FastQCFastQC Report
Thu 2 Feb 2017
SRR4062298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683210
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7910.11577699389645937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGC300.00574216729.33450531
CGGATCG454.067907E-529.3323626
TAGGACG953.274181E-1127.788554
ATCGATT508.333953E-526.40105429
TCGTCTA450.001397862924.4436329
GTATCAA9900.022.0040991
GGTATCA6150.021.1096231
AACGCTT1057.730523E-820.95321730
TCGATTT654.927818E-420.30850230
GTATAGG1001.1199681E-619.803691
AACCGCG901.0005697E-519.55633741
TTCGTTA708.116208E-418.85789540
CGATTTT708.116208E-418.85789531
CCCTCGG708.120203E-418.85651422
GGATCGA708.120203E-418.85651427
GTGTAAG1303.572859E-818.6188531
TAAACGC1254.842823E-717.60070428
CCTATTC1751.9099389E-1017.5994153
CGAGCCG2150.017.3947715
CGCGGGA1154.2657703E-617.21807944
Produced by FastQC (version 0.11.5)