##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062299_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2667212
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25395169187901	32.0	32.0	32.0	32.0	32.0
2	30.796051082553618	32.0	32.0	32.0	32.0	32.0
3	30.82301744293292	32.0	32.0	32.0	32.0	32.0
4	30.82897722415766	32.0	32.0	32.0	32.0	32.0
5	30.752910529796658	32.0	32.0	32.0	32.0	32.0
6	34.42024181054974	36.0	36.0	36.0	32.0	36.0
7	34.35443939214431	36.0	36.0	36.0	32.0	36.0
8	34.3058605765121	36.0	36.0	36.0	32.0	36.0
9	34.439994646094874	36.0	36.0	36.0	32.0	36.0
10	34.127551915633255	36.0	36.0	36.0	32.0	36.0
11	34.42758393408548	36.0	36.0	36.0	32.0	36.0
12	34.24844744249801	36.0	36.0	36.0	32.0	36.0
13	34.332587735808026	36.0	36.0	36.0	32.0	36.0
14	34.24016726079517	36.0	36.0	36.0	32.0	36.0
15	34.19370638704385	36.0	36.0	36.0	32.0	36.0
16	34.19590118820701	36.0	36.0	36.0	32.0	36.0
17	34.12981645253546	36.0	36.0	36.0	32.0	36.0
18	34.139038816562014	36.0	36.0	36.0	32.0	36.0
19	34.14187998554296	36.0	36.0	36.0	32.0	36.0
20	34.12927618801955	36.0	36.0	36.0	32.0	36.0
21	34.10440377442813	36.0	36.0	36.0	32.0	36.0
22	34.079906659088216	36.0	36.0	36.0	32.0	36.0
23	34.04331451718124	36.0	36.0	36.0	32.0	36.0
24	34.02979328227377	36.0	36.0	36.0	32.0	36.0
25	33.671460311366324	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	48.0
5	192.0
6	508.0
7	105.0
8	454.0
9	340.0
10	183.0
11	54.0
12	91.0
13	132.0
14	440.0
15	596.0
16	943.0
17	1270.0
18	1789.0
19	2376.0
20	3756.0
21	5393.0
22	8250.0
23	11900.0
24	17104.0
25	23191.0
26	31122.0
27	39973.0
28	51908.0
29	68191.0
30	88791.0
31	119901.0
32	170475.0
33	242925.0
34	541851.0
35	1232955.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.25738652745383	16.674571374260598	11.25207943799966	25.815962660285912
2	17.080001664662696	19.308887536662883	37.23220582429485	26.37890497437957
3	20.16354290470495	22.57210772385919	27.387100281569744	29.877249089866115
4	13.299019556378134	15.173816140948707	34.58227282339866	36.9448914792745
5	15.313683721000007	36.062114177274054	32.82937915825951	15.794822943466421
6	35.37000737182313	34.210248897509146	16.388669526608904	14.031074204058816
7	31.38058291270196	29.8040081112516	19.445198196849102	19.370210779197333
8	28.381282516482027	33.04200016664052	18.5617647080901	20.014952608787357
9	26.95007189853804	14.990028793196833	18.07995808076884	39.97994122749628
10	16.27317407926864	26.643208526919928	30.71122987425256	26.372387519558878
11	37.3587056256448	21.393473765462225	21.671444682886392	19.576375926006577
12	25.302085003136572	23.26566023597675	27.875499720880853	23.556755040005825
13	29.48713280355213	19.500785887583728	24.705475160734373	26.30660614812977
14	23.76200163650149	19.26008362672752	24.70115080586438	32.276763930906604
15	25.294671271235718	26.715497923776837	21.8832156850673	26.10661511992014
16	26.02874444886954	25.995299720693353	22.93505078831589	25.040905042121214
17	24.43620029895142	25.961775960612286	24.504932097787595	25.0970916426487
18	24.993122095657107	25.3066344284058	25.166936966117927	24.53330650981917
19	25.673309918644026	24.955370361266464	24.958485147909208	24.4128345721803
20	25.91114098120248	24.39125616868046	24.592353932063553	25.10524891805351
21	26.460523782845254	24.300241323915532	24.37998216056765	24.859252732671568
22	26.13836081446996	24.163246771036814	24.62095922208399	25.077433192409238
23	24.704498023733212	24.401547994353738	25.150593255253224	25.743360726659827
24	24.8563925754924	24.759015709383903	24.9902810757911	25.394310639332602
25	24.99288920442393	24.373368175606256	25.006360315831895	25.627382304137917
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	396.0
1	396.0
2	614.0
3	832.0
4	832.0
5	832.0
6	1660.5
7	2489.0
8	2489.0
9	2489.0
10	2972.5
11	3456.0
12	3456.0
13	3456.0
14	4284.5
15	5113.0
16	5113.0
17	5113.0
18	8649.0
19	12185.0
20	12185.0
21	12185.0
22	19694.5
23	27204.0
24	27204.0
25	27204.0
26	42615.5
27	58027.0
28	58027.0
29	58027.0
30	76778.0
31	95529.0
32	95529.0
33	95529.0
34	121768.5
35	148008.0
36	148008.0
37	148008.0
38	180644.0
39	213280.0
40	213280.0
41	213280.0
42	248187.5
43	283095.0
44	283095.0
45	283095.0
46	316857.5
47	350620.0
48	350620.0
49	350620.0
50	364587.5
51	378555.0
52	378555.0
53	378555.0
54	359094.0
55	339633.0
56	339633.0
57	339633.0
58	311405.5
59	283178.0
60	283178.0
61	283178.0
62	246073.5
63	208969.0
64	208969.0
65	208969.0
66	168945.5
67	128922.0
68	128922.0
69	128922.0
70	97475.5
71	66029.0
72	66029.0
73	66029.0
74	49178.0
75	32327.0
76	32327.0
77	32327.0
78	24890.0
79	17453.0
80	17453.0
81	17453.0
82	12064.0
83	6675.0
84	6675.0
85	6675.0
86	4810.0
87	2945.0
88	2945.0
89	2945.0
90	1938.0
91	931.0
92	931.0
93	931.0
94	594.5
95	258.0
96	258.0
97	258.0
98	680.5
99	1103.0
100	1103.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.373083204484682E-4
2	1.8746166408969365E-4
3	8.24831321994652E-4
4	0.009598037201392315
5	0.031193620904525026
6	0.06212479547932448
7	0.10366630024160059
8	0.1047535778933208
9	0.11401418409935168
10	0.12972347155006803
11	0.13587221413220996
12	0.1302858565423371
13	0.12541185327600504
14	0.1121770597912727
15	0.13051081053924474
16	0.11678861672787914
17	0.12125020433321385
18	0.10809039551411737
19	0.09384330904330065
20	0.0876945664611587
21	0.08731964313297931
22	0.0996171282972632
23	0.07820900625822019
24	0.0865323041438026
25	0.08435774884036215
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2667212.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.61699929267518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4216147614777	37.16381822448786
2	14.67825386654834	14.859383311615682
3	5.0729269728762985	7.703290229936174
4	2.2365438079494466	4.528285453800568
5	1.2316929437590007	3.1172300431521176
6	0.7604934745891028	2.3096338599216426
7	0.4776230630509002	1.6923092371228936
8	0.3422959803437521	1.3860796315956205
9	0.2558910846860168	1.1657194967298599
>10	1.3655063042967077	12.382608265899513
>50	0.08790242912641834	3.0329606259065853
>100	0.05934486495499791	5.973844126825494
>500	0.007078890243797556	2.39997499813453
>1k	0.002757041463353553	2.0928825992164266
>5k	7.451463414469061E-5	0.19197989565520393
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5090	0.19083597404330815	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2743	0.10284146891960594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2495399690763239E-4
2	0.0	0.0	0.0	0.0	2.2495399690763239E-4
3	0.0	0.0	0.0	0.0	2.2495399690763239E-4
4	0.0	0.0	0.0	0.0	2.2495399690763239E-4
5	0.0	0.0	0.0	0.0	2.624463297255711E-4
6	0.0	0.0	0.0	0.0	3.749233281793873E-4
7	0.0	0.0	0.0	0.0	3.749233281793873E-4
8	0.0	0.0	0.0	0.0	3.749233281793873E-4
9	0.0	0.0	0.0	0.0	4.1241566099732606E-4
10	0.0	0.0	0.0	0.0	5.998773250870197E-4
11	0.0	0.0	0.0	0.0	5.998773250870197E-4
12	0.0	0.0	0.0	0.0	0.0011997546501740393
13	0.0	0.0	0.0	0.0	0.0012372469829919782
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	340	0.0	12.573654	13
TCGCGTA	235	0.0	12.5332155	9
GTATCAA	3425	0.0	12.33948	1
ACGAACG	85	3.9466904E-6	12.294009	15
CGCGTAA	240	0.0	12.272337	10
GCGTAAC	240	0.0	12.272337	11
ATCGCCA	390	0.0	12.179379	16
AACCGCG	105	2.7188253E-7	11.763125	7
GTGCGAA	75	2.0725143E-4	11.401398	11
CGACCAT	405	0.0	11.260638	10
GGTATCA	1305	0.0	11.207472	1
TTAGACG	60	0.005877094	11.083443	4
CGGTAAT	60	0.0058902474	11.080114	1
GTGCTAG	285	0.0	10.996805	1
GCGTTAT	140	8.398274E-9	10.85399	1
GTATTAC	220	0.0	10.792319	1
CGAGCCG	450	0.0	10.766571	15
ACGGTAT	310	0.0	10.726919	9
TCTATAC	170	1.382432E-10	10.617553	3
CCGACCA	395	0.0	10.583376	9
>>END_MODULE