##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062298_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	683210
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22868810468231	32.0	32.0	32.0	32.0	32.0
2	30.83187599713119	32.0	32.0	32.0	32.0	32.0
3	30.865050277367136	32.0	32.0	32.0	32.0	32.0
4	30.86366124617614	32.0	32.0	32.0	32.0	32.0
5	30.833663148958593	32.0	32.0	32.0	32.0	32.0
6	34.4486673204432	36.0	36.0	36.0	32.0	36.0
7	34.37606153305719	36.0	36.0	36.0	32.0	36.0
8	34.344441679717804	36.0	36.0	36.0	32.0	36.0
9	34.44569751613706	36.0	36.0	36.0	32.0	36.0
10	34.18569546698672	36.0	36.0	36.0	32.0	36.0
11	34.4308850865766	36.0	36.0	36.0	32.0	36.0
12	34.27640988861404	36.0	36.0	36.0	32.0	36.0
13	34.346225904187584	36.0	36.0	36.0	32.0	36.0
14	34.27643916219025	36.0	36.0	36.0	32.0	36.0
15	34.22921649273283	36.0	36.0	36.0	32.0	36.0
16	34.2414777301269	36.0	36.0	36.0	32.0	36.0
17	34.17284875806853	36.0	36.0	36.0	32.0	36.0
18	34.18571595849007	36.0	36.0	36.0	32.0	36.0
19	34.18747969145651	36.0	36.0	36.0	32.0	36.0
20	34.16639100715739	36.0	36.0	36.0	32.0	36.0
21	34.14896883827813	36.0	36.0	36.0	32.0	36.0
22	34.11314237203788	36.0	36.0	36.0	32.0	36.0
23	34.09852314808038	36.0	36.0	36.0	32.0	36.0
24	34.0815517922747	36.0	36.0	36.0	32.0	36.0
25	33.71519152237233	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	6.0
5	45.0
6	129.0
7	31.0
8	123.0
9	95.0
10	46.0
11	17.0
12	29.0
13	29.0
14	133.0
15	199.0
16	289.0
17	403.0
18	529.0
19	710.0
20	1017.0
21	1378.0
22	2002.0
23	2832.0
24	4149.0
25	5511.0
26	7547.0
27	9796.0
28	12831.0
29	16912.0
30	22460.0
31	29979.0
32	43366.0
33	61325.0
34	137666.0
35	321626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.384314764425795	17.3375995128827	11.32269618254018	25.95538954015132
2	16.5970931338827	19.617979830505995	37.504573996282254	26.28035303932905
3	19.247404527750657	23.046534808528577	28.22855085339918	29.477509810321585
4	12.927798954252959	15.373535456445747	35.28166663739044	36.41699895191086
5	14.779898155495886	36.36534007221062	33.54177586595647	15.312985906337023
6	34.546700781946825	34.90332946192275	16.53512170045241	14.014848055678012
7	30.453702957530826	30.245349782782295	20.232529176037918	19.068418083648965
8	27.82209263139387	33.62745241688523	19.277205526820907	19.273249424899998
9	26.980340207031574	14.68784070526723	18.635479068240702	39.69634001946049
10	16.292516059627644	27.11691760674541	31.386880570305483	25.203685763321467
11	36.801952334887	21.502799519244864	21.957904611145313	19.737343534722836
12	24.03651249400912	23.859167769574974	29.076620249249952	23.027699487165957
13	29.536917482878245	19.78268229376582	24.947861630703528	25.73253859265241
14	23.03925232001477	19.645795692165215	25.40535233598708	31.90959965183293
15	24.91359928241236	27.378547998933	22.32729383279153	25.380558885863113
16	25.24553120026729	26.159074323195554	23.901301000296016	24.69409347624114
17	23.677083516522167	26.28554596134569	25.389679287644572	24.64769123448757
18	24.19733693605511	25.585723140405044	26.14166374824712	24.075276175292736
19	25.11570394440936	25.333706437959393	25.63258082427798	23.91800879335327
20	25.297067488635182	24.81551856241457	25.327393204402647	24.560020744547597
21	25.83203194815164	24.678511469280963	24.9909904102806	24.498466172286797
22	25.502141307230335	24.728252508691963	25.14142297667345	24.62818320740425
23	24.260751999085965	24.879044130033236	25.518282902706513	25.341920968174286
24	24.413444733052344	25.115919289409263	25.75100316882683	24.719632808711562
25	24.549306049978465	24.97179958219433	25.62839320611649	24.850501161710717
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	879.0
1	879.0
2	594.5
3	310.0
4	310.0
5	310.0
6	664.0
7	1018.0
8	1018.0
9	1018.0
10	1196.0
11	1374.0
12	1374.0
13	1374.0
14	1722.5
15	2071.0
16	2071.0
17	2071.0
18	3426.0
19	4781.0
20	4781.0
21	4781.0
22	7321.0
23	9861.0
24	9861.0
25	9861.0
26	14305.0
27	18749.0
28	18749.0
29	18749.0
30	23935.0
31	29121.0
32	29121.0
33	29121.0
34	35436.0
35	41751.0
36	41751.0
37	41751.0
38	49139.0
39	56527.0
40	56527.0
41	56527.0
42	64594.0
43	72661.0
44	72661.0
45	72661.0
46	80804.0
47	88947.0
48	88947.0
49	88947.0
50	91267.0
51	93587.0
52	93587.0
53	93587.0
54	87886.5
55	82186.0
56	82186.0
57	82186.0
58	74881.0
59	67576.0
60	67576.0
61	67576.0
62	58763.5
63	49951.0
64	49951.0
65	49951.0
66	40603.0
67	31255.0
68	31255.0
69	31255.0
70	23578.5
71	15902.0
72	15902.0
73	15902.0
74	11750.5
75	7599.0
76	7599.0
77	7599.0
78	5950.5
79	4302.0
80	4302.0
81	4302.0
82	2931.0
83	1560.0
84	1560.0
85	1560.0
86	1124.5
87	689.0
88	689.0
89	689.0
90	461.5
91	234.0
92	234.0
93	234.0
94	150.5
95	67.0
96	67.0
97	67.0
98	159.5
99	252.0
100	252.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010245751672253041
2	0.0
3	1.463678810321863E-4
4	0.00907480862399555
5	0.030444519254694748
6	0.06249908520074355
7	0.1046530349380132
8	0.10538487434317413
9	0.11738704058781341
10	0.13304840385825734
11	0.13904948698057698
12	0.13509755419270794
13	0.13041378199967799
14	0.11402057932407311
15	0.13524392207374014
16	0.11885071939813528
17	0.1249981704014871
18	0.11167869322755815
19	0.0949927547898889
20	0.09089445412098769
21	0.08840620014344053
22	0.09996926274498324
23	0.07655040177983342
24	0.09118718988305206
25	0.08723525709518304
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	683210.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.65090302525681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.3785910697597	51.96548496557807
2	12.694803851790201	16.4146106549349
3	3.273666496574873	6.34936485621283
4	1.2795716874117076	3.309018603068741
5	0.6622543389784116	2.140767052367442
6	0.37604443186420894	1.458696725858446
7	0.24749418382702348	1.1200505734541186
8	0.17597891193503035	0.9101756456001484
9	0.13469888544161893	0.7837564122266654
>10	0.6818674937698566	7.964901132405674
>50	0.05660814663843634	2.5296451831444493
>100	0.03705646051763382	4.2880692520981905
>500	9.093609941983206E-4	0.36847265173330734
>1k	4.546804970991603E-4	0.3969862913169777
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1577	0.23082214838775778	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1124	0.1645174982801774	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	756	0.11065411806033285	No Hit
GGTATCAACGCAGAGTACTTTTTTT	713	0.10436029917594884	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.463678810321863E-4
2	0.0	0.0	0.0	0.0	1.463678810321863E-4
3	0.0	0.0	0.0	0.0	1.463678810321863E-4
4	0.0	0.0	0.0	1.463678810321863E-4	1.463678810321863E-4
5	0.0	0.0	0.0	1.463678810321863E-4	1.463678810321863E-4
6	0.0	0.0	0.0	1.463678810321863E-4	1.463678810321863E-4
7	0.0	0.0	0.0	1.463678810321863E-4	1.463678810321863E-4
8	0.0	0.0	0.0	1.463678810321863E-4	1.463678810321863E-4
9	0.0	0.0	0.0	1.463678810321863E-4	1.463678810321863E-4
10	0.0	0.0	0.0	2.927357620643726E-4	1.463678810321863E-4
11	0.0	0.0	0.0	2.927357620643726E-4	1.463678810321863E-4
12	0.0	0.0	0.0	2.927357620643726E-4	2.927357620643726E-4
13	0.0	0.0	0.0	2.927357620643726E-4	4.391036430965589E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGCG	50	8.705966E-5	15.199295	18
TAGGACG	60	2.563443E-5	14.250385	4
ATCGCCA	75	9.654868E-7	13.932688	16
GTCCTAC	160	0.0	13.652616	1
GCATCGC	70	7.246248E-6	13.571795	14
CGCAAGA	85	2.695433E-7	13.41016	2
CCTATTC	100	1.0029908E-8	13.300358	3
GAGTAAG	50	0.0015010523	13.296461	1
TTAGAGT	80	1.9941126E-6	13.062852	4
CGGACCA	95	7.353265E-8	13.001304	9
CAAGACG	90	5.3883196E-7	12.667008	4
CCCGAGG	60	4.0909238E-4	12.665153	2
GGTATCA	365	0.0	12.229622	1
CGCATCG	70	1.088897E-4	12.214616	13
TCGCCAG	70	1.0896424E-4	12.21372	17
TACCCAT	55	0.0030647924	12.091236	5
AAGACCG	55	0.0030647924	12.091236	5
CTTATCC	55	0.0030647924	12.091236	3
GACGGAC	95	1.0365857E-6	12.000323	7
CCTGAAC	95	1.0365857E-6	12.000323	3
>>END_MODULE