FastQCFastQC Report
Thu 2 Feb 2017
SRR4062301_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062301_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557621
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26000.16692122152949915No Hit
TATCAACGCAGAGTACTTTTTTTTT20490.13154676265920914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA400.005275374314.25151910
GGTATCA6650.012.9959551
CCGCGTC701.088837E-412.2167659
CCGTATG658.0270745E-411.69167514
ACCGTAT658.0270745E-411.69167513
GTATCAA14600.011.4484911
GTATGCG752.0754802E-411.39901616
TATGCGA752.0767194E-411.39828517
GTATAGG2000.011.3964531
TAGGACA1104.9586924E-711.228834
CTATTAG855.339535E-511.1729941
CGCCCAA600.005871006311.08451410
ACGCAAA600.005875221511.08344611
TTAGCCT951.3579962E-511.0015274
CAAGTTA700.001489280110.8589976
GTAGCGC700.00149007910.85833
TGTCGCC700.001492478110.85620615
TAATACA1855.456968E-1210.7852794
TTAGACA1502.1263986E-910.7681614
TAAATCA1158.7989247E-710.7406214
Produced by FastQC (version 0.11.5)