##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062301_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1557621
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.239967874084904	32.0	32.0	32.0	32.0	32.0
2	30.942261307468247	32.0	32.0	32.0	32.0	32.0
3	30.953208129577092	32.0	32.0	32.0	32.0	32.0
4	30.93448085253088	32.0	32.0	32.0	32.0	32.0
5	30.92460617826801	32.0	32.0	32.0	32.0	32.0
6	34.40014226824112	36.0	36.0	36.0	32.0	36.0
7	34.390834484126756	36.0	36.0	36.0	32.0	36.0
8	34.37173869638378	36.0	36.0	36.0	32.0	36.0
9	34.481386678787715	36.0	36.0	36.0	32.0	36.0
10	34.28496405736697	36.0	36.0	36.0	32.0	36.0
11	34.42386048981106	36.0	36.0	36.0	32.0	36.0
12	34.34774633880771	36.0	36.0	36.0	32.0	36.0
13	34.37422453857517	36.0	36.0	36.0	32.0	36.0
14	34.33168145524489	36.0	36.0	36.0	32.0	36.0
15	34.28534219813421	36.0	36.0	36.0	32.0	36.0
16	34.28081670701666	36.0	36.0	36.0	32.0	36.0
17	34.25635375999681	36.0	36.0	36.0	32.0	36.0
18	34.23309970782366	36.0	36.0	36.0	32.0	36.0
19	34.22276022215931	36.0	36.0	36.0	32.0	36.0
20	34.20849359375612	36.0	36.0	36.0	32.0	36.0
21	34.195267654968696	36.0	36.0	36.0	32.0	36.0
22	34.15903098378874	36.0	36.0	36.0	32.0	36.0
23	34.141275701855584	36.0	36.0	36.0	32.0	36.0
24	34.105761285961094	36.0	36.0	36.0	32.0	36.0
25	33.64418109411724	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	18.0
5	111.0
6	348.0
7	64.0
8	265.0
9	202.0
10	116.0
11	43.0
12	62.0
13	54.0
14	314.0
15	512.0
16	770.0
17	1070.0
18	1413.0
19	1765.0
20	2222.0
21	2865.0
22	3743.0
23	5002.0
24	6858.0
25	9638.0
26	13378.0
27	18072.0
28	25047.0
29	34707.0
30	49015.0
31	71217.0
32	110087.0
33	169351.0
34	389402.0
35	639890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.83443138876299	17.384030066730784	11.734833417008966	26.046705127497265
2	16.57582282690621	18.94386171705771	38.15062486437625	26.329690591659826
3	19.25359010179659	22.440190650327168	28.31943174697743	29.98678750089881
4	13.320611936839036	15.257072156899506	34.297240223155214	37.125075683106246
5	14.951885161466	36.13660709469092	33.69535061151828	15.216157132324797
6	35.33989875629561	34.08443827731524	16.299401274540035	14.27626169184911
7	30.761108401183286	29.99787276021252	19.951773995875342	19.289244842728856
8	28.112100063818836	34.09975918512411	18.572566127687796	19.21557462336926
9	26.710364867983056	14.982424093229113	18.732529342990816	39.57468169579702
10	16.9865113052914	27.446276321854153	31.06803231424037	24.49918005861408
11	36.555702844447076	21.586837087485744	21.704996779216238	20.152463288850946
12	23.659810315767547	23.882934204487164	28.917010255599312	23.540245224145977
13	29.996644584346278	19.857504110705577	24.596418061649654	25.54943324329849
14	23.1060438073679	19.146039771007235	25.509092416590985	32.238824005033884
15	25.15381238071545	26.88333786526222	22.05358732705549	25.90926242696684
16	25.35465380479531	26.051845562416563	23.454361160370816	25.139139472417316
17	23.876479424485957	26.202228240014346	25.18670974965885	24.734582585840847
18	24.278914441506558	25.95554521510774	25.41284309327355	24.35269725011215
19	25.54007342569488	25.30770224357718	25.1109964392458	24.041227891482144
20	25.529281660029827	24.837345530464017	25.280922341607482	24.352450467898677
21	25.48550770270774	24.736056794537483	25.052274570333232	24.726160932421543
22	25.677098194661873	24.344623444460726	25.11574163331303	24.862536727564375
23	24.82335532677578	24.69497993090345	25.283849051609092	25.19781569071167
24	24.514464535862537	24.96150929817121	25.29250363059208	25.231522535374175
25	24.639248457042985	24.803423581459455	25.478308642808262	25.079019318689298
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	81.0
2	270.0
3	459.0
4	459.0
5	459.0
6	952.0
7	1445.0
8	1445.0
9	1445.0
10	1859.0
11	2273.0
12	2273.0
13	2273.0
14	3296.0
15	4319.0
16	4319.0
17	4319.0
18	7239.5
19	10160.0
20	10160.0
21	10160.0
22	14952.0
23	19744.0
24	19744.0
25	19744.0
26	28393.5
27	37043.0
28	37043.0
29	37043.0
30	48319.5
31	59596.0
32	59596.0
33	59596.0
34	74604.0
35	89612.0
36	89612.0
37	89612.0
38	108370.5
39	127129.0
40	127129.0
41	127129.0
42	146706.5
43	166284.0
44	166284.0
45	166284.0
46	185725.5
47	205167.0
48	205167.0
49	205167.0
50	213019.5
51	220872.0
52	220872.0
53	220872.0
54	212610.0
55	204348.0
56	204348.0
57	204348.0
58	185431.0
59	166514.0
60	166514.0
61	166514.0
62	141385.5
63	116257.0
64	116257.0
65	116257.0
66	92932.0
67	69607.0
68	69607.0
69	69607.0
70	51487.0
71	33367.0
72	33367.0
73	33367.0
74	23727.0
75	14087.0
76	14087.0
77	14087.0
78	9884.0
79	5681.0
80	5681.0
81	5681.0
82	3877.5
83	2074.0
84	2074.0
85	2074.0
86	1384.5
87	695.0
88	695.0
89	695.0
90	454.0
91	213.0
92	213.0
93	213.0
94	148.0
95	83.0
96	83.0
97	83.0
98	297.0
99	511.0
100	511.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012198089265617247
2	1.926014094571144E-4
3	3.2100234909519066E-4
4	0.008988065774665339
5	0.032934841017166566
6	0.06298066089247642
7	0.10361955828792756
8	0.10618757708068909
9	0.11543244473463056
10	0.1298133499740951
11	0.13475678615016107
12	0.12730953165115264
13	0.12371430534128648
14	0.11324962876078327
15	0.12788733587952397
16	0.11607444943282094
17	0.1196696757426871
18	0.10933340010182194
19	0.09565870003036682
20	0.0903942615052057
21	0.09084366479393896
22	0.10073053714607083
23	0.0801221863341596
24	0.08994485821647243
25	0.08705583707461571
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1557621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.42384614779214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.07410523349591	28.464181929502658
2	16.616213731823564	14.763122447627222
3	7.248151860128448	9.659723492705387
4	3.7861157745675937	6.727752986684786
5	2.2072384286765856	4.902701018351157
6	1.422920095199597	3.7926950033849187
7	0.9621039384169664	2.9918250136894136
8	0.6839145452070744	2.4305691627613046
9	0.4890645013129128	1.9553513546404375
>10	2.3689944554664972	17.939409942360925
>50	0.10487146884322407	3.1284377033646735
>100	0.03441812395366182	2.443832969118206
>500	0.0014521868522219	0.40506912316467925
>1k	4.3565605566657007E-4	0.3953278526442354
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2600	0.16692122152949915	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2049	0.13154676265920914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.2840093963807628E-4
13	0.0	0.0	0.0	0.0	1.926014094571144E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	40	0.0052753743	14.251519	10
GGTATCA	665	0.0	12.995955	1
CCGCGTC	70	1.088837E-4	12.216765	9
CCGTATG	65	8.0270745E-4	11.691675	14
ACCGTAT	65	8.0270745E-4	11.691675	13
GTATCAA	1460	0.0	11.448491	1
GTATGCG	75	2.0754802E-4	11.399016	16
TATGCGA	75	2.0767194E-4	11.398285	17
GTATAGG	200	0.0	11.396453	1
TAGGACA	110	4.9586924E-7	11.22883	4
CTATTAG	85	5.339535E-5	11.172994	1
CGCCCAA	60	0.0058710063	11.084514	10
ACGCAAA	60	0.0058752215	11.083446	11
TTAGCCT	95	1.3579962E-5	11.001527	4
CAAGTTA	70	0.0014892801	10.858997	6
GTAGCGC	70	0.001490079	10.8583	3
TGTCGCC	70	0.0014924781	10.856206	15
TAATACA	185	5.456968E-12	10.785279	4
TTAGACA	150	2.1263986E-9	10.768161	4
TAAATCA	115	8.7989247E-7	10.740621	4
>>END_MODULE