FastQCFastQC Report
Thu 2 Feb 2017
SRR4062297_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062297_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52271
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTATAGAGATCACATATCGAGC1440.2754873639302864No Hit
GTATCAACGCAGAGTACTTTTTTTT980.18748445600811156No Hit
CTATAGGCCCGAGCCCCTTGGAAAA950.1817451359262306No Hit
GTCCTGGGAAGGCCACCTTCTGAGG910.17409270915038932No Hit
ATTATAGCCAGTGTATTTATCATGA740.14156989535306383No Hit
GTATATGACTGAAGAACAAATAGCT690.13200436188326223No Hit
GTCCCTGACTGTGCAAGCAGCTTCA670.1281781484953416No Hit
GGTATCAACGCAGAGTACATGGGGG670.1281781484953416No Hit
TATCAACGCAGAGTACTTTTTTTTT670.1281781484953416No Hit
GTTCAGCTCTTTAATCAGCCAGCCA660.12626504180138126No Hit
GTGCAGGCTTGGTTGTTCCAGTAGA660.12626504180138126No Hit
GTATCAACGCAGAGTACATGGGGGG650.12435193510742094No Hit
TCCCAGGACAGCGCCCATCGCTTGT630.12052572171950031No Hit
GTCATATACAACTCACGACATGCCC570.10904708155573836No Hit
TCTTTATTGAGTGTGTGAAGAAACC540.1033077614738574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGAC250.00597677718.9884833
GTACATG1605.4601893E-57.71407131
TACATGG1408.876694E-47.45976162
ACATGGG1450.00128949617.20252853
Produced by FastQC (version 0.11.5)