##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062299_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2667212
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27950159192445	32.0	32.0	32.0	32.0	32.0
2	31.476796370142306	32.0	32.0	32.0	32.0	32.0
3	31.586095893389803	32.0	32.0	32.0	32.0	32.0
4	31.66115216938136	32.0	32.0	32.0	32.0	32.0
5	31.64201720748107	32.0	32.0	32.0	32.0	32.0
6	35.295553184373794	36.0	36.0	36.0	36.0	36.0
7	35.29612756691257	36.0	36.0	36.0	36.0	36.0
8	35.2550850851001	36.0	36.0	36.0	36.0	36.0
9	35.31691369115016	36.0	36.0	36.0	36.0	36.0
10	35.217861572308465	36.0	36.0	36.0	36.0	36.0
11	35.3249318014466	36.0	36.0	36.0	36.0	36.0
12	35.25390182707636	36.0	36.0	36.0	36.0	36.0
13	35.286570771277276	36.0	36.0	36.0	36.0	36.0
14	35.250783214832566	36.0	36.0	36.0	36.0	36.0
15	35.230590219300154	36.0	36.0	36.0	36.0	36.0
16	35.24478781589165	36.0	36.0	36.0	36.0	36.0
17	35.224498465063895	36.0	36.0	36.0	36.0	36.0
18	35.21702961744323	36.0	36.0	36.0	36.0	36.0
19	35.209438169894256	36.0	36.0	36.0	36.0	36.0
20	35.21242818343649	36.0	36.0	36.0	36.0	36.0
21	35.20285714071473	36.0	36.0	36.0	36.0	36.0
22	35.19284368846571	36.0	36.0	36.0	36.0	36.0
23	35.16215771374754	36.0	36.0	36.0	36.0	36.0
24	35.147714167452754	36.0	36.0	36.0	36.0	36.0
25	35.13497614737786	36.0	36.0	36.0	36.0	36.0
26	35.09536174852243	36.0	36.0	36.0	36.0	36.0
27	35.08248838112606	36.0	36.0	36.0	36.0	36.0
28	35.06381532476608	36.0	36.0	36.0	36.0	36.0
29	35.04935003291827	36.0	36.0	36.0	36.0	36.0
30	35.028538788817684	36.0	36.0	36.0	36.0	36.0
31	35.02951471424094	36.0	36.0	36.0	36.0	36.0
32	35.00552562001071	36.0	36.0	36.0	36.0	36.0
33	34.981781725637106	36.0	36.0	36.0	36.0	36.0
34	34.977626825314225	36.0	36.0	36.0	36.0	36.0
35	34.9661470479287	36.0	36.0	36.0	36.0	36.0
36	34.94680137911797	36.0	36.0	36.0	36.0	36.0
37	34.93755614476839	36.0	36.0	36.0	36.0	36.0
38	34.923229574552	36.0	36.0	36.0	36.0	36.0
39	34.90336876108836	36.0	36.0	36.0	36.0	36.0
40	34.8910888973205	36.0	36.0	36.0	36.0	36.0
41	34.870633830381685	36.0	36.0	36.0	32.0	36.0
42	34.83854714210944	36.0	36.0	36.0	32.0	36.0
43	34.839281241986015	36.0	36.0	36.0	32.0	36.0
44	34.797511408916876	36.0	36.0	36.0	32.0	36.0
45	34.77903481238087	36.0	36.0	36.0	32.0	36.0
46	34.75792063023112	36.0	36.0	36.0	32.0	36.0
47	34.73384305409544	36.0	36.0	36.0	32.0	36.0
48	34.71203226440193	36.0	36.0	36.0	32.0	36.0
49	34.69999647572072	36.0	36.0	36.0	32.0	36.0
50	34.30386373486622	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	9.0
20	22.0
21	69.0
22	187.0
23	549.0
24	1386.0
25	3150.0
26	6448.0
27	12039.0
28	20699.0
29	32239.0
30	47216.0
31	68888.0
32	104049.0
33	174557.0
34	402909.0
35	1792793.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.81318852765812	17.21665120249267	11.670663349307562	26.29949692054165
2	16.533419019762746	19.62457945722846	37.00825131819748	26.833750204811313
3	19.428637201081884	22.94861806492347	27.70247622789725	29.920268506097404
4	13.022211957654658	15.445191458346768	34.82032924266987	36.7122673413287
5	14.957614885966986	36.395221973522894	32.74830253335883	15.898860607151294
6	35.53712346393682	34.29608789415749	16.222356861557106	13.94443178034859
7	31.384606847899605	29.837673195831453	19.492113862715073	19.285606093553866
8	28.406440882839455	33.06718776010305	18.50396593896548	20.022405418092003
9	27.093796818550608	14.721177019299553	18.050983573859146	40.1340425882907
10	16.29069937901379	26.58096909541022	30.801035839336176	26.32729568623981
11	37.545384468876115	21.22343480758185	21.715296721820387	19.51588400172165
12	25.362213427354106	23.15342762405088	27.911429612644213	23.5729293359508
13	29.611219505611103	19.337457989841077	24.84991819172979	26.201404312818028
14	23.863682376953914	19.04329314655153	24.76094888595282	32.332075590541734
15	25.403342516455385	26.622180764033754	21.983404393801468	25.991072325709393
16	26.114436905532735	25.796687836190703	22.999258772967444	25.08961648530912
17	24.48414299275798	25.84650189036342	24.5623519990162	25.107003117862398
18	25.147991888143668	25.150841310779406	25.248846452487566	24.452320348589367
19	25.776428720326695	24.769459645502494	25.091781230738313	24.362330403432498
20	26.07607228554289	24.230728854229156	24.616564187162567	25.076634673065385
21	26.601195031064286	24.10836637971274	24.604877529374317	24.68556105984866
22	26.282200290040684	24.03198545897364	24.69113816224582	24.994676088739855
23	24.776142569897917	24.161530527581057	25.30718417103109	25.755142731489933
24	24.952506142238995	24.5673437970416	25.159578237296447	25.320571823422956
25	25.190330255705785	24.125358146314905	25.197078890794337	25.48723270718497
26	24.962038870696706	24.98517172677585	25.27555093315017	24.777238469377274
27	25.416485140608025	24.60177369393094	24.787061497404398	25.194679668056637
28	25.163347109467587	24.48131411048327	25.259366377809013	25.095972402240125
29	24.983315836832634	24.75978554289142	25.238377324535094	25.018521295740854
30	24.99413237511707	24.818553542358252	25.24780873267551	24.939505349849167
31	25.41929770493229	24.594799486202373	24.72388626566157	25.26201654320377
32	25.348315365018305	24.578330418285876	24.56430819643858	25.509046020257237
33	24.842991188910347	24.464392902388155	25.033527581473802	25.659088327227696
34	25.484798608273124	24.551306806039314	25.080028044496267	24.883866541191292
35	25.649358561702513	24.383316043099732	25.21313477871438	24.754190616483374
36	24.975151909844268	24.775467370923966	24.93443516007569	25.314945559156076
37	25.650970879246543	24.609417472452076	24.818627983968025	24.920983664333356
38	25.053988959257833	24.514961690334328	25.09909223563781	25.33195711477003
39	25.61643342563994	24.526638067365077	24.596299187386915	25.260629319608068
40	25.590083116846113	24.600021970531742	24.901085741687286	24.908809170934862
41	24.863770280737825	25.004517870144788	25.208215699992426	24.923496149124958
42	25.827513623482705	24.78540249545759	24.77752873360664	24.60955514745306
43	25.48240544140965	24.258605685644685	24.973688680189703	25.28530019275596
44	25.04067156162674	24.72343525563562	24.812783337088977	25.423109845648657
45	25.109901584419976	24.91981167976862	24.81239401175558	25.15789272405582
46	25.094435902252325	24.653216369664584	24.840532207938576	25.411815520144515
47	25.21396121534694	24.702036488602793	25.02222377191868	25.061778524131583
48	25.540555994023507	25.168693513403056	24.38679996925517	24.903950523318265
49	25.104739151333543	25.115987315070814	24.450583442253052	25.328690091342587
50	25.17495102496087	25.198459043744787	24.65784959742049	24.968740333873853
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	131.0
1	104.0
2	77.0
3	212.5
4	348.0
5	394.5
6	441.0
7	373.5
8	306.0
9	427.0
10	548.0
11	892.0
12	1236.0
13	2159.0
14	3082.0
15	4064.5
16	5047.0
17	4849.5
18	4652.0
19	4460.5
20	4269.0
21	4783.5
22	5298.0
23	6121.5
24	6945.0
25	8550.0
26	10155.0
27	12953.5
28	15752.0
29	19593.0
30	23434.0
31	28403.0
32	33372.0
33	39567.5
34	45763.0
35	54336.0
36	62909.0
37	74570.0
38	86231.0
39	98008.0
40	109785.0
41	122600.0
42	135415.0
43	144407.0
44	153399.0
45	167748.0
46	182097.0
47	192019.5
48	201942.0
49	211452.0
50	220962.0
51	217837.5
52	214713.0
53	212838.5
54	210964.0
55	208886.0
56	206808.0
57	197392.5
58	187977.0
59	172696.5
60	157416.0
61	138106.0
62	118796.0
63	101820.0
64	84844.0
65	71283.5
66	57723.0
67	49201.0
68	40679.0
69	35311.5
70	29944.0
71	23056.0
72	16168.0
73	13996.5
74	11825.0
75	8804.5
76	5784.0
77	4928.0
78	4072.0
79	3348.5
80	2625.0
81	2023.0
82	1421.0
83	1200.0
84	979.0
85	771.5
86	564.0
87	374.5
88	185.0
89	124.5
90	64.0
91	43.5
92	23.0
93	20.5
94	18.0
95	14.5
96	11.0
97	9.0
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0312686055701609
2	0.0046115569366064636
3	3.749233281793873E-4
4	0.0
5	7.498466563587746E-5
6	1.4996933127175491E-4
7	0.0
8	0.0
9	0.0
10	1.1247699845381619E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.8740032663320353E-4
17	0.0
18	1.8746166408969365E-4
19	0.0
20	4.4990799381526477E-4
21	3.749233281793873E-5
22	0.0
23	3.749233281793873E-5
24	9.373083204484682E-4
25	2.2495399690763239E-4
26	3.749233281793873E-4
27	3.749233281793873E-4
28	0.0014622009798996105
29	4.4990799381526477E-4
30	0.0012747393158099168
31	5.248926594511422E-4
32	6.373696579049584E-4
33	1.8746166408969365E-4
34	8.24831321994652E-4
35	1.1247699845381619E-4
36	1.8746166408969365E-4
37	0.0015746779783534267
38	0.0
39	5.248926594511422E-4
40	1.1247699845381619E-4
41	9.748006532664071E-4
42	0.00472403393506028
43	0.0040866642771553215
44	0.004311618274062954
45	0.0019870936393507526
46	0.002212047636258385
47	8.623236548125909E-4
48	0.0036367562833400572
49	0.004124156609973261
50	0.001387216314263733
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2667212.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.61674988887663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.64616475609658	37.497589166015615
2	15.302020957496318	15.79681177114872
3	5.301698642435207	8.209693584383247
4	2.297590919773397	4.743767034115899
5	1.18732067174904	3.06428170757816
6	0.7284268303222848	2.2559415307855537
7	0.487152883869038	1.760167399501917
8	0.3417998283053466	1.411407699975844
9	0.24705849920141398	1.1477121085080553
>10	1.3201568217608393	12.145703708974903
>50	0.08072668401080779	2.861008520360492
>100	0.05161331511192613	5.251501470908111
>500	0.005927472383251272	2.066870789361539
>1k	0.002341717484722878	1.7875435083820383
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.498466563587746E-5
7	0.0	0.0	0.0	0.0	1.1247699845381619E-4
8	0.0	0.0	0.0	0.0	1.1247699845381619E-4
9	0.0	0.0	0.0	0.0	1.1247699845381619E-4
10	0.0	0.0	0.0	0.0	1.4996933127175491E-4
11	0.0	0.0	0.0	0.0	1.4996933127175491E-4
12	0.0	0.0	0.0	3.749233281793873E-5	0.0010497853189022845
13	0.0	0.0	0.0	3.749233281793873E-5	0.001124769984538162
14	0.0	0.0	0.0	3.749233281793873E-5	0.001124769984538162
15	0.0	0.0	0.0	7.498466563587746E-5	0.001387216314263733
16	0.0	0.0	0.0	1.4996933127175491E-4	0.0014622009798996105
17	0.0	0.0	0.0	1.8746166408969365E-4	0.0015746779783534267
18	0.0	0.0	0.0	3.374309953614486E-4	0.0016496626439893042
19	0.0	0.0	0.0	4.1241566099732606E-4	0.0016496626439893042
20	0.0	0.0	0.0	5.62384992269081E-4	0.001837124308078998
21	0.0	0.0	0.0	6.748619907228972E-4	0.0019121089737148753
22	0.0	0.0	0.0	7.498466563587746E-4	0.0019870936393507526
23	0.0	0.0	0.0	8.623236548125908E-4	0.0020245859721686917
24	0.0	0.0	0.0	0.0011622623173561007	0.002099570637804569
25	0.0	0.0	0.0	0.0017621396424431204	0.002099570637804569
26	0.0	0.0	0.0	0.002511986298801895	0.002249539969076324
27	0.0	0.0	0.0	0.00326183295516067	0.0022870323018942627
28	0.0	0.0	0.0	0.004499079938152648	0.0023245246347122013
29	0.0	0.0	0.0	0.006036265583688136	0.0023245246347122013
30	0.0	0.0	0.0	0.007723420560495378	0.0023245246347122013
31	0.0	0.0	0.0	0.015559318119444573	0.002511986298801895
32	0.0	0.0	0.0	0.02508237065520101	0.0025494786316198337
33	0.0	0.0	0.0	0.034492946192503635	0.0025869709644377723
34	0.0	0.0	0.0	0.04495330704870854	0.00266195563007365
35	0.0	0.0	0.0	0.05612602222845428	0.00266195563007365
36	0.0	0.0	0.0	0.07209775600889617	0.0027369402957095274
37	0.0	0.0	0.0	0.09819241965018154	0.0027369402957095274
38	0.0	0.0	0.0	0.12979845621570388	0.002774432628527466
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3485	0.0	27.023924	1
TAATCGG	75	5.4778586E-5	20.532335	15
GGTATCA	1525	0.0	20.200665	1
TCAACGC	4885	0.0	19.274757	4
CAACGCA	4945	0.0	19.129864	5
AACGCAG	4985	0.0	19.020496	6
ATCAACG	4965	0.0	19.00814	3
TATCAAC	5010	0.0	18.926292	2
CTAGCGG	495	0.0	18.66681	29
ATACGAA	485	0.0	18.599823	40
CGCAATA	480	0.0	18.333818	36
CAATACG	500	0.0	18.041492	38
TACGAAT	505	0.0	17.863197	41
TCTAGCG	520	0.0	17.346287	28
CGAATGC	530	0.0	17.021233	43
TAGCGGC	540	0.0	16.70383	30
GTGTAGG	515	0.0	16.663446	1
AATACGA	530	0.0	16.605457	39
ACGCAGA	5940	0.0	15.888415	7
GTATAGG	420	0.0	15.717353	1
>>END_MODULE