FastQCFastQC Report
Thu 2 Feb 2017
SRR4062302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008394
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10130.10045676590697684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA1350.024.4433982
GTATCAA11550.023.4298921
CACGCGA500.002580042222.00014929
CGTCTTA904.080157E-722.00014715
TTATACC1600.021.9990584
ATACCTT1750.021.3705126
ATCGTAA755.4691074E-520.534491
TTAGTAC550.00448421619.9991443
TGTACGC550.00448421619.99914410
CCCTTAT1452.9467628E-1019.725251
TCTGCGA1705.456968E-1219.41189618
ATACTAG708.124384E-418.8563376
CGAATGA1404.081812E-918.85633722
TCGGAAA600.007410980318.33345818
ACCGATC600.007413100518.332558
GGTATCA6500.018.2779521
TATACCT2050.018.2431225
GTATAGA1254.847643E-717.6009921
CCTAATA1254.852682E-717.5992472
GTAATCT750.001291039517.5992453
Produced by FastQC (version 0.11.5)