##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062300_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25872
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.271722325293755	32.0	32.0	32.0	32.0	32.0
2	31.553416821273963	32.0	32.0	32.0	32.0	32.0
3	31.60532622139765	32.0	32.0	32.0	32.0	32.0
4	31.670957019171304	32.0	32.0	32.0	32.0	32.0
5	31.697317563388992	32.0	32.0	32.0	32.0	32.0
6	35.33275355596784	36.0	36.0	36.0	36.0	36.0
7	35.31153370439085	36.0	36.0	36.0	36.0	36.0
8	35.29645949288806	36.0	36.0	36.0	36.0	36.0
9	35.3588048855906	36.0	36.0	36.0	36.0	36.0
10	35.24037569573284	36.0	36.0	36.0	36.0	36.0
11	35.35049474335189	36.0	36.0	36.0	36.0	36.0
12	35.31953463203463	36.0	36.0	36.0	36.0	36.0
13	35.30647031539889	36.0	36.0	36.0	36.0	36.0
14	35.300054112554115	36.0	36.0	36.0	36.0	36.0
15	35.28007111935683	36.0	36.0	36.0	36.0	36.0
16	35.29012059369202	36.0	36.0	36.0	36.0	36.0
17	35.30623840445269	36.0	36.0	36.0	36.0	36.0
18	35.28014842300556	36.0	36.0	36.0	36.0	36.0
19	35.278370439084725	36.0	36.0	36.0	36.0	36.0
20	35.28668058132344	36.0	36.0	36.0	36.0	36.0
21	35.237515460729746	36.0	36.0	36.0	36.0	36.0
22	35.237901978973404	36.0	36.0	36.0	36.0	36.0
23	35.20384972170687	36.0	36.0	36.0	36.0	36.0
24	35.20960884353742	36.0	36.0	36.0	36.0	36.0
25	35.21818954854669	36.0	36.0	36.0	36.0	36.0
26	35.156269325912184	36.0	36.0	36.0	36.0	36.0
27	35.12596629560915	36.0	36.0	36.0	36.0	36.0
28	35.112438157081016	36.0	36.0	36.0	36.0	36.0
29	35.08905380333952	36.0	36.0	36.0	36.0	36.0
30	35.05681818181818	36.0	36.0	36.0	36.0	36.0
31	35.06485776128633	36.0	36.0	36.0	36.0	36.0
32	35.06253865182437	36.0	36.0	36.0	36.0	36.0
33	35.056161100803955	36.0	36.0	36.0	36.0	36.0
34	35.026785714285715	36.0	36.0	36.0	36.0	36.0
35	35.00606833642548	36.0	36.0	36.0	36.0	36.0
36	34.98133116883117	36.0	36.0	36.0	36.0	36.0
37	34.99791280148423	36.0	36.0	36.0	36.0	36.0
38	34.93614718614719	36.0	36.0	36.0	36.0	36.0
39	34.93865955473098	36.0	36.0	36.0	36.0	36.0
40	34.96014996907854	36.0	36.0	36.0	36.0	36.0
41	34.893475572047	36.0	36.0	36.0	36.0	36.0
42	34.890267470624615	36.0	36.0	36.0	36.0	36.0
43	34.88845083487941	36.0	36.0	36.0	36.0	36.0
44	34.84241651205937	36.0	36.0	36.0	36.0	36.0
45	34.842455163883734	36.0	36.0	36.0	36.0	36.0
46	34.78594619666048	36.0	36.0	36.0	36.0	36.0
47	34.84744124922696	36.0	36.0	36.0	36.0	36.0
48	34.77141311069882	36.0	36.0	36.0	32.0	36.0
49	34.80229591836735	36.0	36.0	36.0	36.0	36.0
50	34.34117965367965	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	6.0
24	19.0
25	33.0
26	63.0
27	112.0
28	193.0
29	330.0
30	411.0
31	673.0
32	951.0
33	1523.0
34	3435.0
35	18119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.98082276523353	21.945561398082276	12.12496133622023	26.948654500463963
2	14.804793196752996	22.74449168921531	38.0827212988017	24.367993815229998
3	16.86765615337044	25.660946196660483	31.122448979591837	26.348948670377244
4	12.105751391465677	18.262987012987015	38.6209029066172	31.01035868893012
5	13.667285095856524	36.47186147186147	35.192486085343226	14.668367346938776
6	28.79560915275201	38.66341991341991	18.974180581323438	13.566790352504638
7	25.966295609152752	32.4095547309833	24.265615337043908	17.358534322820038
8	23.12925170068027	35.93460111317254	22.866419294990724	18.06972789115646
9	23.7012987012987	16.875386518243662	22.15909090909091	37.26422387136673
10	14.049938157081016	28.57142857142857	35.03014842300556	22.348484848484848
11	31.582405689548548	24.242424242424242	25.780766852195423	18.394403215831787
12	20.78308596165739	26.02040816326531	32.44047619047619	20.756029684601113
13	26.808905380333954	22.978509585652443	26.79730983302412	23.415275200989484
14	20.601422387136672	22.174551638837354	28.590754483611626	28.633271490414348
15	22.30983302411874	30.272108843537417	24.331323438466296	23.08673469387755
16	21.66821273964131	30.13296227581942	26.248453927025356	21.950371057513916
17	20.412028447742735	28.706709956709958	29.089363017934446	21.791898577612866
18	20.32312925170068	29.51453308596166	29.03138528138528	21.13095238095238
19	21.030457637600495	28.749226963512676	28.8265306122449	21.393784786641927
20	22.12816944959802	28.702844774273345	27.844774273345703	21.32421150278293
21	22.452844774273345	28.75309214594929	27.346165739022883	21.447897340754484
22	21.40538033395176	29.15120593692022	28.103741496598637	21.339672232529374
23	21.722325293753865	28.741496598639454	27.75587507730365	21.78030303030303
24	21.791898577612866	28.35884353741497	28.37430426716141	21.474953617810762
25	21.896258503401363	27.593537414965986	28.644867037724183	21.86533704390847
26	21.436301793444652	28.432282003710576	28.157854050711194	21.97356215213358
27	21.35899814471243	27.9143475572047	29.05844155844156	21.66821273964131
28	21.498975687062735	28.340613041629624	28.6807622434386	21.479649027869044
29	21.223716759431042	28.038033395176253	28.53277674706246	22.20547309833024
30	21.44016697588126	28.79560915275201	28.31246134817563	21.451762523191096
31	22.534013605442176	28.71444032158318	27.81385281385281	20.937693259121833
32	21.13095238095238	28.474799010513298	28.845856524427955	21.548392084106368
33	21.47881880024737	27.806122448979593	29.22850958565244	21.486549165120593
34	21.3474025974026	27.562615955473095	29.39471243042672	21.695269016697587
35	21.428571428571427	27.508503401360546	28.61394557823129	22.448979591836736
36	21.722325293753865	29.17439703153989	27.98778602350031	21.115491651205936
37	21.16573902288188	28.71444032158318	28.888373531230677	21.231447124304268
38	21.20439084724799	28.791743970315398	28.648732220160795	21.355132962275817
39	22.019944341372913	27.663110698824983	28.791743970315398	21.525200989486702
40	21.09230055658627	28.45547309833024	28.996598639455783	21.455627705627705
41	21.92717996289425	28.579158936301795	28.378169449598023	21.115491651205936
42	20.902906617192333	28.428416821273967	28.331787260358688	22.336889301175013
43	21.078469269424044	27.595670660997296	29.52454580595284	21.80131426362582
44	21.831394225194234	27.13462950794326	29.496347261412392	21.537629005450118
45	22.058596165739022	27.106524427952998	28.683518862090292	22.151360544217688
46	21.378324056895483	28.61394557823129	28.424551638837354	21.583178726035868
47	21.459492888064315	28.235157699443413	28.72990105132962	21.575448361162646
48	20.65940012368584	28.21583178726036	28.9695423623995	22.155225726654297
49	21.140316969462695	27.634325473521454	29.3931194433707	21.83223811364515
50	20.902906617192333	28.273809523809522	28.55596784168213	22.267316017316016
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	1.5
8	1.0
9	2.5
10	4.0
11	6.5
12	9.0
13	16.5
14	24.0
15	28.5
16	33.0
17	51.0
18	69.0
19	114.0
20	159.0
21	206.5
22	254.0
23	325.0
24	396.0
25	462.5
26	529.0
27	653.5
28	778.0
29	892.5
30	1007.0
31	1137.0
32	1267.0
33	1377.5
34	1488.0
35	1600.0
36	1712.0
37	1776.0
38	1840.0
39	1835.5
40	1831.0
41	1815.5
42	1800.0
43	1715.5
44	1631.0
45	1551.5
46	1472.0
47	1420.5
48	1369.0
49	1323.0
50	1277.0
51	1210.0
52	1143.0
53	1117.5
54	1092.0
55	1070.5
56	1049.0
57	982.5
58	916.0
59	836.5
60	757.0
61	672.0
62	587.0
63	521.0
64	455.0
65	390.0
66	325.0
67	272.5
68	220.0
69	179.0
70	138.0
71	110.0
72	82.0
73	62.0
74	42.0
75	42.5
76	43.0
77	31.5
78	20.0
79	23.0
80	26.0
81	18.5
82	11.0
83	8.0
84	5.0
85	5.0
86	5.0
87	3.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030921459492888066
2	0.007730364873222016
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.003865182436611008
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.007730364873222016
44	0.003865182436611008
45	0.0
46	0.0
47	0.0
48	0.0
49	0.007730364873222016
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	25872.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.03324056895485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.02989384634157	90.3099876314162
2	4.726074754951804	8.982683982683982
3	0.2318298287713019	0.6609461966604824
4	0.012201569935331679	0.0463821892393321
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.003865182436611008	0.0
28	0.0	0.0	0.0	0.003865182436611008	0.0
29	0.0	0.0	0.0	0.030921459492888066	0.0
30	0.0	0.0	0.0	0.06570810142238713	0.0
31	0.0	0.0	0.0	0.11595547309833024	0.0
32	0.0	0.0	0.0	0.20871985157699444	0.0
33	0.0	0.0	0.0	0.31307977736549164	0.0
34	0.0	0.0	0.0	0.3749226963512678	0.0
35	0.0	0.0	0.0	0.5140692640692641	0.0
36	0.0	0.0	0.0	0.6532158317872604	0.0
37	0.0	0.0	0.0	0.8271490414347558	0.0
38	0.0	0.0	0.0	1.0435992578849722	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE