FastQCFastQC Report
Thu 2 Feb 2017
SRR4062298_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062298_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683210
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15770.23082214838775778No Hit
TATCAACGCAGAGTACTTTTTTTTT11240.1645174982801774No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA7560.11065411806033285No Hit
GGTATCAACGCAGAGTACTTTTTTT7130.10436029917594884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG508.705966E-515.19929518
TAGGACG602.563443E-514.2503854
ATCGCCA759.654868E-713.93268816
GTCCTAC1600.013.6526161
GCATCGC707.246248E-613.57179514
CGCAAGA852.695433E-713.410162
CCTATTC1001.0029908E-813.3003583
GAGTAAG500.001501052313.2964611
TTAGAGT801.9941126E-613.0628524
CGGACCA957.353265E-813.0013049
CAAGACG905.3883196E-712.6670084
CCCGAGG604.0909238E-412.6651532
GGTATCA3650.012.2296221
CGCATCG701.088897E-412.21461613
TCGCCAG701.0896424E-412.2137217
TACCCAT550.003064792412.0912365
AAGACCG550.003064792412.0912365
CTTATCC550.003064792412.0912363
GACGGAC951.0365857E-612.0003237
CCTGAAC951.0365857E-612.0003233
Produced by FastQC (version 0.11.5)