FastQCFastQC Report
Thu 2 Feb 2017
SRR4062301_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062301_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557621
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16900.027.2117441
GGTATCA7850.027.1893561
CGTAGAC755.474856E-520.5328853
CGGATCG1303.5901394E-818.61497926
CGTTATT851.4303527E-418.11899644
ATCGATT1355.5299097E-817.92553329
TATCAAC24700.017.8139482
TCAACGC25000.017.5116164
CAACGCA25300.017.3039685
ATCAACG25350.017.2698383
AACGCAG26050.016.8057756
GGCGTTA1105.619334E-516.00119242
ACCGTAT850.002980891415.5290728
GTATAGG2850.015.4412331
AACACCG1057.161652E-414.6663475
TAGACAG2701.8189894E-1214.6663465
CGATTTT1503.2525095E-614.66634631
TAGACTG2451.0004442E-1014.3670335
GGATCGA2153.5470293E-914.32526827
GTCTTAG2803.6379788E-1214.1452721
Produced by FastQC (version 0.11.5)