FastQCFastQC Report
Thu 2 Feb 2017
SRR4062299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2667212
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34850.027.0239241
TAATCGG755.4778586E-520.53233515
GGTATCA15250.020.2006651
TCAACGC48850.019.2747574
CAACGCA49450.019.1298645
AACGCAG49850.019.0204966
ATCAACG49650.019.008143
TATCAAC50100.018.9262922
CTAGCGG4950.018.6668129
ATACGAA4850.018.59982340
CGCAATA4800.018.33381836
CAATACG5000.018.04149238
TACGAAT5050.017.86319741
TCTAGCG5200.017.34628728
CGAATGC5300.017.02123343
TAGCGGC5400.016.7038330
GTGTAGG5150.016.6634461
AATACGA5300.016.60545739
ACGCAGA59400.015.8884157
GTATAGG4200.015.7173531
Produced by FastQC (version 0.11.5)