FastQCFastQC Report
Thu 2 Feb 2017
SRR4062297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52271
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTATAGAGATCACATATCGAGCGAAGATTGTTCCGTGAACCACCTGC1170.2238334831933577No Hit
CTATAGGCCCGAGCCCCTTGGAAAAGGAGCTGCTTGGTTCTTTCGAAGAC1070.2047024162537545No Hit
ATTATAGCCAGTGTATTTATCATGAGTAAATACAACCTAAAACCATAGGT1030.1970499894779132No Hit
TCTTTATTGAGTGTGTGAAGAAACCAATGAAGGATAAGGCAAGGTGGGAG920.17600581584434966No Hit
GTCCTGGGAAGGCCACCTTCTGAGGCCTGAGCTTGGCACCGTGTCAGTCG870.16644028237454803No Hit
GTCCCTGACTGTGCAAGCAGCTTCATGTTCTGCTTAGCTTACTTAAGATT670.1281781484953416No Hit
TCCCAGGACAGCGCCCATCGCTTGTTCATCGTGCATGAGATCTGTGGATG580.1109601882496987No Hit
GCTGAAGGCTGTGTCTGGTACCCGCTACCATGCCCAAGCGGAGCTGCCCG550.10522086816781773No Hit
GTCATGGAGCCTGGATATTTCAGGTTCATATGCATCCATGACCAATGTCC540.1033077614738574No Hit
GTGCAGGCTTGGTTGTTCCAGTAGATAAAAATGATGTTGGTTACCGAGAG530.10139465477989708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGA301.2853206E-436.6610834
CCTATAG301.2853206E-436.6610833
TATCGAG301.2853206E-436.66108318
TGTTCCG301.2853206E-436.66108332
ACATATC301.2853206E-436.66108315
TAGAGAT301.2853206E-436.6610837
ATCGAGC301.2853206E-436.66108319
CCGTGTC250.002323100635.2283539
CACATCC250.002334058535.19464519
GAAGTCC250.002334058535.19464527
CCACATC250.002334058535.19464518
CACATAT401.7889963E-532.99497614
AAATTTG353.1612313E-431.45388843
GAGCGAA353.179073E-431.42378622
TTCCGTG353.179073E-431.42378634
GCCTATA353.179073E-431.4237862
GTTCCGT353.179073E-431.42378633
GCGAAGA353.179073E-431.42378624
CGAAGAT353.179073E-431.42378625
GATTGTT353.179073E-431.42378629
Produced by FastQC (version 0.11.5)