FastQCFastQC Report
Thu 2 Feb 2017
SRR4062299_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062299_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2667212
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50900.19083597404330815No Hit
TATCAACGCAGAGTACTTTTTTTTT27430.10284146891960594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG3400.012.57365413
TCGCGTA2350.012.53321559
GTATCAA34250.012.339481
ACGAACG853.9466904E-612.29400915
CGCGTAA2400.012.27233710
GCGTAAC2400.012.27233711
ATCGCCA3900.012.17937916
AACCGCG1052.7188253E-711.7631257
GTGCGAA752.0725143E-411.40139811
CGACCAT4050.011.26063810
GGTATCA13050.011.2074721
TTAGACG600.00587709411.0834434
CGGTAAT600.005890247411.0801141
GTGCTAG2850.010.9968051
GCGTTAT1408.398274E-910.853991
GTATTAC2200.010.7923191
CGAGCCG4500.010.76657115
ACGGTAT3100.010.7269199
TCTATAC1701.382432E-1010.6175533
CCGACCA3950.010.5833769
Produced by FastQC (version 0.11.5)