451170871703
14	0.11453856329966264
15	0.13278539935779068
16	0.12058778612328118
17	0.12762868481962408
18	0.11315021707784854
19	0.0977792410506211
20	0.09321753203608907
21	0.09292002927427176
22	0.10501847492150887
23	0.0823090974361212
24	0.08944916371973653
25	0.08845748784701218
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1008394.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.22500652654053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86521217168388	45.33702486886757
2	13.56797958478045	15.799913997516207
3	3.9860371872181464	6.962611237224295
4	1.6042100442810143	3.7362056119281575
5	0.7823382246773046	2.2775824118899104
6	0.47063147006105854	1.64415122495403
7	0.3200180934510955	1.3043138905860765
8	0.2115459043301975	0.9853809328230936
9	0.170044270545705	0.8910745886092049
>10	0.876078021067268	9.35797982700742
>50	0.08338317554538337	3.3962383918281747
>100	0.058251234553833416	6.460202293022934
>500	0.003928968380278223	1.5161099333448007
>1k	3.4164942437201937E-4	0.3312107903980966
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2090	0.20726025739938952	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1240	0.12296780821781962	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.9667034908974074E-4
2	0.0	0.0	0.0	0.0	3.9667034908974074E-4
3	0.0	0.0	0.0	0.0	3.9667034908974074E-4
4	0.0	0.0	0.0	0.0	3.9667034908974074E-4
5	0.0	0.0	0.0	0.0	3.9667034908974074E-4
6	0.0	0.0	0.0	0.0	4.958379363621759E-4
7	0.0	0.0	0.0	0.0	4.958379363621759E-4
8	0.0	0.0	0.0	0.0	5.95005523634611E-4
9	0.0	0.0	0.0	0.0	5.95005523634611E-4
10	0.0	0.0	0.0	0.0	5.95005523634611E-4
11	0.0	0.0	0.0	0.0	5.95005523634611E-4
12	0.0	0.0	0.0	0.0	0.001090843459996787
13	0.0	0.0	0.0	0.0	0.001090843459996787
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTCA	40	0.005274838	14.251041	9
CGTTATT	80	1.2880628E-7	14.24892	2
GTATACT	55	1.9595101E-4	13.817821	4
GCGTTAT	90	3.6419806E-8	13.719821	1
CGCATCG	135	1.8189894E-12	13.371349	13
GCCTATT	50	0.0014997647	13.298993	2
AATAGGC	50	0.0014997647	13.298993	2
TATACTA	65	5.4501103E-5	13.153503	5
CGTCGTA	110	2.73576E-9	12.956136	10
CCGTCGT	110	2.737579E-9	12.955493	9
GGTTCTA	125	1.0186341E-10	12.920945	13
CCGACCA	165	0.0	12.667593	9
GGTATCA	465	0.0	12.255919	1
ACAATAC	70	1.0909376E-4	12.213361	3
GTTCTAG	70	1.0919488E-4	12.212149	1
GTATCAA	1145	0.0	12.111418	1
GTACCGG	55	0.0030711815	12.088794	1
CGGTCCA	150	1.4551915E-11	12.03481	10
CGACCAT	160	3.6379788E-12	11.876458	10
TCCCGGA	120	9.997166E-9	11.87469	17
>>END_MODULE