##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062301_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1557621
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15953174745333	32.0	32.0	32.0	32.0	32.0
2	31.16622914046485	32.0	32.0	32.0	32.0	32.0
3	31.41014983747651	32.0	32.0	32.0	32.0	32.0
4	31.539855972666007	32.0	32.0	32.0	32.0	32.0
5	31.471961407813584	32.0	32.0	32.0	32.0	32.0
6	35.100557837882256	36.0	36.0	36.0	36.0	36.0
7	35.108577118567354	36.0	36.0	36.0	36.0	36.0
8	35.050669578799976	36.0	36.0	36.0	36.0	36.0
9	35.18885338602908	36.0	36.0	36.0	36.0	36.0
10	35.019147790123526	36.0	36.0	36.0	36.0	36.0
11	35.18353437710457	36.0	36.0	36.0	36.0	36.0
12	35.093113793406744	36.0	36.0	36.0	36.0	36.0
13	35.13387788171834	36.0	36.0	36.0	36.0	36.0
14	35.09004757896818	36.0	36.0	36.0	36.0	36.0
15	35.04838468407912	36.0	36.0	36.0	36.0	36.0
16	35.07651604594442	36.0	36.0	36.0	36.0	36.0
17	35.05902462794223	36.0	36.0	36.0	36.0	36.0
18	35.05169550230769	36.0	36.0	36.0	36.0	36.0
19	35.04404344830996	36.0	36.0	36.0	36.0	36.0
20	35.04956533071909	36.0	36.0	36.0	36.0	36.0
21	35.040630551334374	36.0	36.0	36.0	36.0	36.0
22	35.014844432631556	36.0	36.0	36.0	36.0	36.0
23	34.98979340930817	36.0	36.0	36.0	36.0	36.0
24	34.970276466483185	36.0	36.0	36.0	32.0	36.0
25	34.95369476913832	36.0	36.0	36.0	32.0	36.0
26	34.90718217075913	36.0	36.0	36.0	32.0	36.0
27	34.88794449997785	36.0	36.0	36.0	32.0	36.0
28	34.8716433586861	36.0	36.0	36.0	32.0	36.0
29	34.85643811941416	36.0	36.0	36.0	32.0	36.0
30	34.831374255996806	36.0	36.0	36.0	32.0	36.0
31	34.83344728916726	36.0	36.0	36.0	32.0	36.0
32	34.80211039784389	36.0	36.0	36.0	32.0	36.0
33	34.77345451814016	36.0	36.0	36.0	32.0	36.0
34	34.76981756152492	36.0	36.0	36.0	32.0	36.0
35	34.758738486448244	36.0	36.0	36.0	32.0	36.0
36	34.73433267784654	36.0	36.0	36.0	32.0	36.0
37	34.72624277664464	36.0	36.0	36.0	32.0	36.0
38	34.709381165251365	36.0	36.0	36.0	32.0	36.0
39	34.69275837960582	36.0	36.0	36.0	32.0	36.0
40	34.673719730280986	36.0	36.0	36.0	32.0	36.0
41	34.65045540603266	36.0	36.0	36.0	32.0	36.0
42	34.61997880100487	36.0	36.0	36.0	32.0	36.0
43	34.60625145654816	36.0	36.0	36.0	32.0	36.0
44	34.584097158423006	36.0	36.0	36.0	32.0	36.0
45	34.553659715681796	36.0	36.0	36.0	32.0	36.0
46	34.542460585726566	36.0	36.0	36.0	32.0	36.0
47	34.50645246821916	36.0	36.0	36.0	32.0	36.0
48	34.4910064771854	36.0	36.0	36.0	32.0	36.0
49	34.46166236844521	36.0	36.0	36.0	32.0	36.0
50	33.99082382684877	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	12.0
21	31.0
22	71.0
23	221.0
24	634.0
25	1610.0
26	3638.0
27	6993.0
28	12388.0
29	20787.0
30	32588.0
31	50366.0
32	79475.0
33	140395.0
34	313705.0
35	894702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.48884297971784	17.94775302293054	12.251048529361713	26.31235546798991
2	16.114743941626458	19.468140771914957	37.58069538475367	26.836419901704918
3	18.801966334514407	22.89796156826541	28.552361007409388	29.747711089810796
4	13.169579229850669	15.827095183677661	34.352730447734366	36.6505951387373
5	14.788459316136157	36.5144258548298	33.30119027747461	15.395924551559432
6	35.60421386744769	34.25555286626575	16.052898687034507	14.087334579252051
7	30.877536961815487	30.012692432883227	19.819070235955987	19.290700369345302
8	28.243199083730897	34.226875472274706	18.327564921120093	19.202360522874308
9	26.94878921123945	14.710382050575847	18.527035780847847	39.813792957336865
10	16.963369137935352	27.450387481935596	31.138576072099696	24.44766730802936
11	36.94666417568844	21.393137354979164	21.533928985292313	20.126269484040087
12	23.765922519020993	23.713727537058116	28.911461774077264	23.608888169843627
13	30.148027023261758	19.688807482693157	24.615551536606144	25.54761395743894
14	23.185807073736164	18.928673920035745	25.376006101612653	32.509512904615434
15	25.390900610610668	26.790856055484614	21.905778106484185	25.912465227420533
16	25.519319267393247	25.869854957832995	23.417196536245132	25.193629238528626
17	24.08808047657293	26.076112224989263	25.027846953784007	24.8079603446538
18	24.58057806140137	25.677700594496734	25.27689680409856	24.464824540003338
19	25.724614652730025	25.26262807191223	24.9789261957819	24.03383107957584
20	25.805461679900567	24.68349066778975	25.1020790746885	24.408968577621184
21	25.79677598080663	24.644377547554893	24.847828836411427	24.711017635227055
22	25.8813279995583	24.368829131091584	24.944065340670164	24.805777528679954
23	25.08504957239277	24.571445813840466	25.13249371958904	25.211010894177722
24	24.80381765105062	24.858837948211583	25.085724447822844	25.25161995291495
25	25.12893109016028	24.499443058778965	25.333602976345244	25.03802287471551
26	24.85580528191801	24.91332908752863	25.11530441392487	25.11556121662849
27	25.08434380041769	25.1376304640498	24.818038883856257	24.95998685167625
28	25.434128315027436	24.53466991610191	25.00442988076559	25.026771888105063
29	24.94732327778256	25.09671844243824	24.949377702049418	25.006580577729785
30	25.125336815607056	24.93331099131165	24.908721927217652	25.032630265863638
31	25.100377885663583	24.889221591485438	24.852498757715328	25.157901765135648
32	25.098596631118593	25.13583319048619	24.825357326517757	24.94021285187746
33	25.12854555574887	24.976197645252142	24.724916683733316	25.17034011526567
34	25.08604545305716	24.869687925131306	25.204688987658635	24.839577634152903
35	25.27850181687446	24.849706603664565	25.217703932923307	24.65408764653767
36	25.22828140678999	24.861053776377634	25.07978533875786	24.830879478074518
37	25.324475215589192	24.763802073970588	24.96411126918988	24.947611441250338
38	25.271616137686898	24.973469155847283	25.01115483163106	24.743759874834765
39	25.31245004362456	24.975844387134384	24.862979091671917	24.848726477569148
40	25.53714928085844	24.81341738458842	24.689831480186772	24.95960185436637
41	24.958477972941825	25.056063564172476	24.91873752493408	25.066720937951615
42	25.131023501728684	24.881014673733343	25.106369318382455	24.88159250615552
43	25.16907834166473	24.6529512147831	24.93313267786451	25.244837765687656
44	24.88886434901859	24.999807390552892	25.169753126051326	24.941575134377192
45	25.071970436265396	24.898304040103135	24.95159169585717	25.078133827774295
46	25.18822407765868	24.87427652423463	24.831774830908103	25.10572456719858
47	25.01298476176413	25.13959020156074	25.05953087933641	24.78789415733872
48	24.985185304078456	25.2425725887999	24.77318909201804	24.999053015103605
49	25.101183383325797	24.96423912150224	24.878207564613554	25.056369930558407
50	24.959135902496275	25.18384028782706	24.780913224302488	25.076110585374185
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	23.5
2	30.0
3	86.5
4	143.0
5	167.5
6	192.0
7	165.0
8	138.0
9	180.5
10	223.0
11	365.5
12	508.0
13	996.5
14	1485.0
15	1843.5
16	2202.0
17	2273.0
18	2344.0
19	2414.0
20	2484.0
21	3027.5
22	3571.0
23	4603.5
24	5636.0
25	6983.0
26	8330.0
27	10207.5
28	12085.0
29	14643.5
30	17202.0
31	20608.5
32	24015.0
33	27594.0
34	31173.0
35	36233.5
36	41294.0
37	46474.5
38	51655.0
39	58172.0
40	64689.0
41	70758.0
42	76827.0
43	81573.0
44	86319.0
45	93639.0
46	100959.0
47	106443.0
48	111927.0
49	116937.5
50	121948.0
51	125898.0
52	129848.0
53	130288.5
54	130729.0
55	127690.5
56	124652.0
57	118513.0
58	112374.0
59	102141.5
60	91909.0
61	79769.0
62	67629.0
63	57727.5
64	47826.0
65	40322.0
66	32818.0
67	26824.0
68	20830.0
69	16925.5
70	13021.0
71	10151.5
72	7282.0
73	5959.5
74	4637.0
75	3735.5
76	2834.0
77	2323.0
78	1812.0
79	1448.5
80	1085.0
81	798.5
82	512.0
83	369.5
84	227.0
85	154.5
86	82.0
87	60.0
88	38.0
89	28.5
90	19.0
91	21.0
92	23.0
93	18.5
94	14.0
95	13.5
96	13.0
97	11.5
98	10.0
99	5.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02824820672037678
2	0.0042372310080565165
3	1.2840093963807628E-4
4	6.420046981903814E-5
5	6.420046981903814E-5
6	1.2840093963807628E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.2100234909519066E-4
17	0.0
18	6.420046981903814E-5
19	0.0
20	3.2100234909519066E-4
21	0.0
22	0.0
23	0.0
24	8.988065774665339E-4
25	3.852028189142288E-4
26	3.2100234909519066E-4
27	5.136037585523051E-4
28	0.0010914079869236482
29	4.4940328873326697E-4
30	0.0011556084567426864
31	4.4940328873326697E-4
32	7.704056378284576E-4
33	1.2840093963807628E-4
34	8.988065774665339E-4
35	6.420046981903814E-5
36	2.5680187927615256E-4
37	0.0018618136247521059
38	0.0
39	7.704056378284576E-4
40	0.0
41	8.988065774665339E-4
42	0.004879235706246899
43	0.00378782771932325
44	0.004173030538237478
45	0.002375417383304411
46	0.002311216913485373
47	0.0016692122152949915
48	0.002953221611675754
49	0.003402624900409021
50	0.0012198089265617247
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1557621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.03294751151507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.19658593311243	29.551580706774317
2	16.639236037727915	15.319061583128782
3	7.429540456150119	10.260109375579084
4	3.7845270077796562	6.968517324201283
5	2.203803635681284	5.072378854350131
6	1.3804214746308674	3.8126921571270573
7	0.9187439588324589	2.96047447034193
8	0.6517643476087143	2.400210720267905
9	0.4810731174400331	1.9930692207886174
>10	2.2077349055927242	17.33223594583258
>50	0.08272128334617022	2.5809279779859366
>100	0.02356729611383228	1.6603428794263193
>500	2.805459836187579E-4	0.0883987841959548
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.926014094571144E-4
2	0.0	0.0	0.0	0.0	1.926014094571144E-4
3	0.0	0.0	0.0	0.0	1.926014094571144E-4
4	0.0	0.0	0.0	0.0	1.926014094571144E-4
5	0.0	0.0	0.0	0.0	1.926014094571144E-4
6	0.0	0.0	0.0	0.0	1.926014094571144E-4
7	0.0	0.0	0.0	0.0	1.926014094571144E-4
8	6.420046981903814E-5	0.0	0.0	0.0	1.926014094571144E-4
9	6.420046981903814E-5	0.0	0.0	0.0	1.926014094571144E-4
10	6.420046981903814E-5	0.0	0.0	0.0	1.926014094571144E-4
11	6.420046981903814E-5	0.0	0.0	0.0	1.926014094571144E-4
12	6.420046981903814E-5	0.0	0.0	0.0	2.5680187927615256E-4
13	6.420046981903814E-5	0.0	0.0	0.0	3.852028189142288E-4
14	6.420046981903814E-5	0.0	0.0	0.0	3.852028189142288E-4
15	6.420046981903814E-5	0.0	0.0	6.420046981903814E-5	4.4940328873326697E-4
16	6.420046981903814E-5	0.0	0.0	2.5680187927615256E-4	4.4940328873326697E-4
17	6.420046981903814E-5	0.0	0.0	2.5680187927615256E-4	4.4940328873326697E-4
18	6.420046981903814E-5	0.0	0.0	2.5680187927615256E-4	4.4940328873326697E-4
19	6.420046981903814E-5	0.0	0.0	2.5680187927615256E-4	4.4940328873326697E-4
20	6.420046981903814E-5	0.0	0.0	3.852028189142288E-4	5.136037585523051E-4
21	6.420046981903814E-5	0.0	0.0	5.136037585523051E-4	5.778042283713432E-4
22	6.420046981903814E-5	0.0	0.0	6.420046981903813E-4	5.778042283713432E-4
23	6.420046981903814E-5	0.0	0.0	8.346061076474957E-4	5.778042283713432E-4
24	6.420046981903814E-5	0.0	0.0	0.0013482098661998009	5.778042283713432E-4
25	6.420046981903814E-5	0.0	0.0	0.0017976131549330679	5.778042283713432E-4
26	6.420046981903814E-5	0.0	0.0	0.0025680187927615253	5.778042283713432E-4
27	6.420046981903814E-5	0.0	0.0	0.0031458230211328687	5.778042283713432E-4
28	6.420046981903814E-5	0.0	0.0	0.0037236272495042117	5.778042283713432E-4
29	6.420046981903814E-5	0.0	0.0	0.004301431477875555	5.778042283713432E-4
30	6.420046981903814E-5	0.0	0.0	0.006355846512084776	5.778042283713432E-4
31	6.420046981903814E-5	0.0	0.0	0.009951072821950912	5.778042283713432E-4
32	6.420046981903814E-5	0.0	0.0	0.0138031010110932	7.062051680094195E-4
33	6.420046981903814E-5	0.0	0.0	0.0178477306096926	7.062051680094195E-4
34	6.420046981903814E-5	0.0	0.0	0.02202076114793008	7.062051680094195E-4
35	6.420046981903814E-5	0.0	0.0	0.02715679873345313	7.062051680094195E-4
36	6.420046981903814E-5	0.0	0.0	0.034475652292823476	7.062051680094195E-4
37	6.420046981903814E-5	0.0	0.0	0.04551813310169804	7.062051680094195E-4
38	6.420046981903814E-5	0.0	0.0	0.0592570336429722	7.062051680094195E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1690	0.0	27.211744	1
GGTATCA	785	0.0	27.189356	1
CGTAGAC	75	5.474856E-5	20.532885	3
CGGATCG	130	3.5901394E-8	18.614979	26
CGTTATT	85	1.4303527E-4	18.118996	44
ATCGATT	135	5.5299097E-8	17.925533	29
TATCAAC	2470	0.0	17.813948	2
TCAACGC	2500	0.0	17.511616	4
CAACGCA	2530	0.0	17.303968	5
ATCAACG	2535	0.0	17.269838	3
AACGCAG	2605	0.0	16.805775	6
GGCGTTA	110	5.619334E-5	16.001192	42
ACCGTAT	85	0.0029808914	15.529072	8
GTATAGG	285	0.0	15.441233	1
AACACCG	105	7.161652E-4	14.666347	5
TAGACAG	270	1.8189894E-12	14.666346	5
CGATTTT	150	3.2525095E-6	14.666346	31
TAGACTG	245	1.0004442E-10	14.367033	5
GGATCGA	215	3.5470293E-9	14.325268	27
GTCTTAG	280	3.6379788E-12	14.145272	1
>>END_MODULE