##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062298_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	683210
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24183486775662	32.0	32.0	32.0	32.0	32.0
2	31.430381581065852	32.0	32.0	32.0	32.0	32.0
3	31.562683508730846	32.0	32.0	32.0	32.0	32.0
4	31.63973156130619	32.0	32.0	32.0	32.0	32.0
5	31.619655742743813	32.0	32.0	32.0	32.0	32.0
6	35.25974590535853	36.0	36.0	36.0	36.0	36.0
7	35.25645994642935	36.0	36.0	36.0	36.0	36.0
8	35.20854056585823	36.0	36.0	36.0	36.0	36.0
9	35.282415362772795	36.0	36.0	36.0	36.0	36.0
10	35.17665578665417	36.0	36.0	36.0	36.0	36.0
11	35.28814127427877	36.0	36.0	36.0	36.0	36.0
12	35.20912164634593	36.0	36.0	36.0	36.0	36.0
13	35.24539599830213	36.0	36.0	36.0	36.0	36.0
14	35.20904407136898	36.0	36.0	36.0	36.0	36.0
15	35.19650766235857	36.0	36.0	36.0	36.0	36.0
16	35.20017417777843	36.0	36.0	36.0	36.0	36.0
17	35.184318145226214	36.0	36.0	36.0	36.0	36.0
18	35.17498280177398	36.0	36.0	36.0	36.0	36.0
19	35.156773173694766	36.0	36.0	36.0	36.0	36.0
20	35.16116713748335	36.0	36.0	36.0	36.0	36.0
21	35.160413342896035	36.0	36.0	36.0	36.0	36.0
22	35.14302044759298	36.0	36.0	36.0	36.0	36.0
23	35.106036211413766	36.0	36.0	36.0	36.0	36.0
24	35.09299922425023	36.0	36.0	36.0	36.0	36.0
25	35.08294375082332	36.0	36.0	36.0	36.0	36.0
26	35.03255660777799	36.0	36.0	36.0	36.0	36.0
27	35.02148241389909	36.0	36.0	36.0	36.0	36.0
28	35.01056776101052	36.0	36.0	36.0	36.0	36.0
29	34.98721183823422	36.0	36.0	36.0	36.0	36.0
30	34.95747134848729	36.0	36.0	36.0	36.0	36.0
31	34.9677536921298	36.0	36.0	36.0	36.0	36.0
32	34.93492044905666	36.0	36.0	36.0	36.0	36.0
33	34.91651761537448	36.0	36.0	36.0	32.0	36.0
34	34.8997306830989	36.0	36.0	36.0	32.0	36.0
35	34.88775486307285	36.0	36.0	36.0	32.0	36.0
36	34.86771710015954	36.0	36.0	36.0	32.0	36.0
37	34.845719471319214	36.0	36.0	36.0	32.0	36.0
38	34.83284787986124	36.0	36.0	36.0	32.0	36.0
39	34.808080970711785	36.0	36.0	36.0	32.0	36.0
40	34.79823333967594	36.0	36.0	36.0	32.0	36.0
41	34.765670877182714	36.0	36.0	36.0	32.0	36.0
42	34.745516019964576	36.0	36.0	36.0	32.0	36.0
43	34.73046793811566	36.0	36.0	36.0	32.0	36.0
44	34.692336177749155	36.0	36.0	36.0	32.0	36.0
45	34.66477510575079	36.0	36.0	36.0	32.0	36.0
46	34.646138083458965	36.0	36.0	36.0	32.0	36.0
47	34.617619765518654	36.0	36.0	36.0	32.0	36.0
48	34.5885847689583	36.0	36.0	36.0	32.0	36.0
49	34.56978381463971	36.0	36.0	36.0	32.0	36.0
50	34.17282533920756	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	10.0
21	12.0
22	52.0
23	169.0
24	341.0
25	823.0
26	1764.0
27	3076.0
28	5634.0
29	8893.0
30	13278.0
31	19475.0
32	28765.0
33	49219.0
34	110635.0
35	441059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87834881889072	17.827766243729886	11.842717883695801	26.451167053683587
2	16.109375777606683	19.8881417359255	37.303311250523286	26.69917123594453
3	18.54296202620578	23.33066943010035	28.667550731256075	29.458817812437793
4	12.652917843708378	15.876231319799183	35.252265042958975	36.218585793533464
5	14.354005357064445	36.83274542234452	33.31552524114109	15.497723979449948
6	34.800478330935334	34.91815827953086	16.43420973669843	13.84715365283537
7	30.520045081307355	30.22043002883447	20.267414118645803	18.992110771212364
8	27.942653064211587	33.58762313197992	19.20536877387626	19.264355029932233
9	27.16207315466694	14.290920800339574	18.666588603796782	39.880417441196705
10	16.26105627999631	27.04399385839472	31.573208198369752	25.121741663239217
11	37.175978103365	21.204607660894894	21.95942682337788	19.65998741236223
12	24.233398223093925	23.740138464015455	28.965471816864508	23.060991496026112
13	29.75380922410386	19.601293892068323	25.061694061855068	25.583202821972744
14	23.257856296014403	19.44921766367588	25.524802037440903	31.76812400286881
15	24.868195723130516	27.261456945887797	22.43819616223416	25.43215116874753
16	25.41473581906482	25.913267740622885	23.93055681595302	24.74143962435927
17	23.776437698511437	26.17994467294097	25.508701570527364	24.53491605802023
18	24.562433219654277	25.339939403697255	26.233515317398748	23.864112059249717
19	25.220210477012923	25.074867171147964	25.865253728721772	23.839668623117344
20	25.483087191346733	24.656694135039007	25.455570029712682	24.404648643901584
21	26.01528080677976	24.461000278098975	25.107799944380204	24.41591897074106
22	25.47620790093822	24.50813073579134	25.44781253201797	24.56784883125247
23	24.424847411484023	24.580875572664336	25.690051375126245	25.3042256407254
24	24.586322326811583	24.773382942940657	25.892233764294843	24.74806096595292
25	24.800207257526257	24.498103066708822	25.837958571913678	24.863731103851244
26	24.43347979965047	25.14556370992058	26.020848763037797	24.400107727391152
27	24.75238104995997	25.01481981302885	25.453779015730227	24.779020121280958
28	24.584124344808306	24.977715066079035	25.76416465528976	24.673995933822894
29	24.502640484303463	25.135390686291732	25.666414913174318	24.69555391623049
30	24.396666295864776	25.09440811236468	25.862553497930342	24.6463720938402
31	24.9134963290828	24.79801173288369	25.467793117176612	24.820698820856897
32	24.693833640463286	25.082734808045	25.203049734560683	25.02038181693103
33	24.40508774754468	24.875514117182128	25.592570366358807	25.12682776891439
34	24.7948630126961	24.951478479278457	25.93581400522832	24.317844502797122
35	25.145746030863176	24.86632933699644	25.75434457098779	24.23358006115259
36	24.43849539452788	25.219954655164084	25.59641339619355	24.74513655411448
37	24.99436470460806	25.12609888260143	25.39717854665	24.48235786614051
38	24.62932334128599	24.99216931836478	25.578958153422814	24.79954918692642
39	24.990083474457812	24.820002254088465	25.351323106016803	24.83859116543692
40	25.122912608001357	24.979471874638655	25.474927877121058	24.422687640238934
41	24.28560764516549	25.22734824056686	25.858785748891616	24.62825836537603
42	25.137079941098804	25.227685903234587	25.677934593912568	23.957299561754038
43	24.91663958734034	24.65784905251633	25.639145288636346	24.786366071506986
44	24.301248713741412	25.359530316181754	25.466968586747623	24.87225238332921
45	24.626495372450748	25.395094490413367	25.275947721793756	24.702462415342122
46	24.52798704900972	25.170559916626534	25.49492016141899	24.806532872944757
47	24.68106158629048	25.1310004039789	25.71530611647473	24.472631893255894
48	25.049840597911526	25.49042855595467	25.005342677896536	24.45438816823727
49	24.681896224909945	25.52690397532747	25.090860654957854	24.70033914480473
50	24.77550333360168	25.661487569434787	24.951002276070522	24.612006820893008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	553.0
1	306.5
2	60.0
3	154.5
4	249.0
5	266.5
6	284.0
7	201.0
8	118.0
9	187.0
10	256.0
11	431.0
12	606.0
13	1018.5
14	1431.0
15	1702.5
16	1974.0
17	1907.5
18	1841.0
19	1783.5
20	1726.0
21	1927.0
22	2128.0
23	2569.0
24	3010.0
25	3714.5
26	4419.0
27	5459.0
28	6499.0
29	7616.5
30	8734.0
31	10259.0
32	11784.0
33	13413.5
34	15043.0
35	17031.0
36	19019.0
37	21831.5
38	24644.0
39	26992.0
40	29340.0
41	32223.0
42	35106.0
43	36975.5
44	38845.0
45	42009.5
46	45174.0
47	47417.0
48	49660.0
49	51325.5
50	52991.0
51	52545.5
52	52100.0
53	51341.0
54	50582.0
55	50102.0
56	49622.0
57	47775.0
58	45928.0
59	41770.0
60	37612.0
61	33136.0
62	28660.0
63	24670.0
64	20680.0
65	17567.5
66	14455.0
67	12110.0
68	9765.0
69	8463.5
70	7162.0
71	5625.0
72	4088.0
73	3524.5
74	2961.0
75	2219.0
76	1477.0
77	1288.0
78	1099.0
79	878.0
80	657.0
81	521.5
82	386.0
83	310.5
84	235.0
85	190.5
86	146.0
87	94.5
88	43.0
89	32.5
90	22.0
91	16.5
92	11.0
93	10.5
94	10.0
95	6.5
96	3.0
97	5.5
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030444519254694748
2	0.0035128291447724712
3	2.927357620643726E-4
4	0.0
5	0.0
6	1.463678810321863E-4
7	0.0
8	0.0
9	0.0
10	1.463678810321863E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.854715241287452E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0013173109292896766
25	2.927357620643726E-4
26	5.854715241287452E-4
27	4.391036430965589E-4
28	0.0019027824534184217
29	2.927357620643726E-4
30	8.782072861931178E-4
31	2.927357620643726E-4
32	4.391036430965589E-4
33	0.0
34	0.0011709430482574905
35	1.463678810321863E-4
36	1.463678810321863E-4
37	0.002341886096514981
38	0.0
39	0.0010245751672253041
40	1.463678810321863E-4
41	0.0010245751672253041
42	0.004683772193029962
43	0.004683772193029962
44	0.0039519327878690304
45	0.0027809897396115398
46	0.0027809897396115398
47	8.782072861931178E-4
48	0.004683772193029962
49	0.003073725501675912
50	0.0021955182154827944
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	683210.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.27489843848662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.37482978044261	53.26833680709484
2	12.828948812313982	17.00474559217305
3	3.3236132611649207	6.6081639399753715
4	1.2810312205924972	3.3960085616519318
5	0.6166316717984865	2.0433600711199453
6	0.35184017457532357	1.3990903101935384
7	0.23509633373980923	1.0906689949306487
8	0.16506716750459535	0.8751847809516572
9	0.12190033163419878	0.7271038888806927
>10	0.6225244068366181	7.480949918864333
>50	0.047021552617902605	2.1470427944487036
>100	0.031051691190665268	3.763839310153416
>500	4.4359558835067577E-4	0.19550502956191665
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	791	0.11577699389645937	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.463678810321863E-4	0.0
11	0.0	0.0	0.0	1.463678810321863E-4	0.0
12	0.0	0.0	0.0	1.463678810321863E-4	2.927357620643726E-4
13	0.0	0.0	0.0	1.463678810321863E-4	2.927357620643726E-4
14	0.0	0.0	0.0	1.463678810321863E-4	2.927357620643726E-4
15	0.0	0.0	0.0	2.927357620643726E-4	4.391036430965589E-4
16	0.0	0.0	0.0	2.927357620643726E-4	4.391036430965589E-4
17	0.0	0.0	0.0	2.927357620643726E-4	4.391036430965589E-4
18	0.0	0.0	0.0	2.927357620643726E-4	5.854715241287452E-4
19	0.0	0.0	0.0	2.927357620643726E-4	5.854715241287452E-4
20	0.0	0.0	0.0	2.927357620643726E-4	5.854715241287452E-4
21	0.0	0.0	0.0	2.927357620643726E-4	7.318394051609315E-4
22	0.0	0.0	0.0	5.854715241287452E-4	7.318394051609315E-4
23	0.0	0.0	0.0	0.0010245751672253041	7.318394051609315E-4
24	0.0	0.0	0.0	0.0011709430482574905	7.318394051609315E-4
25	0.0	0.0	0.0	0.0016100466913540493	7.318394051609315E-4
26	0.0	0.0	0.0	0.0020491503344506083	0.0011709430482574905
27	0.0	0.0	0.0	0.002341886096514981	0.0011709430482574905
28	0.0	0.0	0.0	0.004098300668901217	0.0011709430482574905
29	0.0	0.0	0.0	0.007903865575738061	0.0011709430482574905
30	0.0	0.0	0.0	0.01507589174631519	0.0011709430482574905
31	0.0	0.0	0.0	0.025028907656503858	0.0011709430482574905
32	0.0	0.0	0.0	0.03776291330630407	0.0011709430482574905
33	0.0	0.0	0.0	0.05210696564745832	0.0011709430482574905
34	0.0	0.0	0.0	0.06249908520074355	0.0013173109292896766
35	0.0	0.0	0.0	0.07874591999531623	0.0013173109292896766
36	0.0	0.0	0.0	0.0971882730053717	0.0013173109292896766
37	0.0	0.0	0.0	0.12982831047554924	0.001463678810321863
38	0.0	0.0	0.0	0.17637329664378448	0.001463678810321863
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTAGC	30	0.005742167	29.334505	31
CGGATCG	45	4.067907E-5	29.33236	26
TAGGACG	95	3.274181E-11	27.78855	4
ATCGATT	50	8.333953E-5	26.401054	29
TCGTCTA	45	0.0013978629	24.443632	9
GTATCAA	990	0.0	22.004099	1
GGTATCA	615	0.0	21.109623	1
AACGCTT	105	7.730523E-8	20.953217	30
TCGATTT	65	4.927818E-4	20.308502	30
GTATAGG	100	1.1199681E-6	19.80369	1
AACCGCG	90	1.0005697E-5	19.556337	41
TTCGTTA	70	8.116208E-4	18.857895	40
CGATTTT	70	8.116208E-4	18.857895	31
CCCTCGG	70	8.120203E-4	18.856514	22
GGATCGA	70	8.120203E-4	18.856514	27
GTGTAAG	130	3.572859E-8	18.618853	1
TAAACGC	125	4.842823E-7	17.600704	28
CCTATTC	175	1.9099389E-10	17.599415	3
CGAGCCG	215	0.0	17.39477	15
CGCGGGA	115	4.2657703E-6	17.218079	44
>>END_MODULE