##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062297_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	52271
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.392569493600657	32.0	32.0	32.0	32.0	32.0
2	31.171911767519276	32.0	32.0	32.0	32.0	32.0
3	31.190162805379657	32.0	32.0	32.0	32.0	32.0
4	31.167932505595836	32.0	32.0	32.0	32.0	32.0
5	31.193089858621416	32.0	32.0	32.0	32.0	32.0
6	34.80893803447418	36.0	36.0	36.0	36.0	36.0
7	34.77488473532169	36.0	36.0	36.0	36.0	36.0
8	34.796273268160164	36.0	36.0	36.0	36.0	36.0
9	34.81389298081154	36.0	36.0	36.0	32.0	36.0
10	34.65336419812133	36.0	36.0	36.0	32.0	36.0
11	34.73983662068834	36.0	36.0	36.0	32.0	36.0
12	34.722178645903085	36.0	36.0	36.0	32.0	36.0
13	34.721700369229595	36.0	36.0	36.0	32.0	36.0
14	34.72942932027319	36.0	36.0	36.0	32.0	36.0
15	34.673528342675674	36.0	36.0	36.0	32.0	36.0
16	34.69103326892541	36.0	36.0	36.0	32.0	36.0
17	34.6614183773029	36.0	36.0	36.0	32.0	36.0
18	34.6595817948767	36.0	36.0	36.0	32.0	36.0
19	34.641349888083255	36.0	36.0	36.0	32.0	36.0
20	34.62658070440588	36.0	36.0	36.0	32.0	36.0
21	34.6171682194716	36.0	36.0	36.0	32.0	36.0
22	34.616422107860956	36.0	36.0	36.0	32.0	36.0
23	34.56981882879608	36.0	36.0	36.0	32.0	36.0
24	34.55944979051482	36.0	36.0	36.0	32.0	36.0
25	34.19949876604618	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	6.0
6	12.0
7	3.0
8	6.0
9	8.0
10	1.0
11	1.0
12	0.0
13	2.0
14	5.0
15	11.0
16	22.0
17	19.0
18	33.0
19	39.0
20	66.0
21	79.0
22	114.0
23	135.0
24	178.0
25	195.0
26	332.0
27	468.0
28	665.0
29	882.0
30	1242.0
31	1809.0
32	2675.0
33	4313.0
34	11075.0
35	27875.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.08618545656291	18.04633544412772	11.706299860343211	25.16117923896616
2	16.425934074343328	20.202406688221004	39.36408333492759	24.007575902508083
3	19.35335756648173	24.2816146929405	29.056820355844653	27.30820738473312
4	14.1900424782825	16.302475986376336	34.671463013279244	34.83601852206192
5	14.682972574687566	37.241392509234274	33.66059979713307	14.415035118945092
6	33.709865788516396	34.52164423426701	17.20242767705003	14.566062300166568
7	29.468543521976443	30.791918031217087	21.68725462031983	18.052283826486644
8	26.452675527636266	34.22836787068602	19.797372352242693	19.521584249435016
9	26.888229096829807	15.96015707307729	18.86217795230342	38.28943587778949
10	16.960095020977413	28.06375601065155	31.305197417575048	23.670951550795994
11	36.40822269479089	22.372933310343505	20.62570645822557	20.593137536640036
12	23.0	25.421455938697317	29.85823754789272	21.720306513409962
13	31.180797670587335	20.75016282901038	23.93011762001456	24.138921880387727
14	22.99986592349978	19.59815357505411	26.217701928786223	31.184278572659885
15	24.03264117692131	28.223890889587004	22.52509386253927	25.218374070952414
16	23.368191220409102	26.896115835440128	24.115145943461272	25.6205470006895
17	23.97870262195239	27.205868270354127	24.390477467297416	24.42495164039607
18	23.678112492100272	27.289963038857078	25.277208571921022	23.754715897121628
19	24.345594852842616	26.677900541906823	25.781743676158015	23.194760929092546
20	25.381536870727455	25.632384198533213	26.534285659575286	22.451793271164046
21	24.976063727930757	25.887556968327523	25.40117191988051	23.735207383861205
22	25.64814460230537	26.09811205146862	24.979895071420366	23.27384827480565
23	24.482567156177602	26.309138601160274	25.367133201861037	23.841161040801087
24	25.118710270353066	26.00903729800107	24.362410967297237	24.509841464348625
25	24.660629152386605	25.784525838135902	25.275230236075743	24.27961477340175
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	9.0
3	17.0
4	17.0
5	17.0
6	33.5
7	50.0
8	50.0
9	50.0
10	61.0
11	72.0
12	72.0
13	72.0
14	110.0
15	148.0
16	148.0
17	148.0
18	261.0
19	374.0
20	374.0
21	374.0
22	524.0
23	674.0
24	674.0
25	674.0
26	1107.0
27	1540.0
28	1540.0
29	1540.0
30	2142.0
31	2744.0
32	2744.0
33	2744.0
34	3332.0
35	3920.0
36	3920.0
37	3920.0
38	4471.0
39	5022.0
40	5022.0
41	5022.0
42	5385.0
43	5748.0
44	5748.0
45	5748.0
46	6400.5
47	7053.0
48	7053.0
49	7053.0
50	6972.5
51	6892.0
52	6892.0
53	6892.0
54	6625.0
55	6358.0
56	6358.0
57	6358.0
58	5415.0
59	4472.0
60	4472.0
61	4472.0
62	3981.0
63	3490.0
64	3490.0
65	3490.0
66	2534.0
67	1578.0
68	1578.0
69	1578.0
70	1256.5
71	935.0
72	935.0
73	935.0
74	821.0
75	707.0
76	707.0
77	707.0
78	500.0
79	293.0
80	293.0
81	293.0
82	186.0
83	79.0
84	79.0
85	79.0
86	69.5
87	60.0
88	60.0
89	60.0
90	40.5
91	21.0
92	21.0
93	21.0
94	11.5
95	2.0
96	2.0
97	2.0
98	11.5
99	21.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001913106693960322
4	0.0172179602456429
5	0.03826213387920644
6	0.07652426775841288
7	0.10713397486177803
8	0.10904708155573836
9	0.12626504180138126
10	0.1377436819651432
11	0.14156989535306383
12	0.1358305752711829
13	0.13200436188326223
14	0.11861261502553998
15	0.1281781484953416
16	0.112873294943659
17	0.1109601882496987
18	0.1033077614738574
19	0.09182912131009546
20	0.09182912131009546
21	0.09374222800405578
22	0.0860898012282145
23	0.08035048114633353
24	0.08226358784029386
25	0.08035048114633353
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	52271.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.728625815461726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.846857268771004	20.78399112318494
2	13.540461631686224	9.404832507508944
3	6.8363355919131825	7.12249622161428
4	4.594281936869939	6.382123931051635
5	2.95818872913568	5.136691473283465
6	2.1263702969206193	4.430755103212106
7	1.8729686553186802	4.553193931625567
8	1.366165372114802	3.7956036808172793
9	1.1788685065829339	3.6846434925675804
>10	5.59687104059935	32.51898758393756
>50	0.07712223874841624	1.911193587266362
>100	0.005508731339172589	0.2754873639302864
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCCTATAGAGATCACATATCGAGC	144	0.2754873639302864	No Hit
GTATCAACGCAGAGTACTTTTTTTT	98	0.18748445600811156	No Hit
CTATAGGCCCGAGCCCCTTGGAAAA	95	0.1817451359262306	No Hit
GTCCTGGGAAGGCCACCTTCTGAGG	91	0.17409270915038932	No Hit
ATTATAGCCAGTGTATTTATCATGA	74	0.14156989535306383	No Hit
GTATATGACTGAAGAACAAATAGCT	69	0.13200436188326223	No Hit
GTCCCTGACTGTGCAAGCAGCTTCA	67	0.1281781484953416	No Hit
GGTATCAACGCAGAGTACATGGGGG	67	0.1281781484953416	No Hit
TATCAACGCAGAGTACTTTTTTTTT	67	0.1281781484953416	No Hit
GTTCAGCTCTTTAATCAGCCAGCCA	66	0.12626504180138126	No Hit
GTGCAGGCTTGGTTGTTCCAGTAGA	66	0.12626504180138126	No Hit
GTATCAACGCAGAGTACATGGGGGG	65	0.12435193510742094	No Hit
TCCCAGGACAGCGCCCATCGCTTGT	63	0.12052572171950031	No Hit
GTCATATACAACTCACGACATGCCC	57	0.10904708155573836	No Hit
TCTTTATTGAGTGTGTGAAGAAACC	54	0.1033077614738574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGGAC	25	0.005976777	18.988483	3
GTACATG	160	5.4601893E-5	7.7140713	1
TACATGG	140	8.876694E-4	7.4597616	2
ACATGGG	145	0.0012894961	7.2025285	3
>>END_MODULE